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Genevac HT-8 & HT-12 Series II Evaporating Systems 

 
Page 60 of 

64  

04-4541

 

Issue 1-9 – June 2008 

 

Appendix A 

 
Programming Guide for Drying HPLC Fractions 
 

  

Drying HPLC Fractions 

Run number 

1 2 3 

  

  

  

  

Run Data Locked 

N N N 

Next Run Number 

2 3 0 

Run Name 

Fractions  

Fractions part 2 

Fractions part 3 

Sample Holder Type 

Your Holder 

Your Holder 

Your Holder 

Sample Volume ml 

Your Volume 

Your Volume 

Your Volume 

Solvent 

MeCN or MeOH & H

2

0  MeCN or MeOH & H

2

0  MeCN or MeOH & H

2

0 

  

  

  

  

SampleGuard Control Temp C 

40 40 40 

SampleGuard Control Channel 

1 1 1 

CoolHeat Enable Pressure 

50 50 50 

Chamber Temperature control 

preheat to 25 

preheat to 30 

preheat to 30 

Rotor Speed 

High High Low 

  

  

  

  

Heat-Off Option 

Elapsed Time 

Heat Flow 

Elapsed Time 

Heat-Off Elapsed Time 

ask for time 

- 

01:00 - 03:00 

End-of-Run Option 

Elapsed Time 

- 

Elapsed Time 

End-of-Run Elapsed Time 

ask for time 

- 

01:00 - 03:00 

  

  

  

  

Auto Defrost & Drain 

Y N Y 

  

  

  

  

Pressure Control Regime 

Variable Dri-Pure 

Controlled Pressure 

Full Vacuum 

Dri-Pure Start Pressure (mbar) 

175 - 

- 

Dri-Pure End Pressure (mbar) 

40 for MeCN 
45 for MeOH 

- 

- 

Dri-Pure Ramp Time 

20 -  - 

Final Control Pressure 

40 for MeCN      
45 for MeOH 

8 - 

 

 

 

 

Notes 

Remove organic phase Remove most of the 

aqueous phase 

Remove final 
“stubborn” solvents 

 

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Содержание HT-12

Страница 1: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

Страница 2: ...12 2 S Se er ri ie es s I II I E Ev va ap po or ra at ti io on n S Sy ys st te em ms s U Us se er r M Ma an nu ua al l Issue 1 9 June 2008 Part Number 04 4541 Artisan Technology Group Quality Instrum...

Страница 3: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 4: ...ample Holder on edge of swing 12 Series I vs Series II Swings 13 Balancing of swings and sample holders 13 Good procedural practice 14 4 3 Safe door operation 15 4 4 Limitations of use 15 5 Getting st...

Страница 5: ...f Conformity 54 11 Safety 54 12 Warranty Statement 55 13 Annex A 56 14 Useful information 64 These instructions are subject to change without notice No part of these instructions may be reproduced in...

Страница 6: ...sy to operate and very flexible The status of the system is displayed and controlled on a single keypad display module indicating the run time the vacuum rotor temperature and chamber temperature on d...

Страница 7: ...e of each symbol is as described below WARNING THIS SYMBOL INDICATES HAZARDS THAT CAN LEAD TO SERIOUS MATERIAL DAMAGE OR POTENTIAL SERIOUS INJURY Caution This symbol provides information about hazards...

Страница 8: ...vailable from drop down menu Lyophilisation Freeze Drying is available as a Rotor Speed Very Low 50g selection on evaporators equipped for this configuration Please note Lyophilisation samples are to...

Страница 9: ...ntact details 3 2 Arranging commissioning If your system is to be delivered separately Genevac Ltd will contact you prior to the delivery to agree a date to commission your system 3 3 Training Commiss...

Страница 10: ...ng pipe pipes fittings and power control lead included with it Connect your vacuum pump to the evaporator using the connecting pipe and flange fittings Connect the power and control lead between the p...

Страница 11: ...approximately Locate tubes correctly in tube holders Locate sample blocks correctly in sample swings Load two or four tube holders in opposite and balanced configurations Distribute tubes in sample ho...

Страница 12: ...ever not shown here the swings are generally fixed in place and cannot be lifted out Which swing is ideal depends on which sample holder is required There is a large range of sample holders which sit...

Страница 13: ...ist of the relevant weights Adherence to Mass Limit There is a maximum mass that can be loaded onto each position of a Genevac swung rotor this total includes The swing The sample holder The tubes or...

Страница 14: ...e it is necessary to make dummy samples to balance real loads the balancing load should be of a similar solvent composition For example do not balance 200g of 50 50 Water Acetonitrile with 200g of wat...

Страница 15: ...d and started by the same person Never leave system unevenly loaded and close the door Someone may start it Never start or restart a system without checking it is evenly loaded all sample holders are...

