Roche Nimblegen MS 200, Operator'S Manual

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NimbleGen MS 200 Microarray Scanner Operator’s Manual v1.0 | 63
To automatically adjust the contrast to enhance the image:
Click Best Fit to automatically adjust contrast values for better
discrimination between the brightest signal and the darkest background
pixels of the image.
To manually adjust gamma, brightness, and contrast of the image:
Use the Gamma , Brightness , and Contrast sliders to change the settings.
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The Gamma slider adjusts the linearity of the displayed pixels to the
scanned intensity.
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The Contrast slider adjusts the difference between dark and bright
pixels.
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The Brightness slider adjusts the overall displayed intensities.
Adjusting the gamma, brightness, and contrast settings does not
modify the pixel values in the saved image. They only adjust the way
in which the image is displayed.
8.5 Displaying a Histogram or Line Profile
Using the Image Control (Figure 35), you can generate histograms or line
profiles. Histograms show an intensity frequency profile for a specified
region. Line profiles show an intensity profile for a specified vertical or
horizontal section.
To display a histogram:
Click Show Histogram to open the Histogram window (Figure 36 and
Figure 37). The histogram shows the pixel’s intensity distribution of the
currently selected area in the Slide View. The displayed color on the
histogram corresponds to the selected channel color. You can zoom the
boundaries of the histogram range by dragging the slider bars in the
histogram window.
Linear and Logarithmic Base 10 option buttons allow
you to select the scale on the Y axis for the histogram.