Molecular Devices QPix 450, User Manual

Page: 54 / 257

QPix 450 or QPix 460 Colony Picking System User Guide
54 5031498 G
3. From the Stages list, if needed, select the wash stage that you want to edit, and click Edit.
In the Steps area, the Add and Remove button are available in Edit mode.
To add a new wash-bath step, click Add to add the new step to the end of the list.
To delete a wash-bath step, select the step and then click Remove.
4. From the Bath Name list, select the wash bath to be used for the step. See Setting Up Wash
Baths on page 29 .
WashBath1—furthest from front, typically 70% ethanol in water.
WashBath2—middle wash bath, typically sterile water.
WashBath3—closest to the front, typically 1% sodium hypochlorite (bleach).
5. From the Bath Cycles list, enter the number of times to wash the picking pins for that step.
6. From the Dry Time (seconds) list, enter the number of seconds to dry the pins with the
halogen dryer.
7. From the Wait After Drying (seconds) list, enter the number of seconds to wait before
moving on to the next step, or ending the wash routine if this is the last step.
8. Continue to add or remove steps as required for the wash stage.
9. When you are finished editing, click Save.
Note: If you are editing a Multi Stage profile, make sure that you define wash steps
for both the First Wash Cycle and the Main Wash Cycle.
10. When you have finished editing and saving the wash-bath steps, click Next to return to the
Navigation window.
The edited Sanitise profiles are available for all processes.
Note: Before running a process that uses the wash baths, make sure that you verify the
locations and solutions of the wash baths and also the wash cycles that are required for
their use. Always top off your wash baths with their defined cleaning fluids and then wipe
down the surfaces of the instrument interior.