Страница 16: ...ser is not connected to the system 4 4 Limitations of use Your HT 8 HT 12 Series II evaporating system is unsuitable for use under the following circumstances With strong mineral acids such as HCl and...

Страница 17: ...itched on or when the Auto Stop function has been used 5 10 minutes for the CVP 5 2 How to switch on the evaporator Switch the mains switch on The screen will display the Software Control screen Press...

Страница 18: ...hen inputting or amending data Note that the START key also acts as an ENTER key when the keyboard is not illuminated Operating the MENU key displays the USER MENU screen only when the rotor is not sp...

Страница 19: ...t your runs on these screens Use the UP and DOWN cursor keys to move between profiles By moving across the horizontal menu bar You can START a run You can VIEW and EDIT a profile You can CLEAR a profi...

Страница 20: ...ENTER to open a field make the entry and press ENTER again to close the field During a run you access this screen by pressing the SETTINGS key You can edit any parameter during a run in this way Toggl...

Страница 21: ...new option to Auto Program the remaining run parameters Press Y to accept Auto Program If you have entered an unrecognised solvent by selecting Other from the solvent menu you will not be presented wi...

Страница 22: ...Run and continue If you did not select Auto Program or were not given the option due to inputting an unrecognised solvent then you will be presented with this screen If editing an existing run parame...

Страница 23: ...Sample Coolheat Enable Pressure Set within the range of 5 400 mbar Chamber Temperature Control There are now 3 options available Wait for Chamber to Heat Range from 0 C to 45 C Wait for Chamber to Coo...

Страница 24: ...ilable Heat Flow Automatically detects dryness by monitoring the rate at which the solvent is evaporating Low solvent loading or if the lamps are turned off may cause Heat Flow to fail If failure occu...

Страница 25: ...ms The block at the foot of the screen displays the current status of the system and Instructions Pressure is plotted on log scale Press STOP to terminate a run at any time Run finished press to view...

Страница 26: ...ENTER to start the defrost cycle This will automatically open the defrost valve Open the manual drain valve to drain the condenser when prompted Drain the condenser after every run and at the end of...

Страница 27: ...m down or N to select another run The SHUT DOWN screen will be displayed when you select the Y response The pump will be purged of solvent vapours for 30 minutes You can restart the run by pressing ST...

Страница 28: ...R Use the UP and DOWN cursor keys to adjust the screen brightness to suit your viewing angle Toggle the beep ON or OFF Press ESC to exit this screen 5 5 How to enter control data All control data is e...

Страница 29: ...ifferent steps Linked and looped runs can always be interrupted by manually stopping the system Use the DOWN cursor key to move to the next field Enter the title of the run that should be no more than...

Страница 30: ...normally be assigned to the probe in the sample holder and channel 2 to the probe in the sample Note that when powered down this setting defaults to channel 1 as the control channel Note this is not...

Страница 31: ...control the sample holder or swing temperature Either channel 1 or channel 2 may be used ensure that the channel chosen is selected as the control channel in the run options Automatically detects dryn...

Страница 32: ...e chamber pressure down to the vacuum capability of the pump When the Controlled Pressure option is selected the Controlled Pressure field will be displayed Move down to this field using the DOWN curs...

Страница 33: ...g channel 2 under these circumstances could result in damage to thermo labile samples Depending on the solvents being evaporated and the conditions significant cooling and or freezing will normally oc...

Страница 34: ...ll prompt you to confirm the change when you leave the Run Data screen Press Y for yes and N for no To verify the calibration of SampleGuard remove the Stuffer Plug shown connect the remote power lead...

Страница 35: ...r temperature is displayed on the condenser front panel and also on the RUN LOG screen refer to page 18 Note that due to sensor positions there may be a difference between the indicated temperatures o...

Страница 36: ...n to approximately 5 mbar pour approximately 100 ml of acetone into the funnel and open the flushing valve Always ensure that you close the flush valve after use Drain the vacuum pot and repeat as nec...

Страница 37: ...t condensation the chamber was preheated to 40 degrees centigrade Switch on the pump evaporator and condenser Display the Run Data screen and input the following data Run Data Locked No Next Run Numbe...

Страница 38: ...e or well and connect it to channel 2 on the SampleGuard refer also to additional notes on page 26 Always ensure that the tip of the sample probe is located at the bottom of the tube or well Close and...

Страница 39: ...dily as air is evacuated from the chamber and condenser With the Coolheat lamps operating from 100 mbar and because the chamber has been pre heated to 40 C the sample holders soon reach the pre set te...

Страница 40: ...ning traces of liquid DMF DMSO or NMP as this will contaminate the pump oil With the CVP pump check the oil level initially on a daily basis until a usage pattern has been established and then once we...

Страница 41: ...the chamber is not recommended when evaporating volatile solvents such as TFA acetonitrile or methanol TFA exhibits the property of creeping which is the movement of the TFA in liquid phase up the in...

