Thermo Scientific Cryofuge 5500i GMP Instructions For Use Manual Download Page 48

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Operation

Check carefully whether your tubes

are approved for the respective

RCF value. If not, reduce speed.

Filling the centrifuge tubes

For common borosilicate glass tubes, the maximum

permissible RCF is limited to 4000 x g!
The tube manufacturers normally limit the respective

maximum permissible RCF value to the fixed angle

rotor.

Maximum permissible load difference

The smaller the imbalance of the centri-

fuge

the better the separation effect, be-

cause there are no turbulences in the

separated zones caused by vibrations.

Therefore it is important, that the tubes are balanced

properly.
The permissible load difference depends on many

factors (e.g. rotor, loading). For the swinging bucket

rotors LH-4000 and LH-4000W this difference

amounts to at least 30 g in opposite carriers.

Please note that for the same RCF value

the stress for the tubes in a swinging-

bucket rotor is higher!

Because of the higher difference of the

radii (r

max

- r

min

) the pressure of the liquid column to

the tube bottom is considerably higher and strongly

depended on filling.
Plastic tubes – especially for the highest load (speed,

temperature) – have a limited service life only and

must be replaced. For detailed questions please

contact the manufacturer of the plastic tubes.

Summary of Contents for Cryofuge 5500i GMP

Page 1: ...Thermo Scientific Cryofuge 5500i GMP Instructions for Use 20057801 e 07 2020 Visit us online to register your warranty thermofisher com labwarranty ...

Page 2: ...not provide aid against improper handling and thus against dam age to individuals and surrounding The manual contains chapters on Safety regulations Instrument description Transport and installation of the centrifuge Rotors and accessories Use of the centrifuge Maintenance and care Troubleshooting Technical data Index Overleaf you will find a graphical illustration of the control panel and a surve...

Page 3: ...ration profiles temperature program display key switch program selection program storage key speed RCF run time switch key rpm RCF display quick run start open lid stop bucket selection set keys pre temperature regulation The mains switch is located on the right hand sidepanel Pressed up ON I Pressed down OFF 0 set keys ...

Page 4: ...number Pretemp pre temp function set keys stepwise increase decrease of set values Short pressing of any of these keys switching from actual to set value signaled by flashing display before start lid closed 0 with flashing point rotor not yetidentified error code will flash in display Run time time selection remaining run time up to 0 continuous run time passed operation hold in minutes and second...

Page 5: ...sories 22 Handling rotors 29 Swinging Bucket Rotors 29 LH 4000 29 LH 4000 W 29 Contents BIOshield Rotor 30 Highplate Rotor 31 Handling micro plates 32 Diagnostik Rotor 33 Highconic Rotor 33 LAC 250 34 Aerosol tight operation 36 Checking of aerosol tight bio containment 40 Operation 41 Switching on the centrifuge 41 Actuating the lid 41 Opening the lid 41 Closing the lid 41 Installing the rotor 42 ...

Page 6: ... Program display 57 Entering changing a program 57 Centrifuging with a program 58 Quick Run 58 Removing the rotor 59 Audible alarm 59 Putting the centrifuge out of operation 60 WEEE Compliance 60 Maintenance and care 61 Maintenance to be performed by the customer 61 Cleaning 61 Disinfection 62 Decontamination 64 Autoclaving 64 The Thermo Fisher Scientific Service offer 65 Warranty conditions 65 Tr...

Page 7: ...t all times Proper use The centrifuge is designed to separate liquid suspended materials having different densities and particle size respectively maximum sample density is 1 2 g cm ml at maximum speed Improper use During a run a safety zone of 30 cm around the centri fuge must be maintained where neither persons nor hazardous materials may be present The centrifuge may cause harm to its user or o...

Page 8: ...al accessories for the centrifuge The only exceptions are common glass or plastic centri fuge tubes if they are approved for the rotor speed and RCF values Never use the centrifuge unless the rotor is properly installed You may use the centrifuge only with a properly loaded rotor You must not overload the rotor Strictly follow the rules and regulations for cleaning and disinfection If the rotor or...

Page 9: ...lease get acquainted with the details of the test stan dards from the technical data Safety instructions in this manual This symbol denotes potential hazards to persons This symbol denotes potential damage to the centrifuge or parts in its immediate surroundings Attention general danger place General comments are marked with this symbol Before switching on the centrifuge please read this manual ...

Page 10: ...nstructions 6 CAUTION This product is intended for General Laboratory Use It is the customer s responsibility to ensure that the performance of the product is suitable for customer s specific use or application ...

Page 11: ...cal and biotechnological industry In addition the Cryofuge 5500i GMP is a compact centrifuge for blood component separation in transfu sion centers The Cryofuge 5500i GMP is designed especially in accordance with the requirements for GMP Good Manufacturing Practice This is characterized among other things by a completely encapsulated casing which suppresses the emission of particles and thus preve...

