Medonic M16C-US, Руководство пользователя

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10.3 WBC Differentials
Description The Medonic M-Series uses a floating discriminator technology which
performs a mathematical calculation to estimate the best separation between 3
populations of white blood cells (lymphocytes, granulocytes and mid cell
fractions).
Floating
Discriminator
technology in
general After the analyzing process, the instrument finds two main modes
(Granulocyte Peak and Lymphocyte Peak) within the total distribution. By
extrapolating the two main population peaks value a third population can be
mathematically calculated. This third population is classified as MID cell area,
which mainly consists of monocytes. See Figure 10.2 below:
Figure 10.2
Differences in
technologies Some 3-part diff. technologies use a fixed discriminator analogue to separate
the 3 populations. However, as shown in the figure below, as a sample begins
to age, it can clearly be seen that the Granulocyte population is shifting
towards the Lymphocyte population. As the Granulocyte curve moves, the
accuracy of the results will decrease. Whereas, the floating discriminator
system is not dependent on the actual position of the two main populations
and thus overcomes this problem, and provides more accurate results.
Figure 10.3
LYMF
n
GRAN
MID cells
Volume (fl)
n
Fixed discriminators
4 hours old
Fresh” blood
GRAN
Volume (fl)
LYM