OPERATION
Axioskop 2 FS
plus
Carl Zeiss
Illumination and contrasting techniques
Axioskop 2 FS
MOT
3-26
B 40-076 e 02/01
(3)
Transmitted-light phase contrast settings on manual Axioskop
2
FS
plus and
Axioskop 2 FS
MOT
•
Swing phase contrast objective, e.g. Ph 1, in
the beam path.
•
Insert phase stop labelled like the phase
contrast objective, e.g. Ph 1, using the turret
disk of the universal condenser.
•
To check centering and congruence of the
bright phase stop (in condenser) with the dark
phase ring (in objective), remove one eyepiece
from the tube and replace it with the centering
telescope. Use the correction device of the
centering telescope to focus on the phase stop
and the phase ring in the objective exit pupil.
•
If congruence is not perfect (3-20/
A
), the two
SW 1.5 screwdrivers (3-19/
1
and
4
) must be
used on the two centering screws (3-19/
2
and
3
) to recenter the bright phase stop until
complete congruence with the dark phase ring
is achieved (3-20/
B
).
•
Insert eyepiece in the tube again.
Normally, however, centering is not required, since
the phase stops are factory-centered and the
centering is retained even if the universal
condenser is removed from the condenser carrier
and attached again.
To enhance the image contrast, an interference
wide-band filter, green 32 x 4, can be placed on
the luminous-field diaphragm or inserted in the
filter mount (if available).
Complete phase contrast is only achieved if the
bright phase stop (in condenser) and the dark
phase ring in objective) are exactly congruent in
the illumination beam path (3-20/
B
).
Fig. 3-19
Centering of phase stop on
universal condenser
Fig. 3-20
Centering of phase stop (bright in
condenser) to phase ring (dark in
objective)
Summary of Contents for Axioskop 2 FS mot
Page 1: ...Operating Manual Axioskop 2 FS plus FS MOT Research Microscope...
Page 14: ...INTRODUCTION Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 0 14 B 40 076 e 02 01...
Page 110: ...OPERATION Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 3 40 B 40 076 e 02 01...
Page 122: ...ANNEX Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT A 2 B 40 076 e 02 01...