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P a g e
Step 1
Wavelength for this stored method is pre-set to
562nm.
Step 2
Enter the number of standard concentration points (1-
9) to be used in the curve using the keypad numbers
or left and right arrows.
Press the down arrow key.
Step 3
Units: The user can enter a text string up to 8
characters long. To access a list of pre-defined units
press the Options key
and then use the
left/right arrows (µg/ml, µg/µl, pmol/µl, mg/dl, mmol/l,
µmol/l, g/l, mg/l, µg/l, U/l, %, ppm, ppb, conc or
none). These units can also be edited once OK is
pressed.
This screen also allows the number of displayed
decimal points (DP) to be selected, from 0 to 2 Note
that the result will always be fixed to 5 significant
figures regardless of how many decimal points are
selected (so 98768.2 will display as 98768 even with
1 decimal point selected).
Press OK
to store the chosen parameters or
Cancel
.
Step 4
Enter the type of curve fit. Options are straight line
regression, zero regression (forces the straight line
through the origin), interpolated or cubic spline.
Press the down arrow key.
Step 5
Select the calibration mode, either standards
(measure prepared standards), manual (keypad data
entry), or new standards (same as Standards but
forces new standards to be measured when the
method is re-run even if history is enabled and
standards have already been measured).
BCA
The procedure is as follows: