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4-2

Matec Applied Sciences

56 Hudson St., Northborough, MA 01532 Ph: (508) 393-0155 Fax: (508) 393-5476

www.matec.com

e-mail: [email protected]

4 Operating the CHDF 2000

This chapter describes how to perform normal CHDF 2000 operations.

4.1 Priming the Pump

Prime the pump to remove air bubbles from the eluant supply tubes or to purge eluant from the
system before you use new eluant.

To prime a pump

From the Home screen, choose Prepare system. The Prepare System screen appears as
shown in Figure 4.1.

Figure 4.1 The Prepare System Screen

Choose Prime pump. The Prepare System...Prime Pump screen appears (Figure 4.2).

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Summary of Contents for CHDF 2000

Page 1: ...l service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE INSPECTION Remotely inspect equipment before purchasing with our interactive website at www instraview com LOOKING FOR MORE INFORMATION Visit us on the web at www artisantg com for more information on ...

Page 2: ...ing the Control Panel I 11 Chapter 2 Theory and Sample Preparation 2 1 Scope II 1 2 2 Safety Precautions II 1 2 3 Intended Use II 2 2 4 Description and Specifications II 3 2 5 Theory of Operation II 7 2 6 Data Analysis II 8 2 7 Sample Preparation II 8 2 8 Eluant II 9 Chapter 3 Setting Up the CHDF 2000 3 1 Performing Power On Procedures III 2 3 2 Reviewing and Editing Operating Parameters III 7 Art...

Page 3: ...g to Error Messages V 1 5 2 Quick Troubleshooting Checklists V 4 5 3 Running CHDF 2000 Diagnostics V 8 Chapter 6 Maintaining the CHDF 2000 6 1 Maintaining the Pump VI 2 6 2 Maintaining the Manual Injector Valve VI 12 6 3 Maintaining the Priming Valves VI 16 6 4 Maintaining the Detector VI 17 6 5 Adjusting the Control Panel Display VI 24 6 6 Dealing with Plugged Cartridges VI 25 Artisan Technology ...

Page 4: ... MA 01532 Ph 508 393 0155 Fax 508 393 5476 www matec com e mail colloid matec com Matec Applied Sciences CHDF 2000 Hardware Users Guide Version 3 8 April 26 2006 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 5: ...plied Sciences 56 Hudson St Northborough MA 01532 Ph 508 393 0155 Fax 508 393 5476 www matec com e mail colloid matec com Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 6: ...exact time for every run No In both manual and auto sampler operation the software detects when the marker is injected The injection time is recorded the marker elution time is calculated as the marker exit time minus the injection time A marker delay time of zero seconds is recommended for auto sampler operation this is set under the current method file 6 How should the marker fluid be prepared T...

Page 7: ...s from samples prior to injection Syringe filters may only be used once per sample Marker as well as Rinse filters may be reused until leaks are observed on the filters 10 No peaks are detected in the Raw Data graph upon sample and marker injection The fractionation cartridge is likely plugged Refer to the troubleshooting section of the hardware manual for cartridge cleaning instructions 11 The pu...

Page 8: ...System Configure Auto Sampler 20 Are samples loaded directly into the fractionation capillary No They are loaded into a sample loop a piece of stainless steel tubing which is connected to a vent As sample is pushed into the injection valve the sample loop becomes filled with sample Sample exits from the sample loop vent as more sample is loaded The sample loop becomes connected to the pump and the...

Page 9: ...the bottom of the Text Report This list contains a description of all the particle size populations also referred to as modes automatically detected by the software in the sample Each mode is listed by the start and end particle size The percentages by Number Area and Weight are printed The Area is the average particle size of each mode Weight Weighted The mode detection sensitivity can be changed...

Page 10: ... a sigmoidal or polynomial fit Both options have sigmoidal shapes 33 What is Particle Frequency in the PSD graphs Particle Frequency and Volume Percentage are different The CHDF produces relative PSD s An absolute PSD shows the actual particle volume percentage However absolute PSD s are sometimes difficult to compare between different samples overlay The reason is that samples with different perc...

Page 11: ...08 393 0155 Fax 508 393 5476 www matec com e mail colloid matec com 34 How can the percentage of a particle size population be calculated See Particle Frequency question above Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 12: ... Hydrodynamic Fractionation CHDF to measure particle sizes from 15 nanometers to 1 1 microns in diameter Figure 1 1 CHDF 2000 The CHDF 2000 provides the following features Simple front panel control of all system operations An eluant delivery system UV absorbance detection Reduced workbench space requirements Control panel Front door Artisan Technology Group Quality Instrumentation Guaranteed 888 ...

Page 13: ...ntrol Panel The CHDF 2000 control panel Figure 1 2 provides a simple icon based interface that allows you to directly configure operate and troubleshoot the CHDF 2000 The control panel is located on the CHDF 2000 front door Refer to Section 1 3 Using the Control Panel for detailed instructions on using the CHDF 2000 control panel Figure 1 2 Control Panel and Manual Injector Valve Manual injector v...

