PerkinElmer OilExpress System, Руководство пользователя

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Routine Maintenance and Troubleshooting . 87
Maintenance of the Flowcell
In normal use of the OilExpress System, the cleaning utilities provided by the software
ensure that the flowcell is routinely kept clean and free of contaminants.
If, however, the flowcell does become contaminated, it may be carefully dismantled for deep
cleaning using lens tissues and heptane, then re-assembled. Contamination of the flowcell
may be detected during calibration (see
Troubleshooting
on page 93).
Routine Cleaning of the Flowcell, Using Software
Utilities
The procedure described below may provide an effective method for the routine cleaning of
your flowcell without the need to disassemble it.
The frequency with which you need to carry out routine cleaning will depend upon the
number and nature of the samples you work with. In particular, if you work with dirty or
sooty samples, you may find that you need to clean your flowcell more frequently than will
otherwise be the case.
1. With heptane in the solvent bottle, select
Autosampler Control
from the View menu
and then click
Wash
.
2. Repeat the heptane wash 10 times.
3. Place a mixture of toluene with 10% isopropyl alcohol in a sample bottle, then select
Autosampler Control
from the View menu and use the
Aspirate
function.
4. Aspirate the solution into the cell, allow it to settle for a few seconds, and then wash it
out with heptane, using the
Wash
function.
5. Repeat steps 3 and 4 a total of 10 times, then allow the solution to settle in the cell for
30 minutes before washing it out.
6. Repeat steps 3 and 4 a further 10 times.
7. Carry out 15 heptane washes.
8. Disconnect the tubing from the flowcell and dry it, either with air or by attaching it to a
vacuum pump.
9. Finally, carry out a Throughput check using
System Settings > Instrument Settings >
System Checks Setup
.