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QIAgility Software 

5-136

 

QIAgility User Manual   11/2011

 

different concentrations of the probe and with 3 different 

concentrations of primer (i.e., a total of 9 reactions). Several 

optimizations are possible using the “Optimisation Wizard”. 

Note

: The user must provide all required concentrations of 

all the variables that are to be optimized. 
This section outlines the “Optimisation Wizard” screens that 

will guide the user through the setup. Instructions are 

displayed on each screen. 

Note

: The “Optimisation Wizard” only provides steps to set 

up an optimized matrix, it does not provide steps for 

preparation of a master mix. 
To set up a PCR reaction using the “Optimisation Wizard”: 

1.

 

Select “Wizard/Optimisation Wizard”, or select 

“Optimisation Wizard” from the splash screen that 

appears when the software is started. 

2.

 

The “Optimisation Wizard” will appear. 

 

 

 

3.

 

Select option “1”, “2”, or “3”. These options define the 

number of variables to be optimized. 

4.

 

Click on the “Next” button to continue.  

 

Содержание QIAgility

Страница 1: ...Technical 0800 914413 France n Orders 01 60 920 926 n Fax 01 60 920 925 n Technical 01 60 920 930 n Offers 01 60 920 928 Germany n Orders 02103 29 12000 n Fax 02103 29 22000 n Technical 02103 29 12400...

Страница 2: ...echnical 0800 914413 France n Orders 01 60 920 926 n Fax 01 60 920 925 n Technical 01 60 920 930 n Offers 01 60 920 928 Germany n Orders 02103 29 12000 n Fax 02103 29 22000 n Technical 02103 29 12400...

Страница 3: ...e safety 1 7 1 8 Symbols on the QIAgility 1 8 2 Introduction 2 1 2 1 About this user manual 2 1 2 2 General information 2 2 2 2 1 Technical assistance 2 2 2 2 2 Policy statement 2 3 2 2 3 Version mana...

Страница 4: ...2 4 4 Unpacking the QIAgility 4 3 4 5 Installing the QIAgility 4 4 4 5 1 UV lamp 4 6 4 5 2 HEPA filter system 4 6 4 5 3 Instrument calibration 4 6 4 5 4 Computer setup and installation of software 4...

Страница 5: ...om setup disk 5 27 5 5 3 Delete multiple calibrations 5 28 5 6 Configure run settings 5 29 5 6 1 Configure tip air intake 5 30 5 6 2 Configure pipet speed 5 30 5 6 3 Set master mix extra volume 5 32 5...

Страница 6: ...126 5 10 2 Optimisation Wizard 5 135 5 10 3 Using Empty Project 5 141 5 10 4 Example reactions 5 142 5 10 5 Checking the reaction setup 5 145 5 10 6 Required volumes for reaction setup 5 145 5 11 Sel...

Страница 7: ...eights 7 7 7 1 4 Optimal height calibration 7 11 7 1 5 Calibrate tip offsets 7 11 7 1 6 Calibrate volumes 7 14 8 Maintenance Procedures 8 1 8 1 Regular maintenance 8 2 8 1 1 Cleaning the QIAgility 8 2...

Страница 8: ...onformity A 5 Appendix B B 1 Liability clause B 1 Appendix C C 1 Safety Information French FR C 1 1 Consignes de s curit C 1 1 1 Utilisation appropri e C 2 1 2 S curit lectrique C 4 1 3 S curit biolog...

Страница 9: ...1 Proben D 5 1 4 Chemikalien D 7 1 4 1 Giftige D mpfe D 7 1 5 Abfallentsorgung D 7 1 6 Gefahren durch mechanische Teile D 8 1 7 Sicherheitshinweise Wartungsarbeiten D 9 1 8 Symbole auf dem QIAgility D...

Страница 10: ...Contents Contents 8 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 11: ...situations that could result in personal injury to you or other persons Details about these circumstances are given in a box like this one CAUTION The term CAUTION is used to inform you about situatio...

Страница 12: ...al injury and material damage W4 Do not attempt to move the QIAgility during operation WARNING CAUTION Explosive atmosphere W5 The QIAgility is not designed for use in an explosive atmosphere WARNING...

Страница 13: ...al is likely to make the instrument dangerous Intentional interruption is prohibited Lethal voltages inside the instrument When the instrument is connected to line power terminals may be live and open...

Страница 14: ...les may contain infectious agents You should be aware of the health hazard presented by such agents and should use store and dispose of such samples according to the required safety regulations WARNIN...

Страница 15: ...all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration United States of America ACGIH American Conference of Government Industrial Hygi...

Страница 16: ...on of the QIAgility Only use recommended tips and tip racks Keep the instrument lid closed whenever possible Do not disable the lid interlock When calibrating plates with the lid open always stand cle...

Страница 17: ...on the QIAgility Risk of personal injury and material damage Only perform maintenance that is specifically described in this user manual CAUTION Damage to the instrument C4 Do not use solvents or rea...

Страница 18: ...te on the back of the instrument CE mark for European Conformity Type plate on the back of the instrument CSA listing mark for Canada and the USA Type plate on the back of the instrument Legal manufac...

Страница 19: ...11 1 9 Symbol Location Description Type plate on the back of the instrument RoHS mark for China the restriction of the use of certain hazardous substances in electrical and electronic equipment Back o...

Страница 20: ...Safety Information 1 10 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 21: ...in a safe condition 2 1 About this user manual This user manual provides information about the QIAgility in the following sections 1 Safety Information 2 Introduction 3 General Description 4 Installa...

Страница 22: ...erienced scientists with extensive practical and theoretical expertise in sample and assay technologies and the use of QIAGEN products If you have any questions or experience any difficulties regardin...

Страница 23: ...l version 3 0 2 3 Intended use of the QIAgility The QIAgility is designed to perform automated PCR setup and liquid handling actions in molecular biology applications If used in combination with QIAGE...

Страница 24: ...requirements No special requirements Installation Laboratory technicians or equivalent Appropriately trained and experienced personnel familiar with use of computers and automation in general Routine...

Страница 25: ...ductive tips enables high precision pipetting 1 The QIAgility worktable is set up with a Master Mix Block a Reagent Block tips and plates tubes The instrument is then calibrated Note Tubes are placed...

Страница 26: ...lity User Manual 11 2011 3 2 The QIAgility Lid Pipetting head Robotic arm Feet Tip Rack Holder and tips Tip chute Tip Rack Holder and tips HEPA filter Master Mix Block and Reagent Block UV lamp 1 2 5...

Страница 27: ...robotic arm to pause Extreme care should always be taken when opening the instrument lid during a run as the pause in robotic operation may take several seconds to be completed The instrument lid may...

Страница 28: ...gility the tip chute is held in place by a locating block allowing easy placement and removal for cleaning Note Details on removing and replacing the tip chute tip ejector chute are described in Secti...

Страница 29: ...trument lid is securely closed For increased safety an electronic interlock ensures that the UV lamp operates only when the instrument lid is closed Under no circumstances should the interlock be disa...

Страница 30: ...uld always be fixed or locked before the QIAgility is moved 3 2 7 Worktable The worktable is the instrument surface upon which items such as plates reagents and tips are placed Worktable layout is spe...

Страница 31: ...ser Manual 11 2011 3 7 3 2 9 Accessory panel QIAgility accessory panel Accessory 1 USB socket Accessory 2 Accessory power outlet Accessory 3 Main power input socket Serial RS 232 socket Power switch 1...

Страница 32: ...0 Internal features of the QIAgility X axis Worktable Y axis Master Mix Block Z axis Reagent Block Pipetting head cover Tip Rack Holders and tips Pipetting head Y arm Pipetting hub Tip ejector 1 2 3 4...

