10
4.3.1
Viewing the results ................................................................................................... 115
4.3.2
PrimeQ Report ......................................................................................................... 116
4.4
Passive reference dye (PRD) correction .......................................................................... 117
4.5
Analysis method: Baseline correction .............................................................................. 118
4.5.1
Assay requirements ................................................................................................. 118
4.5.2
Setup........................................................................................................................ 118
4.5.3
Viewing the results ................................................................................................... 120
4.5.4
PrimeQ Report ......................................................................................................... 122
4.5.5
Quick guide to baseline correction analysis ............................................................ 122
4.6
Analysis method: Quantification ....................................................................................... 123
4.6.1
Assay requirements ................................................................................................. 123
4.6.2
Setup........................................................................................................................ 123
4.6.3
Viewing the results ................................................................................................... 127
4.6.4
PrimeQ Report ......................................................................................................... 128
4.6.5
Using Cq values ....................................................................................................... 128
4.6.6
Comparing Cq values using a standard curve......................................................... 128
4.6.7
Comparing Cqs in relative quantification ................................................................. 132
4.6.8
Quick guide to quantification analysis ..................................................................... 137
4.7
Analysis method: Dissociation curve ................................................................................ 138
4.7.1
Assay requirements ................................................................................................. 139
4.7.2
Setup........................................................................................................................ 139
4.7.3
Viewing the results ................................................................................................... 145
4.7.4
PrimeQ Report ......................................................................................................... 146
4.7.5
Quick guide to dissociation curve analysis .............................................................. 146
4.8
Analysis method: Plus-minus scoring .............................................................................. 148
4.8.1
Assay requirements ................................................................................................. 148
4.8.2
Setup........................................................................................................................ 148
4.8.3
Viewing the results ................................................................................................... 150
4.8.4
PrimeQ Report ......................................................................................................... 152
4.8.5
Quick guide to plus-minus scoring analysis............................................................. 152
4.9
Analysis method: Allelic discrimination ............................................................................ 154
4.9.1
Assay requirements ................................................................................................. 154
4.9.2
Setup........................................................................................................................ 154
4.9.3
Viewing the results ................................................................................................... 156
4.9.4
PrimeQ Report ......................................................................................................... 159
4.9.5
Quick guide to allelic discrimination analysis........................................................... 159
4.10
Analysis method: Multi-read ......................................................................................... 161
4.10.1
Assay requirements ................................................................................................. 161
4.10.2
Setup........................................................................................................................ 161
4.10.3
Viewing the results ................................................................................................... 162
