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Summary of Contents for WX 100 Ultra

Page 1: ...50105750 4 October 2010 Thermo Fisher Scientific WX Ultra Series WX 100 WX 90 WX 80 Instruction Manual ...

Page 2: ...ific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsi...

Page 3: ...ting user ID code 2 9 Using Rotor Life Management 2 10 Basic operating procedure 2 11 Normal operation 2 13 Zonal operation 2 15 Acceleration and deceleration rates 2 17 How to use the FUNCTION field 2 19 Programmed operation 2 21 Step mode operation 2 32 RTC real time control feature 2 40 Displaying and setting RCF 2 48 Setting w2T 2 56 Defrost function 2 58 Setting and resetting zonal operation ...

Page 4: ...ndle 3 2 Cabinet 3 2 Replacement parts 3 2 Service decontamination policy 3 2 Chapter 4 Troubleshooting 4 1 Alarm indicators 4 1 Diagnosed problems requiring maintenance 4 3 Non diagnosed problems 4 3 Chapter 5 Preinstallation 5 1 Location Requirements 5 1 Electrical requirements 5 2 Leveling 5 2 Moving the ultracentrifuge 5 3 Chapter 6 Specifications 6 1 Chapter 7 Supply list 7 1 Appendix A Servi...

Page 5: ...nd critical instructions DANGER WARNING and CAUTION information is accompanied by a hazard symbol and appears throughout the manual both on the Important Safety Reminder pages and near the information it corresponds to Before you operate the centrifuge we recommend that you read this instruction manual thoroughly particularly all DANGERS WARNINGS and CAUTIONS Never operate the centrifuge without f...

Page 6: ...on Centrifuges routinely deal with high energy levels and could move suddenly in the unlikely event of rotor failure During centrifuge operation never lean on or move the centrifuge keep the surrounding area clear of objects including all hazardous and flammable materials and do not work on top of or next to the centrifuge as a rule keep people and objects at least 300 mm 12 inch away Never unlock...

Page 7: ...correctly assembled and properly seated on the centrifuge drive spindle before operation If using a swinging bucket rotor always make sure that a bucket is installed at each position and that each is properly seated and can swing freely after installing the rotor on the centrifuge drive spindle see rotor manual Do not exceed the maximum speed of the tubes bottles or adapters being used Check manuf...

Page 8: ...Preface Intended use and safety definitions Important safety reminder vi WX Ultra Series Thermo Scientific ...

Page 9: ... deck makes it easy to install and remove the rotor 9 Low noise level makes the ultracentrifuge suitable for use in any laboratory 10 Samples can be easily balanced visually 11 CFC free thermoelectric cooling system with greater cooling capacity Advanced features options The options of the Sorvall WX Ultra series ultracentrifuges are Compass Software Lockout Kit and a Printer The Compass Software ...

Page 10: ...me printed on front cover The following is the external view of the WX ultracentrifuge External view of ultracentrifuge Figure 1 1 External view of the Sorvall WX Ultra series Note This height is measured from level floor surface Top deck Door Safety cover Display panel 690 mm 27 2 in 790 mm 31 1in 850 mm 33 5 in 1000 mm 39 4 in Operation key Rotor rubber mat Power switch Front cover ...

Page 11: ...ays running conditions and running status this screen is called the Run Screen along with features such as Programmed Run Rotors List and User Customization Screens Fig 1 2 shows the display panel and Fig 1 3 represents the keyboard Figure 1 2 Display panel Figure 1 3 Keyboard 3 Message indicator 1 Field display 5 Vacuum indicator 4 RUN mode indicator 2 FUNCTION field Operation state display field...

Page 12: ...1 C Bottom row Sets a setting in the range from 0 C to 40 C in increments of 0 1 C ACCEL acceleration mode indicator Displays acceleration modes 1 through 9 DECEL deceleration mode indicator Displays deceleration modes 1 through 9 along with free coast F 2 Function field ID CODE Sets an ID code RLM Switches to the Rotor Management screen PROG RTC Sets recalls programmed runs or sets the time and t...

Page 13: ...nforma tion Rotor Catalog and User Customization Routines 11 Cursor key a Displays the cursor on the Run Screen putting the display into input wait status b Move the cursor on the screen 1 Moves the cursor up 2 Moves the cursor down 3 Moves the cursor to the right 4 Moves the cursor to the left 12 Numeric key Used to type numbers for setting run conditions During time and temperature entry Moves c...

Page 14: ...n incorrect detection Whenever the sensor is wet wipe it with a clean dry cloth Take care not to scratch the surface of the sensor Drive spindle Speed sensor Chamber door seal Handle Chamber door Temperature sensor measuring the temperature of the rotor Door lock system Vacuum chamber Bowl Rotor Chamber Thermoelectric cooling element cooling the rotor Guard Overspeed detector detecting any instanc...

Page 15: ...tles and rotor 3 Door lock system The chamber door automatically locks for safety while the rotor is spinning When the power supply is off the door remains locked The door can only be opened and closed when the rotor is at rest and the rotor chamber is vented Unless the door is closed the rotor will not start rotating except in zonal mode To open the door in the event of a power failure see Sectio...

Page 16: ...base has alternating black and white bands The number of bands corresponds to the maximum permitted speed of the rotor See Fig 1 5 Figure 1 5 Standard rotor To protect the overspeed decal be sure to store the rotor on the rotor stand provided See Fig 1 6 Figure 1 6 Rotor stand Overspeed decal Standard rotor with overspeed decal ...

Page 17: ...rammed operation Step mode operation More than one normal operation can be combined into a sequence of operations or step for successive centrifuga tion Section Step mode operation RTC real time con trol operation Run starts or completes at a required date and time Section RTC real time control feature RCF centrifugal force value display setting This feature calculates centrifugal force RCF values...

Page 18: ...pors or extreme exothermic reactions 2 When using radioactive toxic or pathogenic materials be aware of all characteristics of the materials and hazards associated with them in the event leakage occurs during centrifugation If leakage does occur neither the centrifuge nor the rotor can protect you from particles dispersed in the air To protect yourself we recommend additional precautions be taken ...

Page 19: ...ifuge Before setting run conditions display the run screen screen for setting run conditions 1 Displaying the Run Screen screen for setting run conditions Figure 2 1 Initial screen and run screen Initial screen 1 Turn on the POWER switch 2 The initial screen appears 3 The Run Screen appears ...

Page 20: ...of the rotor when partially filled The tube or bottle must be full in the following cases 1 When a thin tube or seal tube is used 2 When a thick tube is used for swinging rotor 3 When a bottle is used 100 000 x g or more Basic operation Setting run conditions This section will first describe the screen for basic operation the run screen and the cursor keys Within 5 mm 0 197 in Within 5 mm 0 197 in...

Page 21: ...he determined input state and the numerical part of the setting field will blink a 0 or numerical value and display the cursor In this state the system accepts a numerical input Press cursor keys to move the cursor To set a run condition enter the cursor into an input wait state move the cursor to the item you want to set then enter a value If you have made no keystroke such as a numerical input f...

Page 22: ...he setting thus making the system wait for a new input 3 If the system is running in HOLD and you want to set it to shut down at a future time enter a new time setting while the instrument is in operation enter the sum of the time elapsed plus the time remaining If for example this machine has run continuously for five hours and you want to stop it one and a half hours later use cursor keys to ent...

Page 23: ... numbers are moved to the left every time a new number is entered The last two digits are fixed Press the key to move the cursor to the minutes position For a continuous run press HOLD FREE 5 Make a check then press ENTER After pressing a cursor key you can still enter a setting similarly to the ENTER key Use CE to cancel an input Set it to 100 000 rpm Set it to 2 30 2 hours 30 minutes Setting ran...

Page 24: ...ree coast press HOLD FREE Display F Set it to 4 5 C Set it to 9 Set it to 7 Can be set to any value in the range from 0 to 40 C in increments of 0 1 C 1 to 9 1 to 9 free coast F FUNCTION field The FUNCTION field switches to the ID CODE field The number of units of ID CODE blinks The entered number is not displayed on the screen is displayed instead of the number After the registered ID CODE is ent...

Page 25: ...e user does not need to be identified the centrifuge can be operated without an ID CODE To use a name user name corresponding to the user ID code or ID code it must be registered For the registration method see Section User list Step Key operation Screen display and considerations 1 On the Run Screen use cursor keys to move the cursor to ID CODE and press the ENTER key 2 Use numeric keys to enter ...

Page 26: ...er set will appear as an undefined rotor in the displayed list Undefined rotor 1 You can use a particular rotor as undefined if you need to manage the rotor separately from the other defined rotors in terms of the number of runs made and accumulated run time Undefined rotor 2 For more information on rotor life management see Section Rotor management Step Key operation Screen display and considerat...

Page 27: ...run conditions Press VACUUM For information on how to set the run conditions see Section Basic operation Vacuum pump is ON Temperature control starts Check the set run conditions for accuracy Press START Rotor accelerates to the set speed and then high speed centrifugation begins Indicates panel key Zonal run Reaches the set time or press STOP Rotor comes to a stop Press VACUUM Temperature control...

Page 28: ...Check the set run conditions for accuracy This step may be skipped Just press START to run the ultracentrifuge When START is pressed the vacuum pump turns on and the rotor begins to accelerate Then the rotor stays at 4000 rpm until the rotor chamber reaches predetermined vacuum level Press START Rotor accelerates to the set speed Reaches the set time or press STOP Rotor decelerates to the zonal sp...

Page 29: ...ction manual carefully 4 Set run conditions See Section Setting run conditions and set run conditions 5 Press VACUUM key optional The machine starts evacuating the rotor chamber Temperature control starts The degree of vacuum in the rotor chamber is displayed on the vacuum indicator on the display panel If the rotor compartment has moisture or frost on it it takes a long time to reach a high vacuu...

Page 30: ...P key The rotor decelerates and stops 8 The rotor brakes or coasts to a stop Beeps to indicate that the rotor has stopped 9 Press VACUUM key The vacuum stops the air leak valve gets activates and the rotor chamber reaches atmospheric pressure The door unlocks and is able to be opened and closed 10 Take out the rotor Note Use the vacuum key to avoid prolonged vacuum wait time at 4 000 rpm when the ...

Page 31: ...eration to set speed and separation of the sample or 3 Centrifugation at zonal speed the sample and or gradient being unloaded in this stage Figure 2 8 Zonal operation mode Zonal speed The zonal speed is one that is required for loading and unloading the sample The zonal speed is normally set at 3 000 rpm but it can be set in the range from 2 000 to 3 000 rpm in increments of 100 rpm to obtain the...

Page 32: ...the VACUUM key This step may be omit ted Vacuum pump begins to work Chamber door is locked 10 Press the START key again Rotor begins to accelerate to the set speed Note The run time is counted from when the START key is pressed The instrument can also count only the time elapsing while the rotor is spinning at high speed This is possible by changing the run time setting range For details see Secti...

Page 33: ... assembly 15 Install the seal assembly and unload the sam ple 16 On the Run Screen move the cursor to ZONAL in the FUNCTION field and enter the following by operating numeric keys to set the normal operation mode Rotor decelerates to a stop Note During the zonal mode the rotor will not decelerate even if the STOP key is pressed during the rotor s spinning at the zonal sped 17 Be sure that the roto...

Page 34: ...nd deceleration Therefore the sample and gradient can become mixed especially in wide tubes if you use rapid acceleration or deceleration DNA separation by CsCl iso pycnic separation self forming gradients 9 7 You can operate at maximum acceleration because the density gradient is not formed during the run As for the deceleration it is better to decelerate slowly to obtain sharp bands Pelleting us...

Page 35: ... to use the FUNCTION field This ultracentrifuge incorporates a number of features These features are displayed and specified in the FUNCTION field The FUNCTION field is extended as shown in the following figure Figure 2 10 FUNCTION field Allowable maximum speed 500 rpm 2 minutes 15 minutes 15 minutes 2 minutes Speed ENTER key ENTER key ...

Page 36: ...rifuge at a desired date and time RCFmax Maximum centrifugal force for the maximum radius rmax of the rotor used This feature is used to cause the system to automatically calculate and display RCFmax It also sets an RCFmax value and calculates the speed RCFavg Causes the system to automatically calculate and display the average centrifugal force RCFavg for the average radius ravg of the rotor used...

Page 37: ...The saved run conditions will remain in effect even after the power switch is turned off This centrifuge incorporates the program areas indicated below It has twenty memory areas and nine steps in each memory number Accordingly twenty patterns of run conditions can be stored and each memory can store nine steps Running this machine with each memory number retaining multiple steps will allow you to...

Page 38: ...key Next page Press ESC several times to go back to the Run Screen Programmed operation possible Enter a memory number If you know which memory number stores the run condition Use cursor keys to select and press ENTER key To store change or delete a program or if you have forgotten the memory number where the run conditions are stored Press key to cause to blink and press ENTER key Enter a desired...

Page 39: ...the FUNCTION field Thermo Scientific WX Ultra Series 2 23 Note You cannot create change or delete a program while in running Perform these operations while not in running However you can search the memory screen every time ...

Page 40: ...ey 2 Press the upward cursor key to cause PROGRAM to blink then press ENTER key If you wish to store a new condition enter it into an empty memory number 3 Use numeric keys to enter a memory number you wish to store or change Example To store a condition in mem ory number 3 press 4 Select Creating or Changing The FUNCTION field switches to the PROGRAM field The MEMORY No setting field blinks The d...

Page 41: ...alog 2 Select Fixed Angle Rotor in the rotor types When using fixed angle rotor T 880 3 Select fixed angle rotor T 880 4 Select Yes The display switches to the Choose Rotor screen The display switches to the Used Rotor List screen You can search for the rotor number you need in the order starting with the latest date time The display switches to the Select Rotors screen The display switches to the...

Page 42: ...n conditions in multiple steps in a step mode operation continue the operation from the above See Section Step mode operation You cannot skip a step You cannot enter anything in step 2 with nothing entered into step 1 The display switches to the Rotor Management screen and the Rotor List is displayed The rotor type you have just entered appears in the top row in the screen The SPEED field in the S...

Page 43: ...ons select Yes When you press ESC key the bottom row message is displayed 1 A selection field as to whether User Name should be input or deleted appears 2 A selection field for selecting either inputting or deleting appears 3 Select Inputting User Name 4 Enter 4 digits of the ID CODE that is already registered 5 Check User name and select Yes 3 The display switches to the ID CODE input field 4 The...

Page 44: ...mark move the cursor to SET and press the ENTER key to register the character string 6 Check the contents of Remark and select Yes When you press ESC key the bottom row message is displayed 1 A selection field to input or delete Remark appears 2 A selection field to select either inputting or deleting appears 3 The display switches to the Remark registration field 4 Remark is entered from the left...

Page 45: ...ys the Program Map screen shown in process 3 The third push on ESC key switches you back to the Run Screen program screen The fifth push on ESC key switches you back to the Run Screen FUNCTION field Make sure that the condition is stored in memory number 3 Note 1 If you make and store changes in a memory area that already stores run conditions the previous conditions are replaced by the new condit...

Page 46: ... wish to operate Example to call memory number 3 press The message field in the bottom row displays PROG If the STEP field displays what is shown below this means that the memory unit you have just called stores multiple run conditions stepmode feature For details see Section Step mode operation The system only accepts numbers 1 20 5 Run the centrifuge under normal oper ation without making change...

Page 47: ...s the upward cursor key to blink PROGRAM Then press ENTER key 5 Use numeric keys to enter the memory number where you have stored data To call memory number 3 press If the contents of the memory number you have just recalled is not what you want press ESC to switch back to the Program Map screen and then call another memory unit number 6 If you get the memory number you want select Running The FUN...

Page 48: ... in process 5 Select a new condition 8 Start running the centrifuge under nor mal operation with no changes made to the run conditions Run this machine according to Section Normal operation If you make changes to the run conditions such as SPEED and TIME after recalling a program the program you have just recalled will be canceled You must recall the program again to use it The display switches ba...

Page 49: ...Operation How to use the FUNCTION field Thermo Scientific WX Ultra Series 2 33 Figure 2 13 A typical step mode run Run time Step 1 Step 2 Step 3 Speed rpm ...

Page 50: ...ER key 2 Press the upward cursor key to cause PROGRAM to blink then press ENTER key 3 Use numeric keys to enter the memory unit number you want to store To store data in memory number 3 press The FUNCTION field switches to the PROGRAM field The MEMORY No setting field blinks The display switches to the Program Map screen and the MEMORY No selection field blinks To store a new condition enter it in...

Page 51: ...rsor key downward after setting numeric value for DECEL D in step 1 setting the run conditions in step 1 finishes and step 2 enters into an input wait state 7 Then specify run conditions for step 2 The message field in the bottom row switches to what you see on your left hand side The display switches to the Choose Rotor screen The display switches to the SPEED priority setting field and the SPEED...

Page 52: ... 2 When attaching User Name to the memory run conditions select Yes 3 Select Inputting User Name 4 Enter 4 digits of the ID CODE that is already registered 5 Check User Name and select Yes When you press ESC key the bottom row message is displayed 1 A selection field as to whether User Name should be input or delete appears 2 A selection field for selecting either inputting or deleting appears 3 T...

Page 53: ...lect Yes When you press ESC key the bottom row message is displayed 1 A selection field to input or delete Remark appears 2 A selection field to select either inputting or deleting appears 3 The display switches to the Remark registration field 4 Remark is entered from the left 5 The display switches to the Remark check field 6 The display switches to the registration completion message field 12 P...

Page 54: ...calculates the total of running times of all steps of the programmed run and calculates the startup time for RTC You therefore cannot call a program memory number after setting RTC 14 To run the centrifuge select Yes Yes No If you select Yes The message field in the bottom row displays PROG If you select No the message field in the bottom row displays what you see in process 10 Make the selection ...

Page 55: ... use cursor keys to move the cursor to PROG RTC and press ENTER key Then move the cursor to PROG and press ENTER key 2 Press the upward cursor key to cause PROGRAM to blink then press ENTER key 3 Use numeric keys to enter the memory unit number you want to delete To delete memory unit 3 press 4 Select Deleting The FUNCTION field switches to the PROGRAM field The MEMORY No setting field blinks The ...

Page 56: ...n and display the SPEED alarm Double check the speed of all steps and correct any wrong ones 4 Stopping the centrifuge in operation Press STOP The rotor stops and the system does not move to the next step RTC real time control feature The Sorvall WX Ultra series ultracentrifuges contain an internal clock allowing you to run the machine at a specified start or finish time for centrifugation This fe...

Page 57: ...s example you set the above run conditions 2 through 6 set the finish time for RTC run to 9 30 a m October 1 and start the centrifuge You can make an identical setting by setting the start time to 5 30 a m instead of setting the finish time to 9 30 a m 100 000 Speed rpm START key ON 4 30 p m October 1 Automatic start at 5 30 a m October 2 4 h Stop around 9 34 a m Finish time 9 30 a m ...

Page 58: ...hen press Move the cursor to PROG RTC and press ENTER key 2 Move the cursor to RTC and press ENTER key 3 Select the item you wish to set To select the Setting stop time press Setting the Setting stop time Setting the Setting start time The display switches to the RTC Setup screen The dd item blinks mm and dd indicate the current date as a default If you do not make any input for 30 seconds the cur...

Page 59: ...n again 6 Check the RTC setting in the Run Screen then press START key The RTC run does not get activated unless you press START key Run this machine according to Section Normal operation Once the RTC setting is made you cannot change the run time centrifugation time If you wish to make a change to the run time cancel RTC first Pressing START enters the system into a DELAY and vacuum comes on cond...

Page 60: ...is more than 20 days after the current time Set the time to a time no more than 20 days afterwards 3 To change the run time centrifugation time after making an RTC setting cancel RTC and then set a new run time 4 To perform a combination of a programmed operation including a step mode operation with an RTC run call a program memory number then set RTC The system calculates the total run times of a...

Page 61: ...C run press STOP to abort the RTC first Press STOP to cancel the RTC run Step Key operation Screen display and considerations 1 1 While in the Run Screen use cursor keys to move the cursor to PROG RTC and then press ENTER key 2 Use cursor keys to move the cursor to RTC and then press ENTER key The message indicator displays RTC 2 Press cursor keys to blink RTC and then press ENTER key The FUNCTION...

Page 62: ...2 Perform process 4 and later ones in 1 How to perform an RTC run men tioned above and change the date and time settings See Section 1 How to perform an RTC run The screen switches to a screen where you can enter a stop time or start time Step Key operation Screen display and considerations 1 1 While in the Run Screen use cursor keys to move the cursor to PROG RTC and then press ENTER key 2 Use cu...

Page 63: ...un 1 Select Deleting the set RTC 2 Select Yes If you select Yes If you select No you will get the display you see in process 2 Make a selec tion again After starting the RTC run 1 Select Deleting the set RTC 2 i Select Cancel RTC NO run The message indicator in the bottom row changes The RTC setting has been canceled The message indicator in the bottom row changes ...

Page 64: ...l force value while setting an RCF value causes the centrifuge to automatically calculate and display the speed Given below is a description of how to display and set RCF 1 How to display an RCF value When the rotor to be used is already identified when rotor type is displayed in the message display field ii Select Cancel RTC Start run iii Select Continue RTC 3 Press ESC after process 2 i or ii Th...

Page 65: ...or to RCF ω2 T and press ENTER key 3 Move the cursor to RCFmax or RCFavg and press ENTER key The RCF value associated with the set speed you have just entered in process 1 appears in the RCF setting field The FUNCTION field switches to what is shown in the diagram to the left and the RCFmax field blinks The RCF display field displays the RCF value of the rotor RCF display field RCF setting field N...

Page 66: ...ations 1 Set the speed on the Run Screen See Section Setting run conditions 2 1 Use cursor keys to move the cursor to RCF ω2T and press the ENTER key 2 Use cursor keys to move the cursor to RCFmax or RC Favg and press the ENTER key 3 Check that ROTOR LIST blinks and press the ENTER key The contents of the display in the FUNCTION field change The RCFmax area blinks The FUNCTION field switches to th...

Page 67: ...e RCF value of the running rotor is displayed in the RCF display field When you select No the display goes to the same as the display of process 4 2 The display switches to the Used Rotor List screen You can search for the rotor number you need in the order starting with the latest date time The display switches to the Rotor List screen The fixed angle rotor list is displayed The specifications of...

Page 68: ... the upward or downward cursor key When using fixed angle rotor T 8100 3 Select Yes The RCF value of the running rotor is displayed in the RCF display field The display switches to the Rotor Management screen and the Rotor List is displayed The display switches back to the Run Screen and the selected rotor type is displayed in the message display field of the bottom row RFC display field ...

Page 69: ... in the Run Screen set a speed See Section Setting run conditions 2 1 While in the Run Screen use cursor keys to move the cursor to RCF ω2T and press ENTER key 2 Move the cursor to RCFmax or RCFavg and press ENTER key 3 Check that ROTOR LIST blinks and press the ENTER key The FUNCTION field switches to what is shown in the diagram to the left The RCFmax field blinks The FUNCTION field switches to ...

Page 70: ... value corresponding to the currently set speed is displayed in the RCF setting field Displayed at the initial time of no setting When you select No the display goes to the same as the display of process 4 2 Perform setting again The display switches to the Used Rotor List screen You can search for the rotor number you need in the order starting with the latest date time The display switches to th...

Page 71: ... and enter a desired centrifugal force Example RCFmaX 5000 x 100g Specify the RCF value to hundreds If you make no input for 30 seconds the cursor will disappear and you will be unable to make an input At that time press a cursor key The display switches to the Rotor Management screen and the Rotor List is displayed The display switches back to the Run Screen and the selected rotor type is display...

Page 72: ...To perform an ω2 T run set the ω2 T value instead of the run time Note 1 Press ESC key to cancel the RCF field 2 When you run this centrifuge at a speed determined based on an RCF value a slight rounding may occur of up to 2 between the set RCF value and the actual value because the speed setting is set in increments of 100 rpm Speed rpm Time ω2 T ω2 T run ...

Page 73: ... to move the cursor to the w2T setting field and enter a desired w2T value Example 9 87 x 1011 4 When you press START key the machine starts to perform an w2T run The contents of the display in the FUNCTION field change ω2T blinks The FUNCTION field switches to the ω2T field ω2T display field ω2T setting field The value you entered is displayed in the ω2T setting field The time value is calculated...

Page 74: ...to melt ice buildup so that the moisture can then be removed How to use the defrost function Defrost operation will heat the evaporator gradually and after the lapse of 10 minutes the defrost operation and vacuum ends automatically After the end of the defrost operation the DEF display in the message field disappears During the defrost operation DEF in the message display field in the bottom row o...

Page 75: ... 2 The temperature rise of the rotor due to defrost operations for 10 minutes should be 1 C or less 3 During rotor acceleration or at set speed defrost can be set but the defrost function is not available 4 If DEF is selected during accel or set speed it will not function until the run times out or stop is pressed Step Key operation Screen display and considerations 1 On the Run Screen use cursor ...

Page 76: ...eds up to a derated maximum speed of the rotor typically 90 of the initial maximum rated speed Rotor life management In order to comply with warranty requirements rotor use must be documented in a rotor log book provided with the ultracentrifuge Additionally the Sorvall WX Ultra series ultracentrifuges have the capability to keep track of the number of runs and hours of use for each rotor This inf...

Page 77: ...ultracentrifuge derates its maximum permitted speed and inserts at the right side of the rotor S N Once the maximum permitted speed is derated the actual speed of the rotor is automatically restricted within the new limits Automatic rotor exclusion When a registered rotor reaches the end of its life the ultracentrifuge indicates it by displaying a message on the screen Subsequent runs of this regi...

Page 78: ...tor List by using the left and right arrow keys 2 Use the cursor keys and to move the cursor to the line that is not yet registered New Rotor and press the ENTER key 3 Select Creating or Changing 4 Select Fixed Angle Rotor in the Rotor List screen and The display switches to the Rotor Management screen and the Rotor List is displayed The display switches to the Rotor List screen The display switch...

Page 79: ...er of runs in the Runs field Do not press the ENTER key 9 Enter the total number of hours in the Hours field by using numeric keys Do not press the ENTER key Note Enter this running time up to one decimal place in units of hour 1200 hours 24 minutes 1200 4 10 Press the ENTER key Specifications of the T 890 rotor are displayed The display switches to the Rotor Management screen You can enter a seri...

Page 80: ...be reentered from step 1 See the next page In the steps 7 to 9 it is possible to change the value repeatedly by moving the cursor with the arrow keys unless the ENTER key is pressed Note Once a registered rotor is deleted all of the life management data for the rotor will be lost Step Key operation Screen display and considerations 1 While in the Run Screen use the cur sor keys to move the cursor ...

Page 81: ...life management data for a standard rotor perform the following procedure Step Key operation Screen display and considerations 1 While in the Run Screen use the cur sor keys to move the cursor to RLM and press ENTER key 2 Use cursor keys to move the cursor to the T 890 line to be updated and press the ENTER key 3 Select Creating and Changing by pressing the following keys 4 Enter the updated total...

Page 82: ...re 1 Check that the rotor is at rest Listen carefully for any sounds coming from the drive 2 Then unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock 3 Remove the four M5 hexagon head bolts fixing the front cover from both sides and pull down the front cover by pulling its lower side forward Then remove the front cover The upper side of ...

Page 83: ...ame time push the door handle Then the door opens When opening the door confirm that the rotor is not rotating If it is still rotating immediately close the door 6 Remove the rotor After the removal close the air vent and put the front cover back to the ultracentrifuge Insert the top surface of the front cover in the bottom surface of the front side of the table and put the bottom surface of the f...

Page 84: ...h the front cover removed Figure 2 18 Installing the front cover 7 Turn on the power breaker Handle Vacuum release screw Vacuum chamber Door unlock wire Power breaker M5 hexagon head bolt for fixing the front cover 4 pcs WARNING When the centrifuge will not be used for a long time keep the power breaker off ...

Page 85: ...mber of the item you wish to use and press the ENTER key and the respective screen will appear Figure 2 19 Menu screen Centrifuge scheduler Scheduled centrifuge operation can be reserved 1 Reservation of scheduled centrifuge operation Step Key operation Screen display and considerations 1 On the Menu Screen select a centri fuge operation schedule 2 Use cursor keys to move the cursor to No you desi...

Page 86: ...is already regis tered 4 digit number 4 Select Reserve 5 Use cursor keys to move the cursor to the position at which you want to change the date time of reservation and enter a number by using numeric keys Note Enter a time later than the current time 6 At the desired date time of reserva tion press the ENTER key ID code The User Name corresponding to the ID Code is displayed In this example ABC l...

Page 87: ...ected 3 Enter the ID Code of the reserving per son proper 4 digit number 4 Select Delete 5 Select Yes The ID Code input field is displayed in the message field in the bottom row ID code When the ID Code is accepted the reserve delete selection message is displayed in the message field of the bottom row The confirmation message is displayed in the bottom row The No 3 reservation display disappears ...

Page 88: ...his number is not displayed when the centrifuge is operated The mark is displayed instead of the number User Name Character string of 8 digits or less The user name corresponding to the ID Code is displayed on the screen Alphabetical upper case and lower case characters and several types of special characters are available Note Registration of ID Code and User Name is not always required for opera...

Page 89: ...er the character string 6 Select Yes 7 Enter the ID Code 4 digits by using numeric keys and press the ENTER key v The User List screen switches to the User Name screen Screen for entry of characters The cursor is positioned at SET The confirmation message is displayed The ID Code input field is displayed in the message field of the bottom row ID code Enter the ID Code and press the ENTER key and t...

Page 90: ...e cursor and press the ENTER key Select No 9 3 Select Changing 4 Enter the ID Code used for registering No 9 by using numeric keys 5 Change the User Name according to the indication given on the screen The Menu Screen switches to the User List screen For a change select a registered No The inquiry message to ask what to do in the message field of the bottom row is displayed The ID Code input field...

Page 91: ...displayed together with its date of registration on the User List screen Note Deleting a User Name is limited to the person with the same ID Code who registered it Step Key operation Screen display and considerations 1 On the Menu Screen select User List 2 Use cursor keys to move the cursor to No 9 and press the ENTER key The User Name and ID Code of No 9 are deleted 3 Select Deleting The Menu Scr...

Page 92: ... this centrifuge you can use this screen to take immediate action For details see Section 4 Troubleshooting Figure 2 20 Alarm Information screen 4 Enter the ID Code used for registering No 9 by using numeric keys 5 Select Yes When you select No the display switches back to the screen display of Step 2 6 Press the ESC key The User List screen switches to the Menu Screen The confirmation message is ...

Page 93: ... is output to the printer printer optional The same identification number is also used as the centrifuge identification number for managing log data by using the operation log management program option on a PC The identification number is not factory set Unless you need it specially use it as it is Step Key operation Screen display and considerations 1 On the Menu Screen select Rotor Catalog 2 To ...

Page 94: ...ber of the desired language and press ENTER key 2 Changing from the Run Screen Normal Displays the Run Screen Graphics and zoom Twenty seconds after the actual speed reaches the set speed while in the Run Screen the display will automatically switch to the screen shown in Fig 2 23 or 2 24 The display will then return to the Run Screen if you press any key on the keyboard or while a deceleration sh...

Page 95: ...5 Contrast adjustment 3 Setting run parameters 1 Run Time display This function selects either Time elapsed or Remaining time as the time display in the run status When you select Time elapsed lapse is displayed in the time display frame on the screen When you select Remaining time rem is displayed in the same frame Figure 2 26 Run Condition setting screen Note To get back to the Run Screen press ...

Page 96: ...select one of the following values as the degree of vacuum in the process from the vacuum wait to accelerationto the set speed Medium vacuum Approx 133 Pa ordinary High vacuum Approx 13 Pa Figure 2 28 Vacuum waiting operation mode Note The vacuum waiting time is not counted in the normal operation To eliminate the run time difference in the high vacuum start mode vacuum the rotor chamber up to a h...

Page 97: ...aintained in the ultracentrifuge on the optional printer The information includes run records rotor usages memory contents etc When you select 5 PRINT UTILITIES from the user customization routines submenu this submenu option starts working and displays the PRINT UTILITIES screen Note When Unused is selected in the optional unit setting machine log data will be deleted in the order starting with t...

Page 98: ...hine Log 1 Select the corresponding item number on the Print Utilities Menu screen 2 Rotor Management 3 Rotor Log 1 On the Print Utilities Menu screen select 3 Rotor Log 2 On the Rotor Management screen move the cursor to select a rotor and press the ENTER key 3 Select 1 Yes as print permission 4 Set run parameters 1 Select the corresponding item number on the Print Utilities menu screen 5 Run res...

Page 99: ... select 8 User Log 2 Move the cursor to select a User Name and perform printing 9 Machine Management 1 On the Print Utilities menu screen select 9 Machine Management 10 Record after each run 1 On the Print Utilities menu screen select the corresponding item number 2 Move the cursor and select a setting condition 11 Printout after each run The display switches to the Program Map screen The display ...

Page 100: ...runs can be printed out at a time Once the ultracentrifuge run records are printed out they are gone Therefore if necessary save the produced printout for future reference 2 RUN RECORDS OF ALL ROTORS Run records of all the rotors registered are printed out When a rotor reaches its primary life an asterisk is dis played at the right of the maximum speed and the maximum rated speed is derated by 10 ...

Page 101: ...rds of which you want to print out and then press the ENTER key Once the run records of the run are printed out they are gone Therefore if necessary save the produced printout for future reference 4 SET RUN PARAMETERS PRINTOUT The currently set run parameters are printed out 5 RUN RESULT PRINTOUT The result of the last run made is printed out 6 RUN CONDITION LOG OUTPUT The up to date run log is pr...

Page 102: ...ge user s run log is printed out up to 20 runs Once the user log is output the previous run record is deleted and cannot be printed out once again 9 MACHINE MANAGEMENT OUTPUT The running time and the total number of revolutions are totaled for each user Regarding the total number of revolutions the ratio of each user in the total number of revolutions is calculated and printed out Totalization is ...

Page 103: ...on is toggled off ultracentrifuge runs are not recorded in the run record buffer See page 2 79 Therefore even if the 40th run in a sequence is completed the run records including the 40th will not be automatically printed out Contents of error Process Executing the following processing permits printing out from the beginning 1 Centrifuge power OFF The record is left in the centrifuge memory After ...

Page 104: ...User s name press enter after each letter Then use down cursor key to SET press ENTER 9 Press ENTER Yes register this User Name 10 The number 1 is highlighted press ENTER 11 Input ID Code 4 digits then press ENTER 12 Input Password then press ENTER 13 Repeat step 6 thru 10 to input additional users 14 Once completed hit ESC until Run Screen appears move cursor key to Logoff then press ENTER Note W...

Page 105: ...aged replace it Wipe the groove for the door seal o ring with a clean soft cloth dampened with alcohol or a similar solvent DANGER Before removing the cover top deck or other components for maintenance be sure to turn off the POWER switch of the centrifuge Then unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock WARNING 1 Be sure to take...

Page 106: ...o personnel either at the customer s laboratory or at a Thermo facility comply with the following procedure to ensure the safety of all personnel 1 Clean the centrifuge to be serviced of all encrusted material and decontaminate see Maintenance Section of centrifuge it prior to servicing by the Thermo representative or returning it to the Thermo facility There must be no radioactivity detectable by...

Page 107: ...be serviced does not have a Decontamination Information Certificate attached and in Thermo s opinion presents a potential radioactive or biological hazard the Thermo representative will not service the equipment until proper decontamination and certification is complete If Thermo receives a centrifuge at its Service facilities which in its opinion is a radioactive or biological hazard the sender w...

Page 108: ...ifuge Then unplug the power cord from the wall outlet and wait at least three minutes to avoid the risk of electrical shock WARNING 1 Be sure to take necessary safety measures before using materials which are toxic radioactive or contaminated with pathogenic micro organisms 2 If the instrument the rotor and or accessories has been used with toxic radioactive or pathogenic materials clean it by fol...

Page 109: ...screen To get back to the Run Screen press ESC key twice Alarm Cause Action DOOR 1 The VACUUM or START key has been pressed with the chamber door left open Shut the door completely and press VACUUM or START key VACUUM 1 Required level of vacuum cannot be reached 2 After a satisfactorily high level of vacuum was reached it lowered due to for instance sample leakage Wipe off the moisture from inside...

Page 110: ...ing Unless the set run time has elapsed restart the run If the power was automatically restored and the rotor is rotating at set speed then let the run continue Refer also to Section When power failure occurs Note When the No 13 Unexpected MPG pulse alarm occurs the related problem code is impossible to clear until the rotor stops Symptom Cause Remedy The ultracentrifuge cannot be energized by tur...

Page 111: ... cm or more behind the centrifuge The operating temperature range is 10 to 35 C If the room temperature rises above 35 C the temperature of the rotor may become too high Avoid installing the ultracentrifuge in direct sunlight or near any other heat generating machine equipment which might reduce the ultracentrifuge cooling capacity Figure 5 1 Place of installation CAUTION An authorized Thermo Fiel...

Page 112: ... For connection to other receptacles the power cord may need replacement Follow local electrical codes The centrifuge has a 20 A circuit breaker ON OFF power switch A separate line disconnect switch is not needed unless required by local code Leveling 1 Turn the four leveling screws with a wrench to lift the caster about 10 to 20 mm 0 4 to 0 8 in off the floor as shown in Fig 5 2 2 Turn on the POW...

Page 113: ...When moving the ultracentrifuge disconnect the power cord and unscrew the leveling screws with a wrench to lower the caster to the floor Raise the leveling screws enough to remove the leveling pads then move the ultracentrifuge After moving be sure to install and level the instrument again Level Drive shaft CAUTION Before moving the ultracentrifuge be sure to remove the rotor from the drive shaft ...

Page 114: ...mate vacuum below 0 01Torr 1 33 Pa This value reaches within 20 minutes after the rotor starts rotating Noise level 53 dB A scale measured 1m in front of the instrument at the set speed of the rotor Maximum heat dissipation into room 1kW hr Cooling method Thermo module cooling CFC free User interaction means Color liqiud crystal display color 256 touch panel and keyboard Interface RS232C X 1CH Dim...

Page 115: ... Sorvall WX Ultra series centrifuges satisfy CE marking requirements The CE marking is an international symbol which shows that the product conforms to EC directives Standards concerning these directives are as follows Product Safety EN 61010 1 and EN 61010 2 020 Electromagnetic compatibility EN 61326 1 ...

Page 116: ...isted below are those supplied with the ultracentrifuge Item name Part no Q ty Item drawing Remarks Instruction manual 45912 1 Vacuum grease 65937 1 Vacuum pump oil 45128 1 Supplied in 1 liter NEO VAC containers MR100 M5 screw 3 Hex bar wrench 1 Rotor rubber mat 1 ...

Page 117: ...Supply list 7 2 WX Ultra Series Thermo Scientific Optional items Item name Part no Q ty Item drawing Remarks Funnel 45215 1 Vinyl tube 45214 1 Cleaning bar 1 Door seal O ring 45664 1 ...

Page 118: ...f Certificate is not available attach a written statement verifying decontamination what was the contaminant and what decontamination method was used If the centrifuge must be returned to a Thermo facility 1 Contact your Thermo representative to obtain a Return Service Order Number RSO No be prepared with the name and serial number of the centrifuge or rotor and the repairs required 2 Send item s ...

Page 119: ...l Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Electron has contracted with one or more recycling disposal companies in each EU Member State and this product should be disposed of or recycled through them Further information on Thermo Electron s compliance with these Directives the recyclers in your country and information on Thermo Electron products...

Page 120: ... enter a new setting Entered numbers are moved to the left every time a new number is entered Use the cursor keys not the ENTER key to move between fields as run conditions are entered 2 After all conditions are set press ENTER Use CE to cancel an input 3 Press START When START is pressed the run mode indicator in the bottom right corner will begin to blink ACCEL denoting acceleration Once top spe...

Page 121: ... M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydroxide conc U U U U S U M S S S U S U U S S...

Page 122: ...U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S M S S M Acetic Acid 60 S S U U S S U S M S U U M U S M S M S M S M U S M U Ethyl Acetate M M U U S S M M S S U S U M U U S S U U S M M S U U Ethyl Alcohol 50 S S ...

Page 123: ... S S S S S M S S S S S S S M S S S Calcium Hypochlorite M U S M M S M S S M S S S S M S M U S S Kerosene S S S S S S U S M U S U M M S M M M S S U S S U S Sodium Chloride 10 S S S S S S S S S S S S S S S S S S M S Sodium Chloride sat d U S U S S S S S S S S S S S S S M S Carbon Tetrachloride U U M S S U M U S U U S U M U S S M M S M M M M U S S Aqua Regia U U U U U U U U U U U S M Solution 555 20 ...

Page 124: ...S Oleic Acid S U S S S U U S U S S M S S S S S S S S S M U S M M Oxalic Acid U U M S S S U S S S S S U S U S S S S S S S S U M S S Perchloric Acid 10 U U S U U S M M M U M S M M M S U S S Perchloric Acid 70 U U U U U S U M U U M U U U M M U M S U U S U S Phenol 5 U S U S M M S U M U U S U M S M S U U S U M M M S Phenol 50 U S U S U M S U M U U U U U S U M U U S U U U M S Phosphoric Acid 10 U U M S...

Page 125: ... U Trichloroethane S U M U U S U U U U U U U U U S U S S Trichloroethylene U U U U S U U U U U U U U U S U U S Trisodium Phosphate S M S S S S S S S Tris Buffer neutral pH U S S S S S S S S S S S S S S S S S S S S S S S S S Triton X 100 S S S S S S S S S S S S S S S S S S S S S S S S S Urea S U S S S S S S S M S S S S S S S M S S Hydrogen Peroxide 10 U U M S S U U S S S U S S S M U S S S S S S M S...

Page 126: ...terials under the stress of centrifugation when in doubt we recommend pretesting sample lots Key S Satisfactory M M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc suggest testing under actual conditions of use U U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable material ...

Page 127: ... of all the facts concerning operating conditions and other pertinent factors and after such component or accessory has been investigated and or factory inspected by Thermo Fisher Scientific 2 All items claimed defective must be returned to Thermo Fisher Scientific transportation charges prepaid and will be returned to Buyer with transportation charges prepaid Thermo Fisher Scientific will be rele...

Page 128: ...made by any person including dealers and representatives of Thermo Fisher Scientific which are inconsistent or in conflict with the terms of this warranty including but not limited to the limitations of the liability of Thermo Fisher Scientific as set forth above shall not be binding upon Thermo Fisher Scientific unless reduced to writing and approved by Thermo Fisher Scientific NO CLAIM OF ANY KI...

Page 129: ...RMO 1 866 984 3766 Canada 1 866 984 3766 Austria 43 1 801 400 Belgium 32 2 482 30 30 Germany 08001 536 376 49 6184 90 6940 France 33 2 2803 2180 Italy 39 02 02 95059 434 254 375 Netherlands 31 76 571 4440 Nordic Baltic Countries 35 89 329 100 Russia CIS 7 812 703 42 15 ...

Page 130: ...09 18 Portugal 34 932 23 09 18 Switzerland 41 44 454 12 12 UK Ireland 44 870 609 9203 China 86 21 6865 4588 86 10 8419 3588 India 1800 22 8374 91 22 6716 2200 Japan 81 45 453 9220 Other Asian Countries 852 2885 4613 Other Countries 49 6184 90 6940 33 2 2803 2180 ...

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