
Action
Step
In the System Settings dialog, select Conductivity and Cond temp compensation. Set
the Compensation factor to 0%, and click OK.
3
Wash the whole flow path and fill it with deionized water using a suitable inlet and the
System pump, until the conductivity value reaches 0.00 mS/cm.
4
Connect the tubing (labelled 3) from the system pump pressure monitor directly to the
conductivity cell inlet, by-passing the Mixer, Injection valve, Column valve and UV-monitor,
see image below. Ensure that the Flow restrictor is inline.
5
Prime and purge the inlet used in step 4 with the 1.00 M NaCl calibration solution. Fill the
conductivity cell with the calibration solution at 1 ml/min. Pump in at least 15 ml of the
calibration solution and wait until the conductivity signal and the temperature have
stabilized before continuing the calibration. See Section 5.4.1 System pumps, on page 162.
6
Under continuous flow, read the current Conductivity temperature from Run Data with
an accuracy of one decimal place.
7
In the Calibration dialog, select Conductivity monitor - user calibration from the Mon-
itor to calibrate drop-down list.
8
ÄKTA pure User Manual 29119969 AB
309
7 Maintenance
7.7 Calibration procedures
7.7.3 Calibrate the Conductivity monitor
Содержание AKTApure
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