Calibrate and verify instrument
performance
■
Instrument and block calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
■
Calibration and verification schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
■
Calibration descriptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
■
View the calibration status and set reminders . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
■
Export a calibration report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
■
Perform ROI/uniformity, background, and dye calibrations . . . . . . . . . . . . . . . . 84
■
Perform verification using RNase P plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
■
Calibrate custom dyes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
■
Calibrate for a custom melt curve run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Instrument and block calibration
The QuantStudio
™
6 Pro System and the QuantStudio
™
7 Pro System have
interchangeable blocks. The calibration status applies to the combination of the
instrument and the block.
If a block is moved to an instrument that it has not been calibrated on, that
combination of block and instrument needs to be calibrated. This applies even if the
block has previously been calibrated on a different instrument.
If a block is moved back to an instrument that is has previously been calibrated on, it
does not need to be calibrated again. It needs to be calibrated again if the calibration
is expired.
A run can still be started if a calibration of the block on the instrument is expired. A
run cannot be started if a block has never been calibrated on the instrument.
7
QuantStudio
™
6 Pro and 7 Pro Real-Time PCR Systems User Guide
79