Страница 33: ...head is located under the protective pipetting head cover The pipetting head consists of a motor driven backlash compensated pipetting mechanism This mechanism is similar to most hand pipets Y arm Th...

Страница 34: ...The QIAgility is supplied with 2 Master Mix Blocks which can hold 5 x 5 ml free standing tubes 1 x 5 ml free standing tubes and 4 x 1 5 ml tapered tubes Reagent Block The Reagent Block is intended to...

Страница 35: ...Tubes 1 x Adapter Flip Cap 32 x 1 5 ml Tapered Base The 96 well adapters are intended to hold strip tubes and unskirted and half skirted PCR plates The 32 well adapter enables caps to be locked in pos...

Страница 36: ...eactions and are normally supported by the 96 well adapters Important Tubes supplied by different manufacturers may have slightly different dimensions despite a similar appearance Such differences can...

Страница 37: ...e liquid inside a tube the smaller the volume of liquid inside the tube the lower the change in capacitance For correct functioning of the liquid level sensor it is essential that the plates that are...

Страница 38: ...is supplied as part of the QIAgility System However if a different computer is used to operate the QIAgility or to run the QIAgility Software then the following desktop or laptop hosting systems are...

Страница 39: ...ty PCMCIA computer converter RS 232 QIAgility Note The laptop that is supplied with the QIAgility supports RS 232 and USB connections We recommend that the QIAgility be connected to the laptop via the...

Страница 40: ...General Description 3 16 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 41: ...arefully A change in position necessitates a recalibration Place the QIAgility in its final position The requirements for the QIAgility location and position are given below The QIAgility must be loca...

Страница 42: ...that the QIAgility does not share a wall socket with other laboratory equipment and that the QIAgility is plugged directly into its own wall socket Important The wall socket should be easily accessibl...

Страница 43: ...er outlet that has no ground earth connection 4 4 Unpacking the QIAgility Before unpacking the QIAgility move the package to the site of installation and check that the arrows on the package point upw...

Страница 44: ...ng it Retain the packaging in case you need to transport or ship the QIAgility in the future Instructions for packing the QIAgility are outlined in Appendix H Using the original package minimizes dama...

Страница 45: ...dures QIAgility User Manual 11 2011 4 5 2 Move the pipetting head to the right and remove the transport lock from the worktable 3 Unscrew the Z axis locking screw When this screw is removed the pipett...

Страница 46: ...fixing into the UV plug Always wear safety gloves WARNING UV irradiation hazard W9 An electronic interlock ensures that the instrument lid must be closed for operation of the UV lamp Under no circumst...

Страница 47: ...y Software See Section 4 6 for detailed information about how to install the software Note Some virus protection software is known to interfere with QIAgility Software and will cause the software to r...

Страница 48: ...ill guide you through the installation Instructions are displayed on each screen of the wizard Note We recommend using the default settings for all screens 7 Click on the Next button to continue with...

Страница 49: ...Note If a previous software version is already installed the Select Destination Location screen will not appear The following message box will appear instead 10 To uninstall a previous software versio...

Страница 50: ...menu to select your region Click on the Next button to continue 12 Select the additional tasks and then click on the Next button Select Create a desktop icon to create a desktop icon to easily launch...

Страница 51: ...anual 11 2011 4 11 13 Review the displayed details Click on the Install button 14 The QIAgility Software will now be installed onto your computer This could take a few minutes 15 If you are using Wind...

Страница 52: ...y User Manual 11 2011 For correct installation of USB drivers click on the Continue Anyway button 16 If you are using Windows 7 the following message will appear For correct installation of USB driver...

Страница 53: ...n click on the Next button to continue with the software installation 18 To directly launch the QIAgility Software select Launch QIAgility and then click on the Finish button If you do not want to lau...

Страница 54: ...el The Control Panel window will open 2 Double click on the Add or Remove Programs icon 3 Select QIAgility from the list of installed programs and then click on the Remove button To uninstall the soft...

Страница 55: ...re saved in this subfolder Data SupportPack ages Diagnostic support packages are saved in this folder Help Contains a PDF of the QIAgility User Manual version 3 0 HTML Contains a HTML version of the r...

Страница 56: ...empt to modify or delete these folders 4 7 1 QProtocols folder Storing Q Protocols in this folder enables access to the Protocols tab in the splash screen that opens when the software starts and also...

Страница 57: ...ake both connections at the same time To connect via the RS 232 interface Connect the 9 pin serial cable between the RS 232 port on the instrument and the RS 232 port on computer To connect via the US...

Страница 58: ...led on the computer before the QIAgility is switched on see Section 4 5 4 Connect the QIAgility to the power outlet as follows 1 Ensure that the power switch of the QIAgility is set to the off positio...

Страница 59: ...ty User Manual 11 2011 4 19 1 Select No not this time and then click on the Next button 2 We recommend that you select Install the software automatically 3 Click on the Next button to continue 4 Click...

Страница 60: ...d 2 Set the power switch of the QIAgility to the on position and switch the computer on The pipetting head should move upwards If this does not happen please consult the Troubleshooting section of thi...

Страница 61: ...click Empty Project last used layout or Empty Project default layout 6 The following message appears 7 Click on the OK button to proceed to tip ejector calibration See Section 7 1 1 for more details...

Страница 62: ...Installation Procedures 4 22 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 63: ...Protocols can be downloaded from www qiagen com goto QIAgility Select the User Support tab and look under the Protocols header For convenience the software automatically calculates the required volum...

Страница 64: ...g operations on the instrument This is particularly useful for checking new run files In Virtual Mode all software functions are enabled Run files which are developed in Virtual Mode can be saved and...

Страница 65: ...or Software workspace Menu bar Mode indicator Toolbar Software workspace 5 3 1 Menu bar The menu bar contains 5 menus File Control Wizards Options and Help All software functions can be controlled fro...

Страница 66: ...options to create a new run file open a recently used run file or to open a Q Protocol Open Enables an existing run file to be opened Select the run file and then click on the Open button This file c...

Страница 67: ...protocol Unlocks the protocol which is currently loaded This option is only available when a Q Protocol or User Developed Protocol is loaded 1 8 Lists the 8 run files which have been used most recentl...

Страница 68: ...ction data or generate pre run reports PCR setup wizard Launches the PCR Setup Wizard This wizard can be used to create new run files See Section 5 10 1 for further details Optimisation wizard Launche...

Страница 69: ...Options menu The Options menu includes options to manage user groups manage calibration settings and to configure pipetting and plate options User management Provides options to create user groups an...

Страница 70: ...ange of storage devices e g USB stick See Section 5 5 1 Calibration management Load calibrations from setup disk Enables calibration settings to be loaded from a setup disk onto a computer See Section...

Страница 71: ...Enables selection of the COM port which is used for communication with the QIAgility See Section 5 11 Robot setup Enable UV light In Virtual Mode this option enables or disables a virtual UV lamp Whe...

Страница 72: ...ment of the pipetting speed i e the aspirate eject speed See Section 5 6 2 Run settings Use liquid level when pipetting Enables the method of tracking liquid level to be selected There are 3 options D...

Страница 73: ...clude calibration settings Run settings reset settings to factory default Resets software settings to the factory defaults Click on the Yes button to continue Run settings System colour configuration...

Страница 74: ...e See Section 5 7 1 Change accessory Launches the Select Accessory window which enables the user to add or remove an accessory from a selected position on the software worktable See Section 5 7 1 Chan...

Страница 75: ...formed since the last instrument service Explore save directory Launches a file Explorer window with the default file directory where run files are saved Display log file Displays the log file This fi...

Страница 76: ...ameters except tip availability are saved Select this option to start a run See Section 6 4 5 Pauses the instrument during a run A dialog box will appear Click on the Continue Run button to continue t...

Страница 77: ...HEPA filter is not installed this icon will be shown in gray See Section 6 3 Indicates a problem with the run Errors must be resolved and acknowledged before the run can continue Opens the Service Re...

