Infors HT Minifors 2, Руководство по эксплуатации

Страница: 102 / 217

Minifors 2 - Benchtop Bioreactor
Before Cultivation
Page 100 of 214 07 February 2019
INFORMATION
Development of steam is not possible when autoclaving a com-
pletely empty and dry culture vessel. Successful sterilisation is
not guaranteed.
Ensure that there is liquid in the culture vessel (approx. 10 mL of
water per litre of total volume).

If necessary, pump off any remaining water after autoclaving
by means of the dip tube.

Sterilise all liquid, heat-instable components separately and
add them after autoclaving.

If the medium is autoclaved in the culture vessel, you may
then need to add sterile water to make up the volume.
When transporting the culture vessel to/from the autoclave, note
the following:

Always transport the culture vessel in the vessel holder.

Always transport the culture vessel to/from the autoclave in
pairs and use suitable auxiliary equipment when transporting
the culture vessel.
WARNING
Depending on the design, accessories and fill level, the culture
vessel may be too heavy to be carried by one person alone.
Proceed as follows to autoclave the culture vessel:
Procedure 1. Place the culture vessel into the autoclave.
2. Ensure that the culture vessel and the accessories do not
touch the inner wall of the autoclave.
3. Ensure that the exit gas filter is open.
4. Insert the temperature sensor of the autoclave into the pocket
for the temperature sensor.
5. Select the program for liquids.
6. Autoclave the culture vessel in accordance with the operating
manual of the autoclave manufacturer.