Page 12: ...You can open the centrifuge lid only when the cen trifuge is switched on and the rotor has come to a stop Start the centrifuge with properly locked lid only Automatic rotor identification Electronic imbalance detection as a function of rotor SMARTspinTM corrosion protective oil installation set with instrument feet mounting tools and box level Do not tamper with the safetysystem The printed docume...

Page 13: ...cover Control microprocessor controlled by Easycontrol II Operating memory last set parameters remain Program memory values are stored until new data will be entered after starting Functions RCF selection quick run pretemp temperature regulation during standby Acceleration and deceleration profiles 9 acceleration and 9 deceleration profiles Rotor identification automatic SMARTspinTM imbalance dete...

Page 14: ...Speed selection adjustable from 300 rpm to 10 000 rpm in 10 rpm increments RCF selection after actuating RCF switch key the RCF value can be entered Time selection adjustable in seconds up to 9 min 59 sec in the range of 10 min up to 99 min in minutes hold mode continuous operation Run time display in quick run mode in second increments Temperature selection adjustable from 9 C to 40 C in one degr...

Page 15: ...tivates maximum acceleration up to the maximum permissible speed of rotor upon key release centrifuge stops with maximum deceleration power Diagnostic messages alternating display rotor maximum speed or RCF speed selected exceeds max speed of the rotor lid has not been lifted off the lock during opening display lift lid manual lifting of lid required general instrument malfunction error messages w...

Page 16: ...Description of the Cryofuge 12 Notes ...

Page 17: ... kept around the centrifuge No dangerous materials may be located there during the centrifugation The floor must be stable and resonance free The centrifuge must be protected against heat and strong solar radiation Transporting centrifuge UV radiation reduce the durability of plastics Place the centrifuge as well as ro tors and accessories of plastic so that they are not exposed to direct solar ra...

Page 18: ...Before the use 14 Mains switch Scanner connection Optional for HERANET Service inter Mains inlet Water drain Zero potential outlet for a water circulation Water supply ...

Page 19: ...the type designation plate is identical to the available mains voltage 4 The mains connection at a plug socket with protec tive grounding takes place The Cryofuge GMP can be connected to a closed circuit in house water circulation system The connection to a water circulation cooler e g HWK 4600 A Order No 76007473 is also possible Commissioning Connect plug with plug socket Ensure water supply and...

Page 20: ...Before the use 16 Notes ...

Page 21: ...addition there are adapters and reduction sleeves for a variety of commercially available tubes and bottles For a complete list of accessories together with tech nical data and order numbers refer to our current sales documentation For more information you can visit our web site at http www thermofisher com ...

Page 22: ...issible load g 4 x 1400 4 x 1500 Maximum speed nmax rpm 4400 4400 Maximum RCF value at nmax 5346 4654 Radius max min cm 24 7 8 8 21 5 11 5 Acceleration deceleration time s 85 85 85 85 Min temperature at nmax relative to room temperature 23 C 10 10 Speed at 4 C rpm 4100 4100 k factor S h 13487 8244 Aerosol tight 1 with cap 75006421 with cap 75006479 Autoclavable 121 C 121 C 1 Checked by TÜV Technic...

Page 23: ...4 x 1900 Maximum speed nmax rpm 4400 4400 4400 Maximum RCF value at nmax 5346 4654 5476 Radius max min cm 24 7 8 8 21 5 11 5 25 3 11 0 Acceleration deceleration time s 95 85 95 85 110 90 Min temperature at nmax relative to room temperature 23 C 4 4 4 Speed at 4 C rpm 4400 4400 4400 k factor S h 13487 8244 10885 Aerosol tight 1 with cap 75006421 with cap 75006479 No Autoclavable 121 C 121 C No 1 Ch...

Page 24: ... Maximum speed nmax rpm 5850 5650 3500 Maximum RCF value at nmax 6963 5889 2547 Radius max min cm 18 2 13 8 16 5 10 5 18 6 14 1 Acceleration deceleration time s 85 100 80 100 60 65 Min temperature at nmax relative to room temperature 23 C 1 0 0 Speed at 4 C rpm 5850 5650 3500 k factor S h Aerosol tight 1 Yes Yes No Autoclavable 121 C No No 1 Checked by TÜV Technical Supervision Association Product...

Page 25: ... speed nmax rpm 8500 10000 Maximum RCF value at nmax 10016 15317 Radius max min cm 12 4 6 0 13 7 9 5 Angle of incidence 45 23 Acceleration deceleration time s 75 75 110 110 Min temperature at nmax relative to room temperature 23 C 0 1 Speed at 4 C rpm 8500 10000 k factor S h 2545 926 Aerosol tight 1 loading see page 39 Yes No Autoclavable 121 C No 1 Checked by CAMR Porton Down UK ...

