8
1.15.5
Start-up procedure ..................................................................................................... 32
1.16
Installing the filter cartridges .......................................................................................... 32
1.16.1
The role of the filter cartridge ..................................................................................... 32
1.16.2
Filter cartridge care instructions ................................................................................ 33
1.16.3
Filter cartridge installation .......................................................................................... 33
1.16.4
Adding a filter cartridge .............................................................................................. 34
1.16.5
Assigning filter descriptions in Quansoft .................................................................... 35
1.16.6
Editing filter descriptions ............................................................................................ 35
1.16.7
Removing a filter cartridge ......................................................................................... 35
1.16.8
Points to remember ................................................................................................... 36
1.17
Changing the instrument name ...................................................................................... 36
1.18
LED power settings ........................................................................................................ 38
1.19
Inserting a plate .............................................................................................................. 38
1.19.1
Inserting a previously run plate .................................................................................. 38
1.19.2
Running a plate .......................................................................................................... 38
1.20
After use ......................................................................................................................... 39
2
Getting started .......................................................................................................................... 41
2.1
Introduction to PrimeQ ....................................................................................................... 43
2.1.1
Principle of a real-time PCR instrument .................................................................... 43
2.1.2
Principle of PrimeQ .................................................................................................... 43
2.1.3
Applications of PrimeQ .............................................................................................. 43
2.2
Introduction to Real-time PCR ............................................................................................ 44
2.2.1
PCR ........................................................................................................................... 44
2.2.2
Qualitative vs. real-time PCR .................................................................................... 44
2.2.3
Real-time PCR on PrimeQ......................................................................................... 45
2.3
Overview of fluorescent chemistries................................................................................... 45
2.3.1
Intercalating dyes ....................................................................................................... 46
2.3.2
Fluorescent labelled probes ...................................................................................... 46
2.3.3
Molecular Beacons .................................................................................................... 47
2.4
System overview ................................................................................................................ 48
2.5
Optics module..................................................................................................................... 48
2.5.1
The optical light path .................................................................................................. 49
2.5.2
Filter cartridges .......................................................................................................... 49
2.5.3
Adjustable LED intensity ............................................................................................ 49
2.6
Thermal cycling module ..................................................................................................... 49
2.7
Scanning module ................................................................................................................ 50
3
Quansoft ................................................................................................................................... 51
3.1
Introducing Quansoft .......................................................................................................... 53
3.2
Software overview .............................................................................................................. 54
3.2.1
Home page ................................................................................................................ 55