Страница 78: ...uid level sensing option Tip reuse option User logged in Windows Plate buttons Right hand pane Software worktable The software worktable visually simulates the instrument worktable The 6 SBS positions...

Страница 79: ...indicates whether the liquid level sensor is enabled Double click on this bar to change between Tip sensor Estimate and Not Used Tip sensor Indicates that liquid level sensing is enabled Estimate Indi...

Страница 80: ...osition or height calibrated and will remain visible until all of the calibrations for that plate have been completed Warning Prompts the user to proceed to plate calibration Opens the Set Alignment d...

Страница 81: ...he right hand pane when Minimize or Restore has been selected from the same icon in the menu bar Left click on the icon to access the following options Restore Reduces the size of the right hand pane...

Страница 82: ...formation about the Reaction right hand pane Progress report Please refer to Section 5 9 8 for more detailed information about the Progress report right hand pane 5 4 User management User management a...

Страница 83: ...st If required standard Windows file system write protection mechanisms may be used to protect run file data 5 4 2 User classes The QIAgility Software supports the following user groups QIAgility Oper...

Страница 84: ...rs can Enable and disable the access control function in the software Enable User Access option see Section 5 4 3 page 5 23 Update group membership for each of the QIAgility groups if the Manager is a...

Страница 85: ...access control when this option is enabled If the access control is enabled it is directly active Be careful to enable the access control only if you are a Manager 5 4 4 Managing user groups If your...

Страница 86: ...011 4 Select the group to which the selected user should be assigned i e Administrators QIAgility Manager QIAgility Designer or QIAgility Operator in the Windows Groups panel 5 Click the button to mov...

Страница 87: ...or a pop up of the operating system will appear to ask for elevation 5 4 5 Change current user To change users the current user must shut down the software and log out of Windows The new user must sub...

Страница 88: ...re settings from one computer to another using a Setup Disk This is useful when the instrument is connected to a new computer The Setup Disk can be any storage device e g USB stick Note It is not nece...

Страница 89: ...utton to continue 3 A default filename and location will be supplied If desired these can be changed 4 Click on the Save button The current calibration settings are now saved on the Setup Disk and can...

Страница 90: ...trument has existing calibration settings the following dialog box will appear 4 Click on the Yes button to overwrite the existing calibration settings 5 5 3 Delete multiple calibrations To delete exi...

Страница 91: ...The default run settings are the recommended settings However in some circumstances it is useful to adjust the run settings The default run settings are as follows Level Sensor On Error Mode Interrup...

Страница 92: ...eactions Alternatively to return to the default air volume settings click on the Restore Defaults button Note Larger air volumes may be beneficial when pipetting viscous liquids Smaller air volumes ma...

Страница 93: ...normal Defines the aspirate eject speed when pipetting normal reagents Reagent speed viscous Defines the aspirate eject speed when pipetting reagents which have been defined as viscous Standards spee...

Страница 94: ...olume from their default settings can be useful when preparing master mix for manual PCR setup or when preparing master mix for PCR setup at a later time To change the master mix extra volume settings...

Страница 95: ...e 1 Select Set standard extra volume from Options Run settings 2 The Standards Extra Volume dialog box will open 3 Enter a new extra volume Minimum extra volume required Defines the additional volume...

Страница 96: ...ram microliter l dollars microliter Custom user defined 5 6 6 Allow use of multiple reagent volumes When activated a tick is visible If activated the user sees the advanced reagents table in the react...

Страница 97: ...s three subdirectories Logs Reports and SupportPackages See Section 4 7 for more details about these subfolders To create a new default file location 1 Select Options Run settings Set default file loc...

Страница 98: ...ple colors Liquid Colours Enables configuration of liquid reagent colors Selection Colours Enables configuration of colors that represent a liquid function and that represent members of a sample bank...

Страница 99: ...QIAgility Software QIAgility User Manual 11 2011 5 37 Liquid Colours Selection Colours Enables selection of a color Adds selected color...

Страница 100: ...options To adjust liquid level tracking options 1 Select Options Run settings Use liquid level when pipetting 2 Select 1 of the 3 options Do not use liquid level Detect liquid level using sensor or Us...

Страница 101: ...s essential that the plates that are physically located on the instrument worktable are correctly assigned in the software Note The Use liquid level estimate function relies on the assumption that sou...

Страница 102: ...the 3 options Interrupt run on all errors Interrupt run on serious error or Ignore all errors Interrupt run on all errors This is the default error level All liquid level tracking errors are reported...

Страница 103: ...tip will be used for each aspirate eject cycle This option gives the highest accuracy and precision but requires a large number of tips Run duration will be increased Re use tips where possible Tips w...

Страница 104: ...ured Note These options are disabled if a Q Protocol is loaded and locked 3 Select Use each tip for one operation only or select Re use tips where possible from the drop down menu 4 If tip reuse has b...

Страница 105: ...og box will open Use air on multi eject Select to blow out air on the final ejection This limits accumulation of liquid on the tip interior This is a default setting when tip reuse is selected but is...

Страница 106: ...itial ejection volume 7 Define the Additional Extra Volume Note Setting this value to zero may reduce precision It is recommended to set this volume to half the volume of the aliquot 5 7 Define workta...

Страница 107: ...Click to toggle between an enlarged view and a normal view of the current plate type This option has the same function as the Zoom plate button Delete Calibration data Deletes all calibration setting...

Страница 108: ...es which match the selection will be listed in the Plate panel Plate Lists plates that match the plate type selection in the Select Plate Type drop down menu Select a plate from this list to view an i...

Страница 109: ...ing order and horizontal label order the sequence in which wells are listed in the grid changes according to the well display order selection This function is useful for example when it is convenient...

Страница 110: ...ration dialog box will appear 4 Select a plate type from the Select Plate Type drop down menu The Plate list will be automatically updated 5 Select a plate from the Plate list Note If you cannot find...

Страница 111: ...ple bank this enables a reaction to be a component of a different reaction A reaction that is a component of another reaction is called an Intermediate reaction Standard A standard is a dilution serie...

Страница 112: ...Note Use of an accessory affects the plate height Plate height calibration must therefore be performed with the plate and accessory together To add or remove an accessory 1 Right click on the plate w...

Страница 113: ...The instrument performs pipetting operations for the plate segment using the liquid level estimate function See Section 3 3 Use default liquid level mode Tip Sensor The instrument performs pipetting...

Страница 114: ...Tip function to select all the tips within that plate Alternatively to select a group of tips left click on the plate and then drag Selected tips will be highlighted red Note To select individual tips...

Страница 115: ...or Unavailable repeat steps 1 3 for each group of tips 5 8 Managing samples 5 8 1 Managing sample banks A sample bank is a collection of wells on a given plate or plate segment that is grouped and tre...

Страница 116: ...Add first n wells on plate to sample bank Opens the Update Sample Bank dialog box and enables consecutive wells on a plate to be added to a new or existing sample bank Add all wells on plate to sample...

Страница 117: ...Set preload volume Enables the user to enter the volume of liquids which have been preloaded into reaction tubes This option is only available when a Reaction plate is selected Click on the OK button...

Страница 118: ...st can consist of more than one description e g A1 B2 C2 C6 Bank wells Click to view the existing wells in the sample bank Wells are highlighted red Specify concentration range Select to define a conc...

Страница 119: ...he Update Sample Bank dialog box will open 3 Select New Bank and enter a name for the new sample bank 4 If desired left click and drag on the plate schematic in the lower portion of the dialog box to...

Страница 120: ...pane Before setting up a reaction list all plate functions must be configured This can be done using the right hand pane The right hand pane refers to the area on the right hand side of the software w...