Page 26: ...77 31 0 28 orange 75008138 7 x 45 50 ml flat and round bottom 29 5 x 177 31 0 28 blue 75006493 4 x 50 ml DIN 34 5 x 177 39 0 16 green 75008140 5 x 50 ml conical 29 5 x 177 35 5 20 light green 75006533 2 x 100 ml DIN 45 0 x 177 47 5 8 light blue 75008142 1 x 150 ml DIN 180 ml bottle 56 5 x 132 128 4 white 75006498 1 x 175 225 ml conical1 250 ml bottle 62 0 x 177 75 0 4 nature 75008144 1 x 250 ml 59...

Page 27: ...dapter 2 Accessories for blood bag centrifuga tion in double blood bag bucket 75006436 order no Plastic insert M Set of 2 pairs for 450 ml Double blood bags 76007667 Plastic insert L Set of 2 pairs for 450 ml Triple blood bags 76007647 Plastic insert XL Set of 2 pairs for 450 500 ml Quad Triple blood bags 76007657 Plastic insert XXL Set of 2 pairs for 500 ml Quint Quad Triple blood bags with in li...

Page 28: ...153 24 0 72 olive brown 75006456 9 x 14 15 ml with flange 18 3 x 122 147 25 0 72 brown 75006492 6 x 25 ml DIN 25 0 x 127 149 28 0 48 orange 75006457 4 x 25 50 ml universal container 25 5 x 132 145 32 0 32 blue green 75006459 4 x 45 50 ml flat and round bottom 29 5 x 125 145 35 0 32 blue 75006491 3 x 50 ml DIN 34 5 x 127 148 38 0 24 green 75006460 4 x 50 ml conical 29 5 x 129 149 35 5 32 light gree...

Page 29: ...4 x 15 ml conical US urine 16 5 x 120 147 3 23 0 96 nature 75006468 for cyto system 8 black 76006466 for Centri Lab Adapter type A 8 black 76006467 for 1 x Centri Lab Adapter type D 4 75006499 Plate holder for micro test plates Basic area 127 x 85 max height of charge 110 mm 75006486 Aerosol tight caps 2 pieces incl seals and lubricants 75006479 3 The maximum tube length depends on the position in...

Page 30: ...ml conical US urine 16 5 x 120 24 0 36 olive brown 75006456 9 x 14 15 ml with flange 18 3 x 117 25 0 36 brown 75006492 6 x 25 ml DIN 25 0 x 117 28 0 24 orange 75006457 4 x 25 50 ml universal container 25 5 x 117 32 0 16 blue green 75006459 4 x 45 50 ml flat and round bottom 29 5 x 117 35 0 16 blue 75006491 3 x 50 ml DIN 34 5 x 117 38 0 12 green 75006460 4 x 50 ml conical 29 5 x 118 35 5 16 light g...

Page 31: ...22 Beckmann 120 7 2 x 2 75006423 Table 2 Adapter 6 Adapter for Highconic Rotor 75003057 max tube dimensions d x length mm number per adapter number per rotor color order no 1 5 ml micro tubes 11 x 58 4 24 nature 76002905 3 5 ml 11 x 103 4 24 nature 75003091 6 5 ml 13 x 115 2 12 nature 75003092 12 ml 16 x 96 2 12 nature 75003093 16 ml 18 x 124 1 6 nature 76002906 38 ml 25 x 112 1 6 nature 75003094 ...

Page 32: ...2002 1 x 150 125 ml Corex bottle 53 x 132 6 yellow 00372 1 x 150 ml thick walled tubes 45 x 132 6 white 00458 1 x 100 ml Pyrex bottle 44 x 137 6 yellow 00371 12 x 5 4 ml 12 x 75 72 red 00388 7 x 12 ml 16 x 100 42 purple 00389 3 x 30 ml Corex bottle 24 x 105 18 white 00449 5 x 14 ml Pyrex bottle 18 x 120 30 grey 00456 1 x 50 35 ml conical 30 x 115 6 white 03072 5 x 3 ml Pyrex tube 10 x 75 30 green ...

Page 33: ...mbalance On swinging bucket rotors at regular intervals apply a light coating of lubricant to the rotor body trunnion pins and to the cor responding mating surfaces of the buckets Lubricant 70006692 is supplied with the centrifuge LH 4000 LH 4000 W Diagnostik Rotor These rotors have a slide coating which guarantees perfect operation without additional lubrication of body trunnion pins for many yea...

Page 34: ...d turning the lid locking knob at the same time Store the BIOshield Rotor with the lid removed after cleaning This will enable the rotor to dry completely Exceeding the maximum number of cycles can result in rotor fracture leading to a destruction of the cen trifuge max cycles 22000 31000 max speed 5850 rpm 5350 rpm The rotor has to be replaced upon reaching the end of its service life depending o...

Page 35: ...he rotor lid open frequent use 20 runs day 220 days year average use 7 runs day 220 days year 5 years 14 years 7 years 20 years Always maintain the rotor in the recom mended manner The rotor and accessories must be cleaned and inspected regularly do not use when showing signs of corrosion or cracking Store the Highplate Rotor with the lid removed after cleaning This will enable the rotor to dry th...

