155
•
Click
Next
and the Allelic Discrimination Wizard will launch.
4.9.2.1
Baseline correction
The baseline correction methods are the same as for plus-minus scoring in that the data should be
kinetic (i.e. > 1 reading) and that the Proportional baseline correction method is unavailable.
•
Choose the appropriate method and click
Next
.
4.9.2.2
Allele scoring method
The purpose of this part of the analysis setup is to define which readings should be used for allele
scoring. The options displayed are the same methods used as in plus-minus scoring.
•
End-point (default):
Uses the last reading only (we recommend that >1 reading is used for
accuracy).
•
All readings:
Averages all readings in the stage
•
Last readings
: Averages a user-specified number of last readings (the default is 5, or 1 if
there are less than 5 readings)
•
Specify range:
Specify a range of readings to be averaged that best suit the data (the
default is the last reading).
