ZEISS
OPERATION
Axio Imager 2
Relocate an event on a sample carrier
156
430000-7544-001
01/2016
4.10
Relocate an event on a sample carrier
4.10.1
Microscope equipment and basic settings
The basic setting on the microscope corresponds to the preparatory steps for capturing large
overview shots, see Section 4.9 on page 154.
4.10.2
Calibration of the stage
After basic adjustment of the microscope, the stage needs to be calibrated.
Proceed as follows:
1.
Calibrate the stage using the software. Define the zero position of the stage and the polarity of the
axes.
2.
If necessary select a suitable specimen holder (carrier, slide, etc.) in the software.
3.
Set a reference point on the sample carrier (slide).
This is normally set as the zero point of the specimen using the software (or on the stand) to give the
specimen unique reference coordinates. This allows each marked event to be retrieved at any time
regardless of the stand or the stage used.
The reference point should be selected for the particular sample carrier so that it can be
retrieved on all relevant systems.
After setting the reference point you can use your imaging software to restore previously stored
coordinates on your sample.