Page 36: ...the rubber bottom is placed in the cut outs of the plate holder Deep well plates can also be inserted directly into the swing without plate holder In case of problems please ask the manufacturer for higher rated plates e g plates from PP Make sure the rotor charge isbalanced The plate carriers are inserted as shown in the illus tration below Highplate Rotor Plate holders for micro test plates 7500...

Page 37: ... racks could tip over and tubes could be damaged Highconic Rotor In case longer tubes shall be centrifuged precluding the complete locking of the rotor lid it is allowed to operate the rotor without lid up to a maximum speed of 4000 rpm Above 4000 rpm the rotor may not be operated without lid in any case Otherwise the rotor could be de stroyed ...

Page 38: ...show changed characteristics with regard to chemical resistance Organic solutions strong acids or bases can initiate premature material weakness Before use carefully and regularly inspect rotors made of carbon fiber composite material for surface wear The rotor may not be operated if due to damage fibers escape the rotor surface Wear suitable protective gloves e g of leather when handling damaged ...

Page 39: ...apped inside a 5 minute run at 300 rpm will remove it Procedure in case of damage In case of rotor failure wrong handling can cause dangers by the carbon fibers of the rotor In case of damage allow 30 minutes for particles to settle before accessing the rotor chamber Particles have to be removed from the surface with humid cloth or sponges Do not sweep or vacuum dry Dispose rotor pieces and partic...

Page 40: ... only use the special lubricant 76003500 Spare parts are delivered with the rotor or may be ordered separately Correct operation when filling the sample tubes and closing the rotor lid are prerequisites for aerosol bio containment Replace damaged or clouded caps and lids of rotors and tubes imme diately When centrifuging dangerous sam ples aerosol tight rotors and tubes may only be opened in an ap...

Page 41: ... turn the lid clockwise by 1 grip areas approx 15 Use the setting marks on the bucket as an orientation If the windshield rotors are filled with samples outside the centrifuge e g in a safety work bench it is es sential that during the insertion into the centrifuge the rotor is kept in a vertical position Grip areas Bucket mark The aerosol tight bio containment of windshield rotors is only secured...

Page 42: ... before closing Please flap both lock levers upwards Now you can eas ily put the cap on the bucket By flapping down the lever the bucket is being locked Both of the levers must click in order to achieve aerosol tight bio containment Levers not being flapped down cause damages of the caps dur ing centrifugation ...

Page 43: ...aerosol tightly The hexagon screwdriver of the pliers should be used as a support tool to fasten and loosen the lid of the fixed angle rotor in order to achieve secure closing insert the hexagon through the hole in the screw cap conical cell culture tube in Highconic Rotor 75003057 Nominal volume permissible filling volume 15 ml 14 ml 50 ml 49 ml ...

Page 44: ...ation of gas mix Finally buckets respectively rotor lid and lid seal have to be dried It is very important that all the seals and seal surfaces are being tested for wear and damages like cracks scratches and embrittlement carefully As a quick test there is the possibility to check the aerosol tight buckets and fixed angle rotors according to the following procedure grease slightly all seals Fill t...

Page 45: ...speed should both read 0 The display of the accelera tion deceleration curve depends on the last set value The following figure gives an example of possible readings A detailed description of possible settings is given below in this chapter Actuating the lid Opening the lid Press the open lid key If the message Lift lid appears you must lift the lid slightly Emergency release in case of malfunctio...

Page 46: ...foreign ma terial or sample residues out of chamber before use 2 Check whether the collet chuck is loose collet chuck moves freely on the spindle If not loosen the rotor seat using the socket wrench supplied 3 Place the rotor on the drive shaft so that the rotor chuck is located precisely above the center 4 The rotor must glide freely down the collet chuck until it hits the lower stop 5 If you hav...

Page 47: ... enormous centrifugal forces The rotors are designed in a way having sufficient residual strength even at the highest permissible speed n perm n max However this safety system presupposes that the maximum permissible load of the rotor is not ex ceeded Please note the data on the maximum permissible load and maximum speed in chapter Rotors and accessories on page 17 nperm permissible speed nmax max...

Page 48: ...rations Therefore it is important that the tubes are balanced properly The permissible load difference depends on many factors e g rotor loading For the swinging bucket rotors LH 4000 and LH 4000W this difference amounts to at least 30 g in opposite carriers Please note that for the same RCF value the stress for the tubes in a swinging bucket rotor is higher Because of the higher difference of the...

Page 49: ...e wear of the drive Inserting the centrifuge tubes The rotor has to be loaded symmetrically When loading the rotor only partially you have to ensure that opposite bore holes always receive tubes of equal weight when centrifuging a single sample place a centrifuge tube e g filled with water After placing the tubes close the rotor lid Fixed angle rotors Proper loading Improper loading ...

