ZEISS
FIRST-TIME SET-UP
Axio Imager 2
Changing the filter set in the reflector module FL P&C
42
430000-7544-001
01/2016
3.12
Changing the filter set in the reflector module FL P&C
The filter sets for the FL P&C reflector module can be combined and assembled individually by the
customer.
Insert only fluorescence filters with a free aperture of
≥
22 mm, otherwise the image may be masked.
Make sure this requirement is met when using filters from other manufacturers.
Filter sets or fully assembled FL P&C reflector modules can be ordered from ZEISS.
•
Remove reflector module FL P&C (Fig. 20/
3
)
from the reflector turret and place it on a
surface (refer to Section 3.11).
•
Use mounting plate (Fig. 20/
6
) from the tool kit
to unscrew retaining ring (Fig. 20/
1
).
•
Turn the reflector module and let the filter
(Fig. 20/
2
or
5
) drop out onto a soft surface.
•
Insert the barrier filter (emission filter) at
(Fig. 20/
2
), the exciter filter at (Fig. 20/
5
). Secure
both filters by means of retaining rings
(Fig. 20/
1
).
The barrier filter and exciter filter may have a designation and an arrow on their circumference. The
arrow indicates the direction in which the particular filter is to be installed in the reflector module; it must
always point inwards (refer to arrows in Fig. 20).
To minimize image offset during multiple fluorescence image captures, an additional label can be
provided on the barrier filter to indicate the position of the wedge angle.
This label should be aligned to the orientation groove (Fig. 20/
4
) when you insert the barrier filter in the
reflector module. This is to ensure that the wedge angle of the barrier filters is in the same defined
position in the reflector modules, thus compensating or minimizing the already minimal module-to-
module image shift when ZEISS filter sets are used.
Fig. 20
Changing the filter set in reflector
module FL P&C