Страница 42: ...sure of 6 mbar ice crystals are not formed and the time required for evaporation can be reduced by up to 30 Mixtures of water and methanol in deep well microtitre plates however respond entirely diffe...

Страница 43: ...es need be set the system will stop when all the samples are dry Each method has a venting procedure linked to the end of the evaporation process to help remove residual vapours from the chamber When...

Страница 44: ...precautions when using acetone in this manner Maintenance Regular inspection and maintenance of swings and sample holders should be performed at least monthly The following inspection routine is mand...

Страница 45: ...position the pump at the edge of a bench and connect the drain hose in the drain socket as shown Position a heat resistant plastic jug of at least 1 litre capacity under the drain hose and switch the...

Страница 46: ...however there will be some degree of wear and ageing of the seals and bearings in the chamber condenser and pump The extent of wear and ageing will depend on the utilisation of the system the severity...

Страница 47: ...r to discuss any ideas you may have please call Genevac Sales All sample holders are of a solid aluminium construction and black anodised to improve heat absorption Maximum contact area provide good p...

Страница 48: ...on has been made for the drainage of condenser solvents and the pump exhaust Ensure that the pump fluid is drained if the system is to be transported any appreciable distance CVP only Ensure that the...

Страница 49: ...tube from the chamber to the condenser port Connect the vacuum pump outlet to the condenser vacuum inlet port Connect Valve block to IN on the condenser exhaust pot Connect the T piece centre connecti...

Страница 50: ...ecessary Connect the heated inlet tube from the chamber to the condenser port Connect the vacuum pump outlet to the condenser vacuum inlet port Connect the pump exhaust to IN on the condenser exhaust...

Страница 51: ...ext Run Number Check that the condenser pot in use has been drained refer to section 5 8 How to use the condenser Check the sample holders and contents for weight differences of greater than 50g refer...

Страница 52: ...Service for assistance If the pressure is better than 1mbar reconnect the chamber and switch the pump on If the same conditions prevail contact Genevac Service for assistance Probable cause Contact G...

Страница 53: ...316 Stainless steel Condenser drain valve Stainless steel PTFE Stainless steel PTFE Vacuum system Pressure display resolution 0 1000 mbar 0 1000 mbar Vacuum control 0 1000 mbar 0 1000 mbar Dri Pure Y...

Страница 54: ...0A Power supply 2 1 5kW 230V 50Hz single phase 6 5A 208V 60Hz single phase 6 5A Environment The following figures apply Operating Ambient Temperature 15 C to 30 C Relative Humidity 10 60 Altitude Sea...

Страница 55: ...patibility EMC Limits Limits for harmonic current emissions equipment input current up to and including 16 A per phase BS EN 61000 3 3 1995 A1 A2 Electromagnetic compatibility EMC Limits Limitation of...

Страница 56: ...ed and operated in accordance with operator instruction manual 2 Unit only used for purpose it was sold and in accordance with Genevac published compatible solvent list 3 Regular cleaning and preventa...

Страница 57: ...several hours the system cannot achieve a lower pressure and so the higher boiling point solvents cannot be boiled The volatile solvents are therefore said to spoil the vacuum level Vacuum spoiling af...

Страница 58: ...hen to use Auto Defrost and Drain Auto Defrost and Drain has two main functions 1 to automatically drain the condenser mid process to eliminate volatile solvents enabling the system to reach full vacu...

Страница 59: ...of the stage this will be a short defrost just in case any small amounts of water have frozen in the drain port and will drain the organic solvent from the condenser allowing high vacuum levels to be...

Страница 60: ...ll you if your sample has frozen or not and when lyophilisation has ended Always fill your sample holders with samples when doing lyophilisation part filled holders may not freeze This method will not...

Страница 61: ...sure 50 50 50 Chamber Temperature control preheat to 25 preheat to 30 preheat to 30 Rotor Speed High High Low Heat Off Option Elapsed Time Heat Flow Elapsed Time Heat Off Elapsed Time ask for time 01...

Страница 62: ...CoolHeat Enable Pressure 50 50 50 50 Chamber Temperature control preheat to 25 preheat to 0 preheat to 0 preheat to 30 Rotor Speed High High Low Low Heat Off Option Elapsed Time Elapsed Time Elapsed T...

Страница 63: ...Temp C 40 40 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure 50 50 50 Chamber Temperature control preheat to 15 preheat to 40 preheat to 30 Rotor Speed High High Low Heat Off Option Elap...

Страница 64: ...Genevac HT 8 HT 12 Series II Evaporating Systems 04 4541 Issue 1 9 June 2008 Page 63 of 64 NOTES Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 65: ...ork 10989 United States of America Sales and Service Hotline 1 845 267 2211 Fax 1 845 267 2212 Email salesinfo genevacusa com 14 Useful information If you need to contact Genevac for assistance use ei...

Страница 66: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

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