Page 50: ...oper loading Improper loading For swinging bucket rotors please note the symmetrical loading of the buckets These examples are to be applied to the other rotors in an analogous manner Mixed loading with different bucket types is not allowed ...

Page 51: ...cket only one sample shall be centrifuged attention has to be paid to merely loading the external chambers An exclusive loading of the internal chambers could result in an overswing of the bucket For optimum operation with partial loading please use the corresponding amount of tare weights 75007668 for balancing see Adapters and Accessories from page 22 ...

Page 52: ...gainst alternations The user can use but not alternate all programs The centrifugation program in the mainmemory can be alternated Quick Run and pre temperature regulationcan be used Position C Program and main memory are secured against alternations All set parameters are blocked yet the current cen trifugation program can be driven as often as needed with the control keys start stop open lid The...

Page 53: ... speed mode selection key you decide whether you want to entry and display respectively speed and RCF Bucket selection for swinging bucket rotors The automatic rotor identification will recognize the rotor cross of the swinging bucket rotor If various buckets or carriers can be in stalled in the rotor cross the correspond ing type of bucket must be selected The bucket selection affects the correct...

Page 54: ...by one step 10 rpm at a time 3 If you hold down the key the display changes con tinuously and slowly at first and after a few sec onds at an accelerated pace up respectivelydown 4 Release the key as soon as you have reached the desired value and adjust if necessary by repeat edly pressing the key The decimal place flashes for a few seconds then changes to actual value display The speed is now stor...

Page 55: ...ed is lower than 300 rpm RCF value explanation The relative centrifugal force RCF is given in multi ples of the earth gravity g It is a unit free number that allows the comparison of the efficiency of separation or sedimentation of diverse instruments since it is independent of the instrument used The only values entered in the equation are radius and speed of cen trifugation 2 RCF 11 18 r r radiu...

Page 56: ...n now raise or lower the run time step by step 3 If you keep the selected key pressed the display changes continuously and slowly and after a few seconds at an accelerated pace up respectively down 4 Release the key as soon as you have reached the desired value and adjust if necessary by repeat edly pressing the key The time display flashes for a few seconds then changes to permanent dis play The ...

Page 57: ...election mode is ac tive Pressing the upward key within the speed display you can switch back and forth between the signal menu beep and the time menu t set After selecting the time menu t set you can switch between the standard time mode 00 0 and the lim ited time mode 0 00 by pressing the upward key t K S20 W t time of sedimentation in hours k clearing factor of the rotor k factor s20 W sediment...

Page 58: ...y pressed the display changes continuously and slowly at first and then in accelerated paces up or down 4 Release the key as soon as you have reached the desired value and adjust if necessary by repeat edly pressing the key The temperature display flashes for a few seconds then changes to the actual value mode The tempera ture set value is stored now Pretemp function The Pretemp function permits e...

Page 59: ...may adopt this value by again actuating the start key repeatedly the centrifugation is then continued Otherwise the centrifuge stops and you must enter a permissible value You cannot open the lid during the run Imbalance display If an imbalanced load exists this will be displayed above a speed of approximately 300 rpm through the hint bAL in the speed display The run is terminated and you may rest...

Page 60: ... is displayed At continuous operation If you have chosen continuous operation you must stop the centrifuge manually Press the stop key in the control panel The centrifuge will be deceler ated with the preset deceleration profile The display reads end and you can open the lid by pressing the open lid key and remove the centri fuged material Temperature control during standby The exact regulation wi...

Page 61: ...hange to after identification of the rotor Entering changing a program From the manufacturer all the program places 1 to 9 have been preset with the same values For a change please follow this procedure Select memory place number with the program selection key memory place number flashes Open the program memory by actuating the pro gram memory key possible only if the memory place number flashes a...

Page 62: ...ification of the rotor Quick Run For short term operation the Cryofuge 5500i GMP is equipped with a Quick Run function Short term centrifugation is started by pressing the quick run key continuously it stops as soon as the key is released In this mode the centrifuge accelerates and deceler ates with full power The set speed or RCF is ignored in this case During acceleration the time is counted for...

Page 63: ...r e g in a safety work bench and decontaminate it Audible alarm Accompanying all error messages a warning signal is given out which only is silenced upon actuating any key As an option you can also have the end of a run sig naled acoustically For activating or deactivating this option please press the key in the actual value mode After about 1 sec you get into the selection mode The speed display ...

Page 64: ... the centrifuge is disconnected from the mains Without motor deceleration it takes much more time until the rotor comes to a standstill The centrifuge lid can only be opened automatically if the centrifuge is switched on WEEE Compliance This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2012 19 EU It is marked with the following symbol...

