Roche Nimblegen MS 200, Operator'S Manual

Page: 87 / 104

NimbleGen MS 200 Microarray Scanner Operator’s Manual v1.0 | 75
Problem/Error Possible Cause(s) Corrective Measure(s)
Shift/misalignment of
pixels was apparent in
the image.
Instrument needs
recalibration.
Switch off then on power to the
Microarray Scanner and wait for
initialization to complete. Run the
ILC test to recalibrate the
instrument. If all test points pass
successfully, scan a test slide to
verify that pixel shift has been
corrected.
If the problem persists, contact
Roche Microarray Technical
Support.
Processing-Level Troubleshooting
Autofocus procedure
failed.
Slide does not match
selected slide type.
Verify the slide matches
predefined slide type options.
Slide is not present. Verify the slide is present in the
slide magazine.
Particles, dust, or
finger prints are
present on the slide.
Check the slide surface. If
possible, remove dust and/or
particles from the slide by
appropriate methods. (For
NimbleGen arrays, refer to the
NimbleGen Arrays User’s Guide
for instructions.) Repeat scan. If
the problem persists, run the ILC
test.
If the problem persists, contact
Roche Microarray Technical
Support.
The array image
appears too bright
after applying
autogain.
Autogain area is not
entirely located with a
feature area.
Place the autogain area in a
feature area. Refer to page 56 for
instructions on how to specify the
autogain area. Repeat scan.
Dust or particles
within the autogain
area affect the
autogain procedure.
Check the slide surface. If
possible, remove dust and/or
particles from the slide by
appropriate methods. (For
NimbleGen arrays, refer to the
NimbleGen Arrays User’s Guide for
instructions.) Repeat scan.