Chapter 6 Daily Routine
Notice the 90 μm filter at the bottom of the fluidics line.
4.
Refill the bottles with DI water or replace them with new bottles.
5.
Carefully place the cap assemblies onto the bottles and tighten the caps.
Important:
n
Ensure that the filter falls to the corner of the bottle diagonally opposite from the cap, so that
the entire contents of the bottle can be drawn into the instrument without risking introduction
of air.
n
Ensure that the bottle cap is firmly tightened; otherwise, leakage might occur.
6.
Place the sheath bottles back into the instrument.
7.
Attach the cap assembly tubing to the connectors on the instrument.
Tip:
An audible click indicates that the tubing is connected.
Replacing Sheath Additive and Cleaner Bottles
The sheath additive and cleaner bottles are located at the bottom of the fluidics chamber. They can be
refilled or replaced at any time other than during shutdown.
Sheath additive and cleaner bottle connections are specifically keyed to their respective ports and
cannot be connected to the wrong ports: the white-capped sheath additive bottle always goes on the
left and the blue-capped cleaner bottle always goes on the right.
Important:
When filling the sheath additive or cleaner bottles:
n
Fill the bottle only to the fill line to ensure that liquid does not leak from the vent in the cap.
n
Ensure that the tubing that extends into the bottle is not contaminated during the refill procedure.
To refill an additive or cleaner bottle
1.
Push the quick connect tab on the connection to disengage the tube from the instrument.
148 | ZE5 Cell Analyzer and Everest Software
Содержание ZE5
Страница 1: ...ZE5 Cell Analyzer and Everest Software User Guide Version 2 0 ...
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Страница 3: ...ZE5 Cell Analyzer and Everest Software User Guide Software Version 2 0 ...
Страница 14: ...Table of Contents xii ZE5 Cell Analyzer and Everest Software ...
Страница 50: ...Chapter 2 Hardware Description 48 ZE5 Cell Analyzer and Everest Software ...
Страница 82: ...Chapter 4 Everest Software 80 ZE5 Cell Analyzer and Everest Software ...
Страница 84: ...Chapter 4 Everest Software 82 ZE5 Cell Analyzer and Everest Software ...
Страница 92: ...Chapter 4 Everest Software 90 ZE5 Cell Analyzer and Everest Software ...
Страница 104: ...Chapter 4 Everest Software 102 ZE5 Cell Analyzer and Everest Software ...
Страница 109: ...Managing Users 9 Click OK to save the new user account User Guide 107 ...
Страница 120: ...Chapter 5 Configuring the System 118 ZE5 Cell Analyzer and Everest Software ...
Страница 125: ...Working with Optical Filter Configurations User Guide 123 ...
Страница 126: ...Chapter 5 Configuring the System 124 ZE5 Cell Analyzer and Everest Software ...
Страница 173: ...Setting Up the Run List The plate setup displays a layout of the selected media User Guide 171 ...
Страница 177: ...Setting Up the Run List User Guide 175 ...
Страница 191: ...Setting Up the Run List User Guide 189 ...
Страница 276: ...Chapter 7 Creating Experiments and Workspaces 274 ZE5 Cell Analyzer and Everest Software ...
Страница 295: ...Setting Up the Experiment 6 Set up samples User Guide 293 ...
Страница 333: ...Decontaminating the System User Guide 331 ...
Страница 380: ...Appendix B Example 9 Color Immunophenotyping Experiment 378 ZE5 Cell Analyzer and Everest Software ...
Страница 384: ...Appendix C Optional External DI Water and Waste Ports 382 ZE5 Cell Analyzer and Everest Software ...
Страница 392: ...Appendix F References 390 ZE5 Cell Analyzer and Everest Software ...
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