Page 65: ...of hygiene and to prevent corrosion due to contamination sticking to the instru ment and its accessories Clean them with mild agents of pH values ranging from 6 to 8 Immediately after cleaning dry the aluminum parts or put them into a warm air dryer at a temperature not exceeding 50 C Unsuitable cleaning agents or disin fection procedures may damage the centrifuge and its accessories If you intend...

Page 66: ...ith the drive shaft and the ball bearing Organic solvents may decompose the lubricant of the motor bearing The drive shaft may block Infectious material could enter the cen trifuge if spills or tube breakage occur Danger of infection may occur upon contact Take appropriate protective measures for personnel Mind the permissible filling volumes and loading limits for the tubes In case of contaminati...

Page 67: ...chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft 4 Remove the centrifuge tubes and adapters and disinfect them or dispose of them as necessary 5 Treat the rotor and the rotor lid according to the instructions given for the disinfectant soaking in liquid or spraying You must strictly observe the specified action times 6 Turn the rotor head down and drain off t...

Page 68: ...oft absorbent cloth Dis pose of all washing solutions in appropriate radioactive waste containers Autoclaving Check whether autoclaving is permitted see label on the rotor body and the rotor lid For the autoclavable parts the permissible autoclaving cycle is 20 min at 121 C Remove the rotor lid together with the centrifuge tubes and the adapter The rotor has to be cleaned and rinsed with distilled...

Page 69: ...racts covering it Inspection costs are charged as flatrate contracts Necessary repairs are carried out free of cost within the warranty conditions and against payment after expiration of the warranty period Warrantyconditions The warranty period starts with the day of delivery Within the warranty period the centrifuge is repaired or replaced free of cost if there are provable faults in materials o...

Page 70: ...Maintenance and care 66 ...

Page 71: ...l mains plug 3 On the right and left hand side of the front screen there is a plastic plug which you can remove with a screwdriver or a knife Emergency release Rotor can run at high speed Touch ing it may cause severe injuries Always wait several minutes until the rotor has come to a complete standstill without deceleration Without power supply the brake is out of operation and deceleration takes ...

Page 72: ...l open and you can remove your samples 4 Afterwards push the cord back into the instrument and close the opening by the plug again Once the power failure has been eliminated you can connect the instrument to the mains and switch it on Following the self test of the centrifuge the lid may be closed and locked with the motor ...

Page 73: ...contact customer service Displays fail briefly The drive stops suddenly The rotor stops with de celeration The display shows E 14 Brief interruption of mains voltage 1 Turn off mains switch 2 Check whether the mains plug is connected properly 3 Restart the centrifuge Lid cannot be opened Pressing the open lid key has no effect Lid not correctly engaged or lid warped 1 Check whether mains voltage i...

Page 74: ...properly mounted and loaded 4 Check whether a broken tube damage to the rotor or motor is responsible for the noise If you cannot locate and solve the problem yourself contact cus tomer service Message bAL appears in dis play Rotor stops with decel eration Imbalance switch actuated 1 Open the instrument by pressing open lid key 2 Check whether the rotor is properly loaded 3 Check whether a broken ...

Page 75: ...ing the start key The centrifugation is then continued B Following onset of deceleration you must wait until the rotor has stopped By opening and closing the lid you reset the mes sage rotor After entering a permissible speed you can start again Display OPEN appears al though lid is closed Will not start Lid not properly closed Open the lid and repeat locking procedure Message Lid appears in the d...

Page 76: ...r the malfunction contact customer service E 02 Rotor stops without de celeration to standstill Instrument cannot be operated Internal program error in the memory Switch the instrument off and on again If the error persists con tact customer service E 03 Rotor stops without de celeration to standstill Instrument cannot be operated Error in speed measurement Switch the instrument off and on again I...

Page 77: ...ature 70 C Cooling machine possibly defective E 08 Rotor stops without de celeration to standstill Instrument cannot be operated Over voltage at frequency converter Mains voltage outside the tolerance Deceleration resistance de fective If necessary request customer service E 10 Self test after switching on the centrifuge NV RAM error in program memory Switch the instrument off and on again If the ...

Page 78: ... per mitted for the operation in the respective rotor Please note the hints of chapter Bucket selection of swinging bucket rotors page 49 and compare the permitted rotors and rotor buckets for Cryofuge 5500i GMP in table 1 on page 18 D Following a brief power failure the rotor could not beidentified Switch the instrument off and on again using the mains switch E 15 Rotor stops without de celeratio...

Page 79: ...ot fit the processor E 23 Rotor stops without de Mains voltage too low Excessive pressure in the refrigeration unit or motor over heated 1 Turn instrument off and pull mains plug 2 Check and clean ventilation slots if necessary 3 After about 60 min you can restart the instrument If the error persists contact customer service celeration to standstill or does not start E 24 Self test after switching...

Page 80: ...sing the lid open key 3 Check whether the rotor has been inserted and fastened cor rectly If the error persists contact customer service E 27 Rotor stops without de celeration to standstill Instrument cannot be operated Mains voltage too low Electronics overheated 1 Turn instrument off and pull mains plug 2 Check and clean ventilation slots if necessary 3 After about 60 min you can restart the ins...