Страница 121: ...e worktable The Tip right hand pane will appear Tip right hand pane 5 9 2 Standards Standard dilutions can be prepared using the QIAgility or a premade dilution series can be used The default location...

Страница 122: ...a premade standard dilution series The Set Custom Conc button will become available Standard name Displays the default name of the standard dilution series If desired click in the box and enter a dif...

Страница 123: ...the standard is used in at least one reaction configured in the run Set Custom Conc Opens the Custom Concentration dialog box which enables the concentration of dilutions to be defined Click on a cell...

Страница 124: ...ncentration values are assumed to be entered in a single unit common to all values in the run The unit itself can be defined as needed for the application See section 5 6 5 Entering concentrations usi...

Страница 125: ...a dilution series with varying ratios is required the dilution ratios can be modified To do this click on a cell in the Standard Data table and enter a different dilution ratio Click on the OK tab to...

Страница 126: ...me plates have several segments and therefore have more than one option for the location of reagents Reagents can be added directly into reaction tubes or can be used to make up a master mix which can...

Страница 127: ...fault volume for all reagents Click on the OK button to continue Name Defines the name of the reagent Names of reagents must be unique within the run Def Vol Defines the volume of reagent to be added...

Страница 128: ...is 0 l the reagent can still be used in the master mix component selection but it will not be shown in the reaction configuration 4 When a reagent name is entered the associated well will appear oran...

Страница 129: ...of maximal 16 ingredients Note Commercially available master mixes are not premade master mixes because they require addition of primers A commercial master mix and the individual primers must be defi...

Страница 130: ...are if unused Sets the minimum volume of master mix to create if the master mix is not used in any reactions in the run This allows the user to create a master mix without assigning it to reactions It...

Страница 131: ...r with the liquid in the mixture No of times to mix at end Defines the number of times to mix after addition of the last reagent Configure Mix Opens the Master Mix Component Selection dialog box which...

Страница 132: ...ume for this mix How much reagent to manually insert per reaction Defines the volume of reagent that will be manually inserted into each reaction Default reaction volume Defines the default reaction v...

Страница 133: ...located in Location Defines the location of the reagent on the plate Name Defines the name of the reagent Use Defines whether the reagent will be used in the master mix Vol per Reaction Defines the vo...

Страница 134: ...d in the Reagent right hand pane To change these volumes left click on the corresponding cell in the Vol per Reaction and enter a new volume Click on the OK tab The diluent volume is automatically adj...

Страница 135: ...gent Insertion Order dialog box will appear Dn Moves the selected reagent down the list Up Moves the selected reagent up the list Close Closes the Master Mix Reagent Insertion Order dialog box 3 Left...

Страница 136: ...2 Select Use Master Mix and then select Use pre mixed Master Mix 3 Enter a name for the master mix in the Master Mix name field 4 Enter the Volume per sample and the Number of times to mix before use...

Страница 137: ...eady be in use by previously defined reagents If the name of the reagent used in the saved master mix setup is identical to reagents already set up these will automatically be allocated to those posit...

Страница 138: ...must be organized into groups called Sample Banks Each sample can be assigned a name an ID e g a barcode and a starting concentration This information can be imported from an external file e g an Exc...

Страница 139: ...mple right hand pane Delete Bank Deletes the selected sample bank Deleting a sample bank will not affect sample names sample IDs or concentrations Rename Bank Opens a dialog box in which the sample ba...

Страница 140: ...concentrations are reset to 0 select sample bank A sample bank can be selected from the drop down menu Well Display Order This determines the sequence in which wells are displayed in the sample data g...

Страница 141: ...ane displays a list of current Sample Banks Click on the New Bank or Edit Bank buttons to open the Update Sample Bank dialog box Sample banks can be managed via this dialog box See Section 5 8 1 for m...

Страница 142: ...ed on their position in the sample plate To change the sample name to input a sample ID or to input a starting concentration left click on a cell in the Sample Name Sample ID or Conc column Directly e...

Страница 143: ...action Configuration dialog box Enables a reaction to be edited Alternatively double click on a reaction to open the Reaction Configuration dialog box Copy Copies selected reaction Left click on a rea...

Страница 144: ...tinue Displays a summary of the selected reaction Up Dn Modifies the reaction order Left click on the reaction to be changed in the Reaction List Hold down the Shift key to select multiple reactions C...

Страница 145: ...tion Info dialog box Clear Clears any user assigned reaction IDs and concentration values Well Display Order This determines the sequence in which wells are displayed in the sample data grid i e Label...

Страница 146: ...cells Right click on the selection and select Assign ID s Set Concentrations Enables groups of reactions to be assigned the same value in the Conc column Left click and drag to select a group of cells...

Страница 147: ...so no value is displayed If desired the user can assign a concentration value to the reaction well User assigned concentrations are shown in blue This is useful for dilution of samples or stock wells...

Страница 148: ...s of liquid handling operations in which the source wells for a given operation correspond to the target wells constructed by a predecessor operation Reaction Configuration dialog box To open the Reac...

Страница 149: ...r to the reaction well Mixes and reagents in mix wells Defines the master mixes that will be added to the reaction All wells that have Mix function and that have been assigned a per reaction volume wi...

Страница 150: ...automatically set when the operation includes a master mix configured with default sample volume A sample volume of zero is permitted Total volume Defines the per reaction total volume The Total volu...

Страница 151: ...s more than 1 component in the reaction Use normalization Instructs the instrument to normalize sample concentrations Auto Adjust small Volumes Instructs the instrument to round up sample volumes that...

Страница 152: ...enter the number of times to mix 6 Select the components of the reaction Available master mixes are listed in the Mixes and reagents in mix wells panel and available unmixed reagents are listed in th...

Страница 153: ...al options in the reaction setup A number of Special options are available from the Reaction right hand pane To view these options left click on the Special button Note It is recommended that reaction...

Страница 154: ...tervention is required to continue the reaction setup Click on the OK button to continue Add fan on Turns the HEPA filter on Add fan off Turns the HEPA filter off Add sample bank pooling step Opens th...

Страница 155: ...tion list It can be useful to introduce a pause into the reaction list if an incubation time is required between pipetting operations Note Pauses are processed during sample processing only after mast...

Страница 156: ...will appear 3 Click on the Special button 4 Select Add pause until confirmation 5 The Configure Pause dialog box will appear 6 Enter the text to be confirmed by the user 7 Click on the OK button The...

Страница 157: ...ing the Add sample bank pooling step option with the default tip usage setting the instrument picks up a tip takes an aliquot from the first plate takes an aliquot from the second plate and then takes...

Страница 158: ...contamination may occur Re use tips Instructs the instrument to use a single tip to aspirate and eject each pooling liquid separately This allows pooling of component volumes each up to the largest t...

Страница 159: ...e added to the target reaction wells in a pooling step Volume Defines the volume of each sample in the sample bank to be added during a pooling step Add Adds the defined pooling step to the Samples li...

Страница 160: ...he Add button 13 When Multi pickup is selected it is recommended to select Air gap from the drop down menu in the Samples panel 14 Define the Volume to be added and then click on the Add button 15 Rep...

Страница 161: ...ct Settings Opens the MULTI EJECT dialog box which enables configuration of multi eject options See Section 5 6 12 for more details about how to configure these options Re use tips Instructs the instr...

Страница 162: ...ume is dependent on the settings 6 Select the Tip Ejection Mode Multi pickup Re use tips or Use new tips 7 If Multi eject was selected the Multi Eject Settings option will be available See Section 5 6...

Страница 163: ...on step This function performs dilutions of a bank of samples with a diluent reagent Each dilution in the series is specified by a pair of volumes that describe the amount of sample and amount of dilu...

Страница 164: ...ons Diluent Multi eject Settings Opens the MULTI EJECT dialog box which enables configuration of diluent multi eject options See Section 5 6 12 for more details about how to configure multi eject sett...

