9.
Select an action depending on whether the custom dye calibration passed or
failed.
Calibration
status
Action
Passed
1. Tap
Accept Results
or
Reject Results
.
Note:
Accepting the results saves the calibration data to the
instrument and overwrites existing data.
2. (
Optional
) Tap
Transfer EDS
to transfer the calibration data to
a USB.
Failed
1. Perform the calibration again using a new custom dye plate.
Create the plate using the next dye concentration greater than
the optimal dye concentration (see “Determine the optimal dye
concentration” on page 100).
2. For more information, see “Troubleshoot calibration failure” on
10.
Unload the plate from the instrument.
Note:
You must also add the custom dye to the desktop software dye library before
you create, run, or analyse experiments that use custom dyes.
Note:
To perform a custom melt experiment, you can either create a Standard Curve
or a Custom experiment with melt, then specify the data points per degree in the
method.
Chapter 7
Calibrate and verify instrument performance
Calibrate for a custom melt curve run
7
106
QuantStudio
™
6 Pro and 7 Pro Real-Time PCR Systems User Guide