Page 81: ...on You can determine the software version as follows 1 Switch the instrument off 2 Switch the instrument on For about 1 sec all displays read Subsequently the following readings will be dis played for 2 seconds each Software version key panel Software version NV RAM version 1 NV RAM version 2 The values in the time display show the actual ver sion The last information displayed is the current cycl...

Page 82: ...ive humidity at 40 C 104 F Ambient temperature permitted 2 C to 40 C run time 1sec 99 min hold continuous operation maximum speed nmax 10000 rpm rotor dependent adjustable in steps of 10 minimum speed nmin 300 rpm maximum RCF at nmax 15317 fixed angle rotor 75006483 maximum kinetic energy 83 3 kNm noise at maximum speed 58 dB A Temperature set range 9 C to 40 C Dimension H x W x D 960 mm x 741 mm ...

Page 83: ...ope 2006 42 EC Machinery Protective goals of 2014 35 EU Low Voltage 2014 30 EU Electromagnetic Compatibility EMC 2011 65 EU RoHS Directive on the Restriction of the use of certain Hazardous Substances in electrical and electronic equipment Region Standard EN 61010 1 EN 61010 2 020 EN 61326 1 Refrigerants Article No Centrifuge Refrigerant Quantity Pressure GWP CO2e 75004478 Thermo Scientific Cryofu...

Page 84: ...Appendix 80 Appendix Acceleration and deceleration profiles On the following pages you will find acceleration and deceleration profiles for each rotor type respectively ...

Page 85: ...ndix 81 9 1 Acceleration profiles LH 4000 75006475 with round bucket 75006477 and double rectangular bucket 75006478 5000 4500 4000 3500 3000 2500 2000 1500 1000 500 0 0 1 2 3 4 5 6 7 8 9 time min speed rpm ...

Page 86: ...pendix 82 Deceleration profiles LH 4000 75006475 with round bucket 75006477 and double rectangular bucket 75006478 5000 4500 4000 3500 3000 2500 2000 1500 1000 500 0 time min 0 9 5 10 15 20 1 25 speed rpm ...

Page 87: ...ix 83 9 1 Acceleration profiles LH 4000W 75006476 with round bucket 75006477 and double rectangular bucket 75006478 5000 4500 4000 3500 3000 2500 2000 1500 1000 500 0 0 1 2 3 4 5 6 7 8 9 10 time min speed rpm ...

Page 88: ...endix time min 84 Deceleration profiles LH 4000W 75006476 with round bucket 75006477 and double rectangular bucket 75006478 5000 4500 4000 3500 3000 2500 2000 1500 1000 500 0 0 5 10 9 15 20 25 1 speed rpm ...

Page 89: ...Appendix 85 9 1 Acceleration profiles LH 4000W 75006476 with double blood bag bucket 75006436 5000 4500 4000 3500 3000 2500 2000 1500 1000 500 0 0 1 2 3 4 5 6 7 8 9 10 time min speed rpm ...

Page 90: ...Appendix time min 86 Deceleration profiles LH 4000W 75006476 with double blood bag bucket 75006436 5000 4500 4000 3500 3000 2500 2000 1500 1000 500 0 0 5 10 9 15 20 25 1 speed rpm ...

Page 91: ...Appendix 87 Acceleration profiles BIOshield Rotor 75006435 Highplate Rotor 75006444 9 1 6000 5000 4000 3000 2000 1000 0 0 1 2 3 4 5 6 7 8 9 10 time min speed rpm ...

Page 92: ...Appendix time min 88 Deceleration profiles BIOshield Rotor 75006435 Highplate Rotor 75006444 6000 5000 4000 3000 2000 1000 0 0 5 10 9 15 20 25 1 speed rpm ...

Page 93: ...Appendix 89 9 1 Acceleration profiles Diagnostik Rotor 75006480 4000 3500 3000 2500 2000 1500 1000 500 0 0 1 2 3 4 5 6 7 8 9 time min speed rpm ...

Page 94: ...Appendix time min 90 9 1 Deceleration profiles Diagnostik Rotor 75006480 3500 3000 2500 2000 1500 1000 500 0 0 5 10 15 20 25 speed rpm ...

Page 95: ...Appendix 91 9 1 Acceleration profiles Highconic Rotor 75003057 9000 8000 7000 6000 5000 4000 3000 2000 1000 0 0 1 2 3 4 5 6 7 8 9 10 time min speed rpm ...

Page 96: ...Appendix 92 Deceleration profiles Highconic Rotor 75003057 9000 8000 7000 6000 5000 4000 3000 2000 1000 0 0 9 5 10 15 20 time min 1 25 speed rpm ...

Page 97: ...Appendix 93 9 1 Acceleration profiles LAC 250 75006483 12000 10000 8000 6000 4000 2000 0 0 1 2 3 4 5 6 7 8 9 10 time min speed rpm ...

