85
•
Dissociation curve
•
Plus/minus scoring
•
Allelic discrimination
•
Multi-read
See Chapter 4 for a specific discussion of each analysis method in terms of requirements, setup
and what each approach can reveal about the experimental data.
3.4.5.3
Assigning a dye usage
As there may be more than one dye reading in a single stage, the role of each dye needs to be
assigned. To do this, the Analysis Wizard Selection box has a drop-down menu containing a list of
definitions for each. All definitions are treated as reporters apart from the passive reference dye
(PRD). When the analysis method is undefined or set to ‘none’ then the dye usage selection boxes
are disabled (greyed-out).
It is important to note that if an instrument is not connected and the experiment is not a template
from a previous run then dyes cannot be assigned until an instrument is connected.
•
Click
Next
to move through to the Analysis Wizard specific to the analysis method chosen.
3.4.5.4
Analysis wizards
Clicking
Next
from the Analysis Wizard Selection box branches the Analysis Wizard into the
individual wizards tailored to the particular method of analysis chosen. The parameters displayed
will vary according to the analysis method selected and these are discussed in detail in Chapter 4.
In general the Analysis Wizard will lead the user through the setup procedure as follows:
1.
Open the Analysis Wizard:
•
In the Analysis Selection box in the Experiment Editor, highlight the stage name and click
Edit
. The Analysis Wizard Selection screen will appear.
•
Choose the type of analysis required from the drop-down menu and assign a use for each
dye.
•
Click
Next
to open the selected Wizard. The Wizard screens have the following format:
