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5.7.2 Sample application using a Superloop
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Introduction
A Superloop allows injection of large sample volumes onto the column. A Superloop can also be used
for multiple injections, for example in a scouting experiment when the same application conditions
are required. Superloop models are available in 10 ml, 50 ml and 150 ml sizes.
A superloop can be connected to either the Injection valve or the Loop valve. When using the Loop
valve, up to five loops can be connected simultaneously.
After loading a Superloop, always plug the Syr port on the Injection valve with a Stop plug.
With a Superloop connected to the valve, an over-pressure may be created during injection.
Note:
Prepare the Superloop
To avoid injecting air into the system flow path, the Superloop should be prefilled with buffer manually,
before fitting the Superloop to the system.
Read the instruction for the Superloop to be used.
Note:
Connect the Superloop
Follow the instruction below to connect the Superloop to the Injection valve or to the Loop valve.
Action Step
Attach the Superloop to the instrument using a Column holder. 1
Connect a piece of tubing from the top of the Superloop to: 2
• port LoopE on the Injection valve
or
• a E port, eg., 1E, on the Loop valve
ÄKTA pure User Manual 29119969 AB 183
5 Operation
5.7 Sample application
5.7.2 Sample application using a Superloop
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