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P a g e
Step 1
Wavelength for this stored method is pre-set to 595
nm.
Step 2
Enter the number of standard concentration points
(1-9) to be used in the curve using the keypad
numbers or left and right arrows.
Press the down arrow key.
Step 3
Units: The user can enter a text string up to 8
characters long. To access a list of pre-defined
units press the Options key
and then use
the left/right arrows (µg/ml, µg/µl, pmol/µl, mg/dl,
mmol/l, µmol/l, g/l, mg/l, µg/l, U/l, %, ppm, ppb,
conc or none). These units can also be edited once
OK is pressed.
This screen also allows the number of displayed
decimal points (DP) to be selected, from 0 to 2
Note that the result will always be fixed to 5
significant figures regardless of how many decimal
points are selected (so 98768.2 will display as
98768 even with 1 decimal point selected).
Press OK
to store the chosen parameters
or Cancel
.
Step 4
Enter the type of curve fit. Options are: straight line
regression, zero regression (forces the straight line
through the origin), interpolated or cubic spline.
Press the down arrow key.
Bradford
The procedure is as follows: