136
•
Dye to view options offer relative quantification cycles or individual reporter results and
graphs.
•
Changing the Dye to View from Relative Cq to a single reporter displays the baseline
corrected graph (if chosen in the setup) in addition to the quantification cycles graph for the
individual dyes.
Column headings:
•
No:
Well number.
•
Well ID:
Location of well.
•
Sample ID:
User-supplied or default name of well.
•
Cq (Dye 1):
Quantification cycle for Dye 1.
•
Cq (Dye 2):
Quantification cycle for Dye 2.
•
∆
Cq:
Calculated
∆
Cq.
•
∆∆
Cq:
Calculated
∆∆
Cq.
•
2
-
∆∆
Cq
:
Calculated 2
-
∆∆
Cq
•
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