Страница 165: ...eted Sample volume Defines the volume of the sample bank that will be used in a dilution Diluent volume Defines the volume of diluent that will be used in a dilution Add Adds the dilution step to the...

Страница 166: ...and the Diluent volume and then click on the Add button 12 If required repeat steps 8 12 to configure more dilutions Note To remove a copy left click on a copy in the Reaction plates list and then cli...

Страница 167: ...le bank to be used in the serial dilution Opens the Plate well Selector screen which enables the target reaction plate to be selected First well Defines the first well of the reaction plate into which...

Страница 168: ...erial dilution from the drop down menus 8 Click on the button to select the target reaction plate The Plate well Selector window will appear 9 Left click on a reaction plate to select it The Serial Di...

Страница 169: ...normalized samples are prepared in separate reaction wells 1 Left click on a Reaction plate 2 The Reaction right hand pane will appear 3 Click on the Special button 4 Select Add sample bank normaliza...

Страница 170: ...lize to Mix count Defines the number of times to mix Use secondary target Instructs the software to prepare a copy of the normalized samples in a second target plate First well Defines the first well...

Страница 171: ...plate The Plate well Selector window will appear 8 Left click on a reaction plate to select it The Sample Normalization dialog box will reappear Note If there is only one Reaction plate this plate wil...

Страница 172: ...to be moved to waste Opens the Plate well Selector screen which enables selection of the target reaction plate Vessel Defines the vessel in the selected reaction plate to which waste will be moved Us...

Страница 173: ...use tips 11 Click on the OK button to return to the main software window The remove waste command will be added to the Reaction List Note With this function more than one sample will be added to one...

Страница 174: ...5 Select either New Bank or Existing Bank If New Bank is selected enter a sample bank name If Existing Bank is selected left click on a sample bank to select it 6 Click on the OK button to return to...

Страница 175: ...ator Comma is the default when selecting a CSV File Other separators e g tab can be used If needed the ASCII code for any character can be specified making the import function universal Tab The Tab ch...

Страница 176: ...maining options in the Import Options panel as desired and select the column from which this information will be imported Note The software will automatically limit the number of rows imported to prev...

Страница 177: ...the target plate or segment of the run During the import the wells to which data are imported are added to the selected sample bank Existing members of the sample bank prior to the import are retained...

Страница 178: ...ilter function allows the user to filter the imported data limiting the selection of rows prior to import During import only filtered rows presented in the Import Preview pane are included in the impo...

Страница 179: ...panel displays the samples to be imported Click the Import button to import the data The imported data will be displayed in the right hand pane Note No data is imported from a row in which all fields...

Страница 180: ...the Import button Repeat for each sample bank column in the file Exporting sample and reaction data Sample and reaction data which is listed in the Sample and Reaction right hand pane can be exported...

Страница 181: ...ation and a user selectable sample identifier which can be either sample name or sample ID Custom comma separated A csv text file with comma separators between columns The data can be user defined Cus...

Страница 182: ...according to special conventions which allow the identification of Detector Reporter Quencher entities required by the ABI SDS software See Appendix E ABI 7000 7500 SDS allelic discrimination A Setup...

Страница 183: ...ftware Concentration Option Determines the concentration value to be associated with the reaction for import into the Rotor Gene software Page Option Determines how the reaction data is to be grouped...

Страница 184: ...page created for and named after each master mix Reaction ID Reactions are segregated according to the reaction ID user assigned label associated with the well with 1 page created for and named after...

Страница 185: ...ment Relative to Segment The well numbers start with 1 at the beginning of the selected segment and proceed in the selected display order label or pipet within the segment Relative to Plate The well n...

Страница 186: ...abel e g G3 3 Sample type none unknown standard Sample type none unknown standard 4 Concentration if known Source concentration if known 5 Sample name Final contents including full names and volumes 7...

Страница 187: ...xport modes where the data can be freely defined the user must specify the data content by writing an export string During export these designators are substituted with actual corresponding data For e...

Страница 188: ...d The PCR Setup Wizard enables typical PCR reactions with samples standards and master mixes to be set up The wizard allows the user to set up reagents and then use these to prepare master mixes The w...

Страница 189: ...e of the screens will be used To set up a PCR reaction using the PCR Setup Wizard 1 Select Wizard PCR Setup Wizard or select PCR Setup Wizard from the splash screen that appears when the software is s...

Страница 190: ...n See Section 5 7 1 for more details about the available plate functions 4 Left click on a plate to select it Click on the Set Plate button 5 The Plate Configuration dialog box will open Change the pl...

Страница 191: ...in the Reagent column and updates all cells in the Viscous column to No 11 To change the reagent name left click on a cell in the Reagent column and then enter a new name To change a reagent from vis...

Страница 192: ...e OK button to continue Repeat this step until all reagent segments you want to use are defined 13 Click Next to continue 14 Left click on a well in the Master Mix Block to select the location of the...

Страница 193: ...gure the components of the master mix as outlined in Section 5 9 4 16 Click on the OK button to continue 17 The Standards Editor screen will appear Steps 17 to 21 will only appear if you selected I wa...

Страница 194: ...ill appear Number of dilution concentrations Defines the total number of wells in the dilution series including the initial undiluted stock well of the standard Concentration of undiluted standard Def...

Страница 195: ...ank name can be changed Click on the OK button to continue New Bank Displays the Update Sample Bank dialog box which enables a new sample bank to be created See Section 5 8 1 Edit Bank Displays the Up...

Страница 196: ...mix Defines the master mix that will be used in a reaction Standards samples Defines the standard or sample that will be used in a reaction iteration s Defines the number of reaction replicates that...

Страница 197: ...ard or sample from the second drop down menu and define the number of replicates in the field below 29 Click on the button to define the Target plate 30 Click on the Add button The reaction will now b...

Страница 198: ...screens that will guide the user through the setup Instructions are displayed on each screen Note The Optimisation Wizard only provides steps to set up an optimized matrix it does not provide steps fo...

Страница 199: ...oncentrations would you like to use Defines the number of different concentrations of reagent to be optimized Set Locations Opens the Reagent selector window which enables selection of the location of...

Страница 200: ...ocations do not matter click and drag the Auto white box to the desired reagent segment Reagents will automatically be assigned to a location Selected wells will be highlighted red Note Some reagent l...

Страница 201: ...lected in step 3 repeat steps 7 13 and then proceed to step 15 15 The Optimisation Wizard Replicates dialog box will appear How many replicates would you like to produce Defines the number of replicat...

Страница 202: ...en this option is not selected the wizard appends the optimization reactions to the existing reaction list 16 Define the number of replicates for each reaction 17 Define the master mix to be used in t...

Страница 203: ...pear when starting the software and can also be accessed by selecting File New 2 Select either Empty Project last used layout to use the layout from the most recent run or Empty Project default layout...

Страница 204: ...n list See Section 5 9 6 for details about how to do this In addition see Section 5 10 4 for examples of how to set up typical reactions 8 To save the run file select File Save As Enter a name for the...

Страница 205: ...Enter the number of replicates in the Target wells panel 4 Click on the OK button Master mix with sample bank 1 Select the sample bank from the Samples panel 2 Select the master mix 3 Enter the numbe...

Страница 206: ...wells panel 5 Click on the OK button No template control with master mix 1 Select Diluent from the Samples panel 2 Select the master mix 3 Enter the number of replicates in the Target wells panel 4 Cl...

Страница 207: ...ighted 5 10 6 Required volumes for reaction setup The software automatically calculates the volume of standard diluent reagents and master mix required for a reaction setup To view the volume of liqui...

Страница 208: ...vice register describes the current status of maintenance alert notification records that are associated with the instrument The software maintains a schedule of required service activities in the ser...

