Minifors 2 - Benchtop Bioreactor
Cultivation
07 February 2019
Page
109
of
214
For details on operation, see the corresponding chapter in the main
chapter “Operation”.
INFORMATION
Depending on the specifications defined by the user, the pO
2
sensor is calibrated either before the vessel is filled with medium
or afterwards, in the prepared medium.
For more details on the calibration refer to main chapter “Opera-
tion”, chapter “Calibrating the pO
2
Sensor”.
9.2 Sampling
Samples are taken from the culture vessel to gain material for of-
fline analysis.
INFORMATION
Using
SAMPLE NOW
on the operating panel, the sampling can
be logged in the electronic logbook and assigned a sample ID.
For more details, see main chapter “Operation”.
The method of sampling can vary due to the different analyses car-
ried out by the operator.
The sampling procedure using the standard sampling system, Su-
per Safe Sampler, is described below.
Before starting, observe the following:
WARNING
Culture solution could emerge from the vessel if the sample
valve mechanically fails. This could lead to serious health risks
in the event of applications with pathogenic organisms.
– When working with pathogenic organisms, always addition-
ally clamp off the sampling hose with a metal (!) clamp.
– Only remove the clamp when sampling.
– Reattach the clamp before removing the syringe from the
sample valve.
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