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NimbleGen MS 200 Microarray Scanner Operator’s Manual v1.0
6.4 Specifying the Autogain Area
STOP
The autogain area cannot be less than 3 x 3mm or greater than 22 x
22mm.
Make sure that the autogain area is located on a feature area when
determining PMT gain values automatically. Do not place the
autogain area offset between or outside of the feature area to avoid
too high or too low PMT gain values.
1.
If necessary, highlight the slide row in the Magazine Control (Figure
24).
2.
If necessary, open the Parameter Control (Figure 25).
3.
If necessary, open the Area Definition Control (Figure 29).
4.
Ensure the
Autogain area
checkbox is selected.
5.
Position the mouse pointer inside the respective rectangle and click to
enable the move cursor.
6.
Drag the rectangle to the desired location. As a recommendation,
ensure the rectangle is approximately 10mm from the edge of the
default scan area.
7.
Size the rectangle by dragging the side and corner handles. The
following figure provides guidance as to the size of and where to locate
the autogain area for NimbleGen arrays.
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