Glossary
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C
T
Experiments
224
Sample DNA (10
✕
)
In the 7500 software, a reaction component displayed on the Reaction Mix Calculations tab
of the Reaction Setup screen. The software assumes the sample DNA is added to the
reaction mix at a 10
✕
concentration. For example, if the reaction volume is 20
µ
L, the
calculated volume of sample for 1 reaction is 2
µ
L.
Sample Library
In the 7500 software, a collection of samples. The Sample Library contains the sample
name and the sample color.
Sample or Standard
(10
✕
)
In the 7500 software, a reaction component displayed on the Reaction Mix Calculations tab
of the Reaction Setup screen. The software assumes the sample or standard is added to the
reaction mix at a 10
✕
concentration. For example, if the reaction volume is 20
µ
L, the
calculated volume of sample or standard for 1 reaction is 2
µ
L.
sample/SNP assay
reaction
In genotyping experiments, the combination of which sample to test and which SNP assay
to perform in one PCR reaction. Each PCR reaction can contain only one sample and one
SNP assay.
sample/target
reaction
In quantitation experiments, the combination of which sample to test and which target to
detect and quantify in one PCR reaction. In the Design Wizard, you can detect and quantify
only one target in one PCR reaction. Use Advanced Setup to detect and quantify more than
one target in one PCR reaction.
serial factor
In the 7500 software, a numerical value that defines the sequence of quantities in the
standard curve. The serial factor and the starting quantity are used to calculate the standard
quantity for each point in the standard curve. For example, if the standard curve is defined
with a serial factor of 1:10 or 10
✕
, the difference between any 2 adjacent points in the curve
is 10-fold.
series
See standard dilution series.
slope
Regression coefficient calculated from the regression line in the standard curve. The slope
indicates the PCR amplification efficiency for the assay. A slope of
−
3.32 indicates 100%
amplification efficiency. See also amplification efficiency (EFF%) and regression line.
SNP
Abbreviation for single nucleotide polymorphism. The SNP can consist of a base difference
or an insertion or deletion of one base.
SNP assay
Used in genotyping experiments, a PCR reaction that contains primers to amplify the SNP
and two probes to detect different alleles.
SNP Assay Library
In the 7500 software, a collection of SNP assays to add to genotyping experiments. The SNP
assays in the library contain the SNP assay name, SNP assay color, and for each allele, the
allele name or base(s), reporter, quencher, and allele colors. The SNP assays in the library
may also contain the assay ID and comments about the SNP assay.
region of interest
(ROI) calibration
Type of 7500/7500 Fast system calibration in which the system maps the positions of the
wells in the sample block. ROI calibration data are used so that the software can associate
increases in fluorescence during a run with specific wells in the reaction plate.
stage
In the thermal profile, a group of one or more steps. There are three types of stages: holding
stage (including pre-PCR read and post-PCR read), cycling stage (also called amplification
stage), and melt curve stage.
