Setting Up the Run List
Reagent Settings
Before you can specify addition of reagent to any samples, first designate one or more positions in the
plate map as reagent positions. Then, for a selected sample position, you can select a reagent
position from the dropdown list and enter the required volume of reagent to add to the sample, in the
range of 2–20 µl. You can instruct the system to mix the reagent after it is added by aspirating and
dispensing the combined sample and reagent several times. You can also choose to add an agitation
step to the position to enhance mixing.
Positions in the plate map on which reagent will be added appear as
Assigning Position Types
Tip:
This procedure explains how to assign position types for a single-panel experiment. If
needed, you can set up a single plate or tube rack with multiple experimental panels. For more
information, see
Multipanel Experiments on page 87
and
Setting Up Multiple Panels on page
Assign positions based on their type: Wash, Sample, Reagent, or Setup (control).
For detailed information on position types and selecting positions, see
and
Selected Media Type on page 72
Note:
Wash positions contain wash solution. Reagent positions contain reagent that can be
added to other positions in the plate map.
User Guide | 197
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Страница 3: ...ZE5 Cell Analyzer and Everest Software User Guide Software Version 2 0 ...
Страница 14: ...Table of Contents xii ZE5 Cell Analyzer and Everest Software ...
Страница 50: ...Chapter 2 Hardware Description 48 ZE5 Cell Analyzer and Everest Software ...
Страница 82: ...Chapter 4 Everest Software 80 ZE5 Cell Analyzer and Everest Software ...
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Страница 92: ...Chapter 4 Everest Software 90 ZE5 Cell Analyzer and Everest Software ...
Страница 104: ...Chapter 4 Everest Software 102 ZE5 Cell Analyzer and Everest Software ...
Страница 109: ...Managing Users 9 Click OK to save the new user account User Guide 107 ...
Страница 120: ...Chapter 5 Configuring the System 118 ZE5 Cell Analyzer and Everest Software ...
Страница 125: ...Working with Optical Filter Configurations User Guide 123 ...
Страница 126: ...Chapter 5 Configuring the System 124 ZE5 Cell Analyzer and Everest Software ...
Страница 173: ...Setting Up the Run List The plate setup displays a layout of the selected media User Guide 171 ...
Страница 177: ...Setting Up the Run List User Guide 175 ...
Страница 191: ...Setting Up the Run List User Guide 189 ...
Страница 276: ...Chapter 7 Creating Experiments and Workspaces 274 ZE5 Cell Analyzer and Everest Software ...
Страница 295: ...Setting Up the Experiment 6 Set up samples User Guide 293 ...
Страница 333: ...Decontaminating the System User Guide 331 ...
Страница 380: ...Appendix B Example 9 Color Immunophenotyping Experiment 378 ZE5 Cell Analyzer and Everest Software ...
Страница 384: ...Appendix C Optional External DI Water and Waste Ports 382 ZE5 Cell Analyzer and Everest Software ...
Страница 392: ...Appendix F References 390 ZE5 Cell Analyzer and Everest Software ...
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