Страница 209: ...ervice at a future date Component Defines the selected component to be serviced Pipettor or Hepa Filter Due Date Defines the date that a service is required Acknowledg ment Date Displays the current d...

Страница 210: ...cknowledgment 7 The service that has been acknowledged will now be colored black in the Service Register dialog box In addition the component will be colored in green as a reminder of a future service...

Страница 211: ...QIAgility Software QIAgility User Manual 11 2011 5 149...

Страница 212: ...QIAgility Software 5 150 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 213: ...the current movement which may take up to 10 seconds WARNING CAUTION Risk of personal injury and material damage W4 Do not attempt to move the QIAgility during operation WARNING Moving parts W12 To av...

Страница 214: ...ures 6 2 QIAgility User Manual 11 2011 computer or if the instrument is switched off the software will run in Virtual Mode See Section 5 2 for more details about Virtual Mode operation 3 The welcome d...

Страница 215: ...e displayed left click on a run file to select it and then click on the Open button Alternatively double click on the run file to open it directly 4 The main software window will open See Section 5 3...

Страница 216: ...tes using the arrows Start Initiates UV lamp operation Close software when UV finishes Shuts down the QIAgility Software when UV lamp operation is complete Enable Lamp Switches the UV lamp on Disable...

Страница 217: ...will appear green 3 To switch the HEPA filter off click on the icon in the toolbar 6 4 Executing a run 6 4 1 Q Protocols Q Protocols are run files which have been developed to directly facilitate spec...

Страница 218: ...If the user attempts to overwrite an existing Q Protocol the following message will appear Using a Q Protocol 1 Start the QIAgility software as outlined in Section 5 1 Note If the QIAgility software...

Страница 219: ...nual 11 2011 6 7 4 Select the Protocols tab All Q Protocols that are saved in the appropriate folder i e QProtocols will be displayed 5 Select the desired Q Protocol 6 Click on the Open button 7 A des...

Страница 220: ...Section 5 7 2 modify sample banks see Section 5 8 1 assign plate IDs barcodes see Sections 5 9 5 5 9 6 and 5 9 7 and assign well IDs barcodes to sample reaction wells see Sections 5 9 5 5 9 6 and 5 9...

Страница 221: ...u 1 Select File from the menu bar Recently used run files are listed in the File menu 2 Select one of these files 6 4 3 Creating a new run file There are 2 wizards PCR Setup Wizard and Optimisation Wi...

Страница 222: ...uids that are required on the worktable for completion of the loaded run file To view the pre run report select Wizards Generate report See Section 6 7 1 for more details about the pre run report Note...

Страница 223: ...the cursor over the source well A hover box will appear which displays the required volume of liquid that the software has calculated This volume includes the extra volume which allows for liquid rec...

Страница 224: ...ort Check the worktable setup 6 Click on the OK button to start the run 7 The location of the pipetting head will be highlighted on the software worktable in real time and a summary of the progress of...

Страница 225: ...aborted run cannot be restarted from the point at which it was aborted 6 6 Exception handling If an exception i e an error or warning occurs during a run an alarm will sound for 60 seconds and then at...

Страница 226: ...ps 1 Add the correct tips to one of the available tip locations on the worktable 2 Select the Tip plate where the tips have been replaced by checking the box next to the correct plate 3 Click on the O...

Страница 227: ...a new e mail message which enables the pre run report to be e mailed Saves the pre run report as a Word document Assign a file name select a location and then click on the Save button Closes the pre r...

Страница 228: ...re Run Report button The pre run report will be displayed 6 7 2 Post run report Upon completion of a run a Post run report will appear This report contains the same information as the Pre run report b...

Страница 229: ...rectory called Reports in the current run file save directory The default directory is C Program Files QIAgility Data 6 7 3 Log files and support packages Log files A new log file is generated when th...

Страница 230: ...blems that occur outside the context of a run it is important to manually create the support package immediately after a suspected error has occurred Support packages are important when reporting a pr...

Страница 231: ...eption User paused job Displays all support packages in which the user paused the run file User aborted job Displays all support packages in which the user aborted the run file 3 The support packages...

Страница 232: ...top right corner of the screen or select File Exit to shut down the software 2 If a run is still active the software will display a warning message 3 Select Return to Run if you want to cancel the sh...

Страница 233: ...Position recommended was selected the robot will discard the tip and the pipetting head will now move to a safe location The following message will appear 13 Click on the OK button 14 Set the power s...

Страница 234: ...mmediately This is a faster way of shutting down The instrument axes remain engaged and the pipetting head remains in an upward position If the head holds a tip it will not be discarded As the instrum...

Страница 235: ...curate there is no need to recalibrate the volume Note If pipetted volumes are found to be inaccurate please contact QIAGEN Technical Services 7 1 1 Setup tip ejector Before starting ensure that the w...

Страница 236: ...red 7 Click on the Yes button The automated calibration procedure will start 8 Once the X and Y axis positions have been located the following message will appear 9 Check the pipetting hub position If...

Страница 237: ...utton The following dialog box will appear 11 Manually adjust the X and Y axis positions using the arrows and then click on the Locate Z Axis button 12 The following message box will appear 13 Click o...

Страница 238: ...jector calibration 7 1 2 Calibrate plate positions Before using a plate on the worktable a position calibration must be performed Plate position calibrations can be saved and reused Important Tip Rack...

Страница 239: ...d on the X axis During calibration the objective is to set this to match the center position of the calibration well Y position Indicates the position of the pipetting head on the Y axis During calibr...

Страница 240: ...ove the pipetting head so that it is just above the plate surface Ensure that the tip is not in contact with the plate Note If there is no tip attached the pipetting head can only move to a limited Z...

Страница 241: ...l tips are available that the Tip Rack Holder is calibrated and that the correct tips are set as available Ensure that empty tubes are placed in the first 4 wells of each plate 1 Select Options Robot...

Страница 242: ...tes Abort Aborts the current calibration activity Manual Setup highlighted items Initiates the manual plate height calibration operation Manual plate height calibration is performed only for the selec...

Страница 243: ...ating the calibration ensure that all tubes are sitting correctly and that the tips are not bent Note If there is significant variation across the 4 samples the following message box will appear Click...

Страница 244: ...the plate and bend the tip Raise tip to 0 Lifts the pipetting head to the current 0 position Lower tip to current setpoint Places the pipetting head at the height that corresponds to the displayed Lev...

Страница 245: ...the true distance of the base of the tip from the home position Use the Home Z axis button to reset the 0 position and repeat steps 5 7 starting with a lower Level at bottom of well value to avoid dr...

Страница 246: ...g the procedure Ensure that a plate with Reaction or Mix function with well sizes large enough to accommodate 50 l and 200 l tips is available this plate must be position and height calibrated Place a...

Страница 247: ...hich tip offset calibration will be performed Select tip types to profile Lists the tip types to be calibrated 200 l conductive tips are selected by default Test how many tips per tip type Defines the...

Страница 248: ...the average offset 7 Click on the Start button to start tip offset calibration 7 1 6 Calibrate volumes There is a direct relationship between the rotation of the motor shaft and the vertical movement...

Страница 249: ...an Empty Project last used layout or Empty Project default layout as outlined in steps 1 2 of Section 5 10 3 2 Select Options Robot Setup Calibrate Volumes 3 The Volume Calibration Wizard dialog box w...

Страница 250: ...ng the P axis value increases the volume aspirated or dispensed Conversely decreasing the P axis value decreases the volume aspirated or dispensed 1 Select Option 1 from the Volume Calibration Wizard...

Страница 251: ...played settings as new calibration Updates the default volume calibration with the displayed calibration settings 8 Current Settings are displayed by default Saved calibration settings up to 9 can be...

Страница 252: ...bration Wizard History of Calibration Data dialog box 12 Click on the Finish button to return to the main software window Option 2 Use Option 2 to view current calibration settings and to replace the...