Page 98: ...Appendix 94 9 1 Deceleration profiles LAC 250 75006483 10000 9000 8000 7000 6000 5000 4000 3000 2000 1000 0 0 5 10 15 20 25 time min speed rpm ...

Page 99: ...Appendix 95 ...

Page 100: ...ectangular bucket nmax 4400 rpm rmax 21 5 cm rmin 11 5 cm RCF rmax nmax 4654 75006477 Round bucket nmax 4400 rpm rmax 24 7 cm rmin 8 8 cm RCF rmax nmax 5346 Speed RCF diagrams 10000 Speed RCF diagram LH 4000 75006475 LH 4000W 75006476 1000 100 RCF x g ...

Page 101: ...Appendix 97 Speed RCF diagram LH 4000W 75006476 10000 1000 100 10 100 1000 speed rpm 10000 75006436 Double blood bag bucket nmax 4400 rpm rmax 25 3 cm rmin 11 0 cm RCF rmax nmax 5464 RCF x g ...

Page 102: ...Appendix 10 100 1000 10000 speed rpm 98 Speed RCF diagram BIOshield Rotor 75006435 10000 1000 100 nmax 5850 rpm rmax 18 2 cm rmin 13 8 cm RCF rmax nmax 6963 RCF x g ...

Page 103: ...Appendix 99 Speed RCF diagram Highplate Rotor 75006444 10000 1000 100 10 100 1000 10000 speed rpm nmax 5650 rpm rmax 16 5 cm rmin 10 5 cm RCF rmax nmax 5889 RCF x g ...

Page 104: ...Appendix speed rpm 100 nmax 3500 rpm rmax 18 6 cm rmin 14 1 cm RCF rmax nmax 2547 Speed RCF diagram Diagnostik Rotor 75006480 10000 1000 100 10 100 1000 10000 RCF x g ...

Page 105: ...Appendix 101 Speed RCF diagram Highconic Rotor 75003057 100000 10000 1000 100 10 100 1000 10000 speed rpm nmax 8500 rpm rmax 12 4 cm rmin 6 0 cm RCF rmax nmax 10016 RCF x g ...

Page 106: ...Appendix 102 Speed RCF diagram LAC 250 75006483 100000 10000 1000 100 10 100 1000 speed rpm 10000 nmax 10000 rpm rmax 13 7 cm rmin 9 5 cm RCF rmax nmax 15317 RCF x g ...

Page 107: ...Appendix 103 ...

Page 108: ...ible signal switching on off 59 autoclaving 64 autoclaving cycle 64 B basic unit function 9 C centrifuging with a program 58 changing settings during run 55 cleaning 61 closing the lid 41 collet chuck 42 common glass or plastic centrifuge tubes 4 conditions of warranty 65 Conformity 5 connecting 13 contamination necessary measures 62 corrosion 4 corrosive substances protective tubes for corrosive ...

Page 109: ...s 79 emergency lid release 67 entering parameters 48 entering changing a program 57 error codes Lid 71 OPEN with lid closed 71 rotor 71 error codes bAl 70 G general view 8 GMP 7 H handling the centrifuge 4 hazardous substances 3 hazards symbols 5 hints symbol 5 I icons for denoting dangers and potential damage 5 imbalance 44 imbalance 55 imbalance detection 8 infectious material precautions in cas...

Page 110: ... max tube dimensions 22 24 25 26 maximum loading 43 maximum permissible filling volume 39 maximum permissible RCF 55 maximum permissible speed 55 maximum RCF value 18 19 20 21 maximum sample density 3 mechanical lid unlocking cord 68 memory main 48 program 48 micro plates 32 O opening the lid 41 operation short term 58 organic solvents not allowed for cleaning 62 P pathogenic microorganism s prote...

Page 111: ...lay and rotor identification 51 RCF selection 10 RCF setpoint 51 RCF value 51 relative centrifugal force 51 rotor cover 42 rotor identification 8 rotor identification and RCF display 51 rotor identification 51 rotors approved 42 S safety instructions 3 Safety instructions 5 safety measures 3 safety standards 5 safety zone 3 sample density maximum 3 sample tubes are approved 44 security area 13 sel...

Page 112: ... 56 switching from speed to RCF display and vice versa 49 switching on the centrifuge 41 symbols for hazards and dangers 5 program display 57 T technical data 78 temperature control during standby 56 test aerosol tightness 40 time counted forward in quick run mode 58 time of sedimentation 53 tools for installing the rotor 42 toxins protection against 4 transporting 13 tube breakage with infectious...

Page 113: ......

Page 114: ...se consult your local sales represen tative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Australia 61 39757 4300 Austria 43 1 801 40 0 Belgium 32 9 272 54 82 China 800 810 5118 400 650 5118 France 33 2 2803 2180 Germany national toll fr...

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