Media-Mate Plus Operation Manual
25-710-800-O
Volume Delivery Calibration
Calibration of volume delivery is recommended for all volume settings that will be used.
Recalibration is recommended every six months for each setting used. Recalibration is also
required whenever the delivery volume has been fine adjusted (see
Section 6 – Fine
Adjustment of Delivery Volume
). To facilitate volume calibration, Hanson Research
recommends the Volume Calibration Kit (p/n 25-710-705).
1.
Set Volume to desired increment (see
Section 7 – Volume Selection
).
2.
Hanson Research recommends performing volume calibration at room temperature. Set
temperature control to the desired temperature and allow the media temperature to
stabilize (see
Section 7 – Temperature Setting
).
3.
Place the Dispense Head over pre-numbered, plastic vessels (ref. p/n 25-710-705).
4.
Start the Media-Mate Plus cycle (see
Section 7 – Operating Instructions
).
5.
Using a calibrated laboratory scale, tare with empty vessel, weigh filled vessels, and
record the actual media weight of the delivered volume for each vessel.
6.
Empty and dry the vessels and repeat Steps 1 through 5 for a total of 5 cycles.
7.
Calculate and record the media volume for each vessel. The density of the media at the
calibration temperature is required for the calculation below.
Media Volume = media weight divided by media density at calibration temperature.
8.
Repeat all of the above steps for each incremental volume setting that will be used.
Fine Adjustment of the Delivery Volume
(one incremental setting only)
When dissolution rate calculations are corrected for actual mean delivery volumes recorded, it
should not be necessary to fine-adjust the delivery volume. For applications that do require a
more accurate delivery volume, adjust as follows:
1.
Calibrate volume at desired incremental setting (see
Section 6 – Volume Delivery
Calibration
).
2.
Review Calibration Data and identify adjusters that require finer adjustment.
3.
Fine adjustment is accomplished by accurately adjusting the Pointer position on the Fill
Tube (see
Figures 6-1 and 6-2
). The Pointer must be moved approximately 0.0048 in.
per mL of volume delivery change.
EXAMPLE:
To change delivery volume by 2.5 mL
[ 2.5 X 0.0048 = 0.012 ],
the Pointer
must be moved 0.012 in.
Section 6
Validation and Calibration
17