Страница 253: ...tings Current calibrations are automatically saved in the volume calibration history 1 Select Option 3 from the Volume Calibration dialog box 2 The following message will appear 3 Click on the Yes but...

Страница 254: ...Instrument Setup and Calibration 7 20 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 255: ...this user manual Servicing The QIAgility is supplied with a warranty that lasts for 1 year beginning on the date of shipment The warranty includes all repairs due to mechanical breakdown Application d...

Страница 256: ...e instrument lid Alcohol will damage the lid To clean the lid use distilled water only CAUTION Damage to the QIAgility side panels C6 Never clean the instrument side panels with alcohol or alcohol bas...

Страница 257: ...nts in TriGene Medichem International for more information see www medi mark co uk or one of the tested cleaning agents listed in Section 8 1 1 above for 15 30 min 3 Thoroughly rinse the components in...

Страница 258: ...ip ejector chute Removing the tip ejector chute 1 Open the lid 2 Grasp the chain connected to the tip ejector chute 3 Using the chain pull out the sliding security bolt 4 Remove the tip ejector tip ej...

Страница 259: ...ress the sliding security bolt into the side slot opening 8 2 3 Replacing the HEPA filter 1 Lift the HEPA filter lid section 2 Remove the lid 3 Unscrew the 4 thumb screws 4 Remove the H Frame 5 Remove...

Страница 260: ...Maintenance Procedures 8 6 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 261: ...he computer and the instrument Check the connection of the USB or RS 232 cable See Section 4 5 b Incorrect COM port settings Check the COM port setting If the instrument is connected to the incorrect...

Страница 262: ...en configured properly Tip reuse causes greater liquid losses on the insides of tips due to capillary and surface tension effects Disable tip reuse Master mix may be viscous and build up on the outsid...

Страница 263: ...Screen savers and power save modes not disabled on the computer Disable screen savers and power save modes on the computer Z axis not rising after power has been switched on a Power cords not securely...

Страница 264: ...Incorrect tip configuration The instrument may have been configured to pick up tips that were not present in the Tip Rack Holder Ensure that tip configuration is correct b Incorrect plate height calib...

Страница 265: ...sage is received when the software is started Correct plate definitions not installed Uninstall and then reinstall the software See Section 4 6 Software does not start and the A required library file...

Страница 266: ...age describing the error or warning The run can commence with active warning messages but this may lead to erroneous results Note Warnings and errors are logged in the post run report and in any suppo...

Страница 267: ...s lower than the extra volume recommended to avoid shortages Standards volume too high An instrument prepared standard well has a volume that is too large for a 200 l tip to mix effectively Speed not...

Страница 268: ...equired volume are not available Volume calibration must be done before run Volume calibration must be done before a run can be started in these situations New installation of instrument After pipetto...

Страница 269: ...OM 129 An invalid axis was specified 130 The instrument did not respond with the first reply 131 The instrument did not respond with a second reply 132 The COM port has problems 133 The micro firmware...

Страница 270: ...147 A bad packet length was received 148 More data was received while processing 9 4 Self diagnostic tools These tests are designed to collect information to help with troubleshooting Note These test...

Страница 271: ...e Pipettor Head See Section 9 4 1 for more details Check Commun ications See Section 9 4 2 for more details Liquid Sensor Test See Section 9 4 2 for more details Close Closes the dialog box Go Home Ho...

Страница 272: ...about how to save a support package Cycle Pipettor Head After calibration or if the instrument has been unused for a long period of time it is recommended to cycle the pipetting head This helps to dis...

Страница 273: ...11 9 13 appears during the test there is no problem with the communications links Liquid Sensor Test This test checks that the liquid sensor circuitry is functioning correctly so that it only triggers...

Страница 274: ...Troubleshooting 9 14 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 275: ...t volume of the reaction Master Mix Block The Master Mix Block is intended to hold diluent and master mix on the instrument worktable Menu bar The menu bar contains 5 menus File Control Wizards Option...

Страница 276: ...ht side of the software workspace It provides access to options which relate to different plate functions When a run is in progress a progress report is displayed in the right hand pane Run file A run...

Страница 277: ...e pipetting head is highlighted on the software worktable Standard A standard is a dilution series with known concentrations Standards are treated like samples Virtual Mode Virtual Mode enables run fi...

Страница 278: ...Glossary 10 4 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 279: ...ctuations are not to exceed 10 of the nominal supply voltages Fuse F5A 250 V fuse Overvoltage category II Air temperature 15 30 C 59 86 F Relative humidity 40 70 Altitude Up to 2000 m 6500 ft Place of...

Страница 280: ...mensions Width 54 cm 21 3 in Depth 63 cm 24 8 in without cables 72 cm 28 3 in with cables Height 45 cm 17 7 in lid closed 86 cm 33 9 in lid open Mass 41 kg 90 2 lb standard configuration Capacity Up t...

Страница 281: ...f life The separate collection and recycling of waste electronic equipment at the time of disposal helps to conserve natural resources and ensures that the product is recycled in a manner that protect...

Страница 282: ...ply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is oper...

Страница 283: ...responsibility that the product QIAgility meets all applicable requirements of the following European Directives Low Voltage Directive LVD Electromagnetic Compatibility Directive EMC 2006 95 EC 2004 1...

Страница 284: ...Appendix A A 6 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 285: ...for the duration of the original warranty period and in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices inte...

Страница 286: ...Appendix B B 2 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 287: ...s de s curit suivants sont fournis tout au long du manuel AVERTISSEMENT Le terme AVERTISSEMENT signale des situations risquant d entra ner des accidents corporels dont l utilisateur ou d autres person...

Страница 288: ...GEN AVERTISSEMENT ATTENTION Risque de surchauffe W2 Afin de garantir une bonne ventilation laisser un d gagement d au moins 10 cm sur les c t s et l arri re du QIAgility Les fentes et les ouvertures q...

Страница 289: ...il et ab mer les l ments en plastique Tenir le QIAgility l abri de la lumi re directe du soleil ATTENTION D t rioration de l appareil C2 viter de renverser de l eau ou des produits chimiques sur le QI...

Страница 290: ...sous tension AVERTISSEMENT Danger d irradiation UV W9 Un dispositif lectronique de verrouillage emp che la lampe UV de fonctionner tant que le capot de l appareil est ouvert Ce dispositif ne doit en a...

Страница 291: ...ologique Les sp cimens et les r actifs contenant des mati res provenant d tres humains doivent tre consid r s comme potentiellement infectieux Utilisez des proc dures de laboratoire s res comme d crit...

Страница 292: ...convenablement form s et ne sont pas expos s des niveaux dangereux d agents infectieux comme cela est d fini dans les fiches techniques sant s curit MSDS ou dans les documents de l OSHA de l ACGIH ou...

Страница 293: ...ise au rebut des d chets doivent s effectuer conform ment toutes les r glementations et lois nationales r gionales et locales relatives la sant et la s curit OSHA Occupational Safety and Health Admini...

Страница 294: ...de mani re satisfaisante et en toute s curit suivre les conseils suivants Utiliser uniquement les tips et les supports de tips recommand s Dans la mesure du possible maintenir le capot de l appareil f...

Страница 295: ...lity Risque d accident corporel et de d t rioration du mat riel Effectuer uniquement la maintenance sp cifiquement d crite dans ce manuel ATTENTION D t rioration de l appareil C4 Ne pas utiliser de so...

Страница 296: ...le de danger biologique Table de travail Symbole de risque d crasement Plaque signal tique l arri re de l appareil Marque CE Plaque signal tique l arri re de l appareil Label CSA pour le Canada et les...

Страница 297: ...appareil Label C Tick pour l Australie identifiant du fournisseur N17965 Plaque signal tique l arri re de l appareil Label RoHS pour la Chine restriction de l utilisation de certaines substances dang...

