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Action Step
In the Manual Instructions dialog: 5
For UV monitor U9-L:
• Select Monitors:Auto zero UV
• Click Execute.
For UV monitor U9-L, 2nd:
• Select Monitors:Auto zero UV 2nd
• Click Execute.
Result: The UV absorbance is set to zero.
Remove the syringe. 6
Repeat the injections with the remaining four test solutions in increasing concentration
order. Use a new syringe for each solution. 7
After each injection, wait for a stable absorbance value. Note the measured absorbance
values for each solution. 8
Enter the measured absorbance values into the table in the column UV-900 Absorbance
(AU).
Note:
The values should be converted from mAU to AU.
9
When all absorbance values have been entered into the table, the real UV flow cell path
length is shown at the bottom of the table.
Note:
The regression coefficient R2 should be larger than 0.999. If this is not the case, one or
more measured values are faulty.
10
Update the cell path length
Follow the instructions below to define the UV cell path length or the UV 2nd cell path length. The
flow cell path length should be updated when the flow cell has been replaced or calibrated.
Action Step
In the System Control module, select System:Calibration. 1
In the Calibration dialog, select 2
• UV cell path length
or
• UV 2nd cell path length
from the Monitor to calibrate drop-down list.
Enter the nominal flow cell path length in the Nominal length input field and click Set. 3
314 ÄKTA pure User Manual 29119969 AB
7 Maintenance
7.7 Calibration procedures
7.7.4 Calibrate the UV monitors