Qiagen QIAgility, Руководство пользователя

Страница: 147 / 334

QIAgility Software
QIAgility User Manual 11/2011 5-85
ID
The “ID” column shows a combination of identifiers
associated with each reaction well. This includes a “Reaction
ID” which can be assigned as a fixed attribute of the well. It
also includes a list of the IDs of samples present in the well
on completion of the run, as determined by simulation of the
pipetting operations. The “label” ID and “contents” IDs are
combined together as a ‘Full ID’ using a ‘|’ character as
separator, with the user-assigned “label” ID listed first.
Concentration
The “Concentration” column displays a concentration value
associated with a reaction well. When a reaction contains just
one sample (or standard) and the starting concentration of
that sample has been defined, the final concentration of that
sample in the reaction well is displayed. When a reaction
contains more than one sample, no final concentration value
can be calculated, so no value is displayed. If desired, the
user can assign a concentration value to the reaction well.
User-assigned concentrations are shown in blue. This is
useful for dilution of samples, or stock wells, which are
supplied initially in reaction plates. If a user-assigned
concentration is entered for a well which also has a valid
calculated concentration, the user-assigned value is shown in
red, to indicate that it is overriding the software-calculated
value.
Note : Concentration values are assumed to be entered in a
single unit common to all values in the run. The unit itself can
be defined as needed for the application (see Section 5.6.5).
Creating sample banks from target wells
Selecting a liquid handling operation in the “Reaction List”
region of the right hand pane highlights the destination wells
associated with the operation when the blue button below the
list is activated. Where the selected liquid handling operation
supports the action, the highlighted wells can be directly
entered into a sample bank as follows. Right-click the liquid
handling operation to show the “Create Sample Bank from