Страница 298: ...Appendix C C 12 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 299: ...genden Kategorien von Sicherheitshinweisen verwendet WARNUNG Der Begriff WARNUNG WARNING weist Sie auf Situationen hin in denen eine Verletzungsgefahr f r Sie selbst oder andere Personen besteht N her...

Страница 300: ...R ckseite und Seitenteile ein Mindestabstand von 10 cm eingehalten wird Das gew hrleistet eine ausreichende Bel ftung des Ger tes Die L ftungsschlitze und ffnungen die f r die Be und Entl ftung des QI...

Страница 301: ...hemikalien auf der Oberfl che des QIAgility zu versch tten Durch versch ttete Chemikalien oder versch ttetes Wasser verursachte Ger tesch den sind nicht durch die Garantie abgedeckt WARNUNG Verletzung...

Страница 302: ...lektronisch gesteuerte Verriegelung stellt sicher dass die Ger tehaube geschlossen sein muss bevor die UV Lampe eingeschaltet werden kann Diese Verriegelung sollte auf keinen Fall deaktiviert werden U...

Страница 303: ...g A angegeben gelagert wurde das Ger t unsachgem transportiert worden ist 1 3 Biologische Sicherheit Bei Substanzen und Reagenzien die humanes Untersuchungsmaterial enthalten sollte immer von einer m...

Страница 304: ...Arbeits platzes sicher ist und die Bediener des Ger ts ausreichend geschult sind Au erdem d rfen die Grenzwerte in Bezug auf infekti se Erreger die in den entsprechenden Sicherheitsdatenbl ttern MSDS...

Страница 305: ...Beim Betrieb des Abzugs und bei der Entsorgung von Abfallstoffen m ssen alle Bestimmungen und Gesetze zu Gesundheitsschutz und Sicherheit am Arbeitsplatz auf bernationaler nationaler und regionaler Eb...

Страница 306: ...dringend einen Neustart des Systems durchzuf hren falls der Y Arm versehentlich von Hand bewegt wurde Um einen zufriedenstellenden und sicheren Betrieb des QIAgility zu gew hrleisten befolgen Sie bitt...

Страница 307: ...eiten gem den Anweisungen in Abschnitt durch QIAGEN stellt alle Reparaturen in Rechnung die nachweislich auf eine inkorrekte Wartung zur ckzuf hren sind WARNUNG ACHTUNG Verletzungsgefahr und Besch dig...

Страница 308: ...eagenzien die S uren Laugen oder Abrasivstoffe enthalten um die QIAgility Haube zu reinigen Alkohol sch digt die Ger tehaube Verwenden Sie zum Reinigen der Ger tehaube ausschlie lich destilliertes Was...

Страница 309: ...der R ckseite des Instruments CE Kennzeichnung Typenschild auf der R ckseite des Instruments Symbol der CSA Zertifizierung in Kanada und den USA Typenschild auf der R ckseite des Instruments Herstell...

Страница 310: ...truments C Tick Mark Zeichen f r Australien Herstellerkennung N17965 Typenschild R ckseite des Instruments Markierung gem RoHS Richtlinie f r China Beschr nkung der Verwendung bestimmter gef hrlicher...

Страница 311: ...ing of characters which excludes Is a separator D Is a placeholder for detector R Is a placeholder for reporter Q Is a placeholder for quencher Indicates an optional component Indicates 0 or more item...

Страница 312: ...tector for CMV using FAM reporter and TAMRA quencher and detector for EBV using JOE reporter and TAMRA quencher Mix D represents the marker name in the SDS software The allelic discrimination function...

Страница 313: ...ng Three Stage Instructs the instrument to collect tips in 3 separate downward movements 1 The pipetting head is brought into the tip collar 2 The pipetting head is gently pressed against the tip coll...

Страница 314: ...dress a variety of situations where the tip would otherwise be placed too high in the well for pipetting operations Note Using this function may increase the amount of liquid carried on the exterior o...

Страница 315: ...s reaction wells sequentially from the first available well in the target plate not used by previous reaction setup operations in the run When activated the software attempts to use the well index in...

Страница 316: ...well while aspirating thereby approximately tracking the liquid surface during the operation By default this function is not activated Retract Z axis whilst dispensing When activated a tick is visibl...

Страница 317: ...ly causes insufficient volume to be aspirated and hence a deficiency in volume transferred to the target well This function presents options to reduce the impact of false triggering caused by static e...

Страница 318: ...ed tube well height more closely to the true internal base of the vessel Details are described below While this may reduce the wasted dead volume it also increases the risk that liquid may not be foun...

Страница 319: ...list Clear All Deselects all of the plates in the Plates to Calibrate list Require confirmation from user Instructs the software to present a message to the user after automatic plate height calibrati...

Страница 320: ...rate list Alternatively click on the Select All button to select all of the plates Note Plates which have multiple segments e g Reagent Blocks are listed more than once but with different tube types 4...

Страница 321: ...for each vessel to be measured Where the segment is not eligible for optimal height calibration a single tip is used to probe the base of the vessel and the process proceeds as described in Section 7...

Страница 322: ...termined height calibration value is automatically saved 10 If more than one plate was selected in the Plates to Calibrate list the instrument will calibrate each plate in turn Repeat steps 8 and 9 fo...

Страница 323: ...abel WellID WellNo 1 WellNo StandardWellName A 1 StandardWellName Color 5775359 Color TubeType BioSprint_250 TubeType Concentration 37 Concentration FinalWellVol 80 FinalWellVol EventOccurred 0 EventO...

Страница 324: ...g Create sample banks from target wells As the XML export can be used to export more than one plate the Socket tag might be repeated for every exported plate data It is possible to create XSL transfor...

Страница 325: ...Appendix G QIAgility User Manual 11 2011 G 3 EmptyLabelType Possible values EL_Empty 0 EL_WellID 1 EL_WellOrder 2 EL_WellNumber 3 They can be evaluated during the transformation...

Страница 326: ...Appendix G G 4 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 327: ...the QIAgility package the instrument as follows 1 Open the hood and remove all plates and tips from the worktable 2 Shut down the QIAgility Software and switch off the QIAgility as outlined in Sectio...

Страница 328: ...Appendix H H 2 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 329: ...er 3 14 connecting 4 17 Conductive Filtered Tips 3 12 D Decontamination 8 3 Dilution series 5 63 E Errors 6 13 9 6 error codes 9 8 Exporting data 5 111 F Feet 3 3 Fumes 1 5 G Generic XML Format G 1 Gl...

Страница 330: ...otocols folder 4 16 QIAgility hardware features A 2 internal features 3 8 mechanical data A 2 R Reagent Block 3 10 Right hand pane mix 5 66 progress report 5 126 reaction 5 80 reagent 5 64 sample 5 76...

Страница 331: ...ailability 5 52 tip usage 5 41 toolbar 5 13 uninstalling 4 14 virtual mode 5 2 Storage conditions A 1 Support packages 6 18 Symbols 1 8 T Technical assistance 2 2 Tip chute 3 4 8 4 Tip disposal box 3...

Страница 332: ...Index Index 4 QIAgility User Manual 11 2011 This page intentionally left blank...

Страница 333: ...echnical 0800 914413 France n Orders 01 60 920 926 n Fax 01 60 920 925 n Technical 01 60 920 930 n Offers 01 60 920 928 Germany n Orders 02103 29 12000 n Fax 02103 29 22000 n Technical 02103 29 12400...

Страница 334: ...Technical 0800 914413 France n Orders 01 60 920 926 n Fax 01 60 920 925 n Technical 01 60 920 930 n Offers 01 60 920 928 Germany n Orders 02103 29 12000 n Fax 02103 29 22000 n Technical 02103 29 12400...

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