Page 14: ... the front door Figure 1 3 A priming valve is connected to the pump outlet and is mounted directly below the manual injector valve Figure 1 3 Eluant Pump and Fractionation Cartridge Fractionation Cartridge In the CHDF2000 a capillary column provides hydrodynamic separation of the sample Instructions for installing the column are provided in Chapter 6 Absorbance Detector The CHDF 2000 includes a mu...

Page 15: ... A Pentium processor and Windows 95 are recommended for the personal computer Signal Connector Module 1V AU OUT PRESS OUT B OUT INJ OUT START IN The signal connector module Figure 1 5 on the CHDF 2000 rear panel provides signal connections for external devices such as a chart recorder or integrator You can also use these signals to synchronize the operation of the CHDF 2000 with other devices Figu...

Page 16: ...ation Figure 1 6 Fluidic Diagram of Operation Tubing ID Legend Waste outlet Strain gauge pressure transducer In line filter Pulse dampener Pump A 0 009 SS 0 020 SS 0 040 SS 0 062 TFE 20 µL loop Unions Manual injector valve 7725i Drain tubes SSI priming valve Optics bench Pump A solvent reservoir Whatman filter optional Pump A Eluant Reservoir Artisan Technology Group Quality Instrumentation Guaran...

Page 17: ...F 2000 control panel provides easy control of system configuration operation and diagnostic functions The control panel components Figure 1 7 include Control panel display System controls Figure 1 7 Control Panel Components Selector knob Keypad Control panel display Function keys Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 18: ...y the specific program screen Screen body The main body of each screen contains one or more of the following types of information Control options including system tasks and navigation options Status of current operation Instructions Secondary options or instructions Secondary options or instructions appear at the bottom of most screens Secondary options allow you to choose alternate navigation or ...

Page 19: ...am screen Selector knob Rotate to change highlighted screen option or to increase or decrease a value Press to select a highlighted option or to accept a displayed value 1 3 3 Moving Within and Between Screens The default option for a screen is highlighted when the screen first appears Moving Among Screen Options To highlight an option on the screen rotate the selector knob to move the cursor from...

Page 20: ...eter 2 Press the selector knob to accept the screen option or parameter or Press OK on the keypad 1 3 5 Entering Parameter Values You can enter parameter values by using either the selector knob or the keypad Using the Selector Knob To enter a value using the selector knob 1 Choose the parameter you wish to change 2 Rotate the selector knob to change the parameter value 3 Press the selector knob t...

Page 21: ... PRECAUTIONS 2 2 1 Electrical The pump and the detector in the CHDF 2000 use high voltage Avoid contact with any high voltage area Un plug the entire CHDF 2000 before performing service or maintenance Do not store or spill eluant or samples on the electrical modules Be certain that all laboratory power receptacles are properly grounded The CHDF 2000 uses ultraviolet radiation Avoid eye contact wit...

Page 22: ...tion cartridge offers resolution power two to three times higher than a standard resolution cartridge but at a lower upper particle size range 700 nm The C 203 cartridge measures particles in the range 15 nm to 1100 nm and has about half the resolution of the C 202 cartridge The C 203 provides particle detection sensitivity three to four times that of the C 202 cartridge High sensitivity may be ne...

Page 23: ...he particle size separation takes place in the top cartridge This cartridge contains a coil of glass capillary tubing as seen in Figure 2 2 This is the cartridge that needs to be replaced on occasion The back cartridge contains two glass capillary coils which rarely need replacement A large proportion of the eluant and sample flow through the back cartridge and then to waste Only a small portion o...

Page 24: ...s or exceeds the specifications given above 2 4 2 CHDF 2000 UV Detector Specifications _____________________________________________________________________________ 1 Spectral bandwidth 7 nm _____________________________________________________________________________ 2 Wavelength accuracy 2 0 nm _____________________________________________________________________________ 3 Wavelength repeatabili...

Page 25: ...ge of the standard C 202 Cartridge and the 2XGR 500 eluant is 15 nm to 1100 nm particle diameter A 15 nm to 700 nm high resolution range is available C 201 A high sensitivity cartridge for measurement of non UV absorbing materials is available C 203 2 Size Resolution C 202 Standard Resolution Cartridge Particle ratio 1 1 2 High Resolution C 201 cartridge Particle ratio 1 1 1 minimum C 203 cartridg...

Page 26: ...ductivity must be in the range 14 18 µSiemens cm The 1X GR500 conductivity should be between 6 and 10 µSiemens cm Contamination of the eluant especially by electrolytes will result in lower than expected particle sizes The same eluant type must be used during calibration and regular operation of the cartridge Changing the eluant type changes the calibration curve To prepare a batch of eluant combi...

Page 27: ...tube will sample all accessible radial positions if the particle elution time is sufficiently long The closest approach of the particle center to the inner wall of the capillary will be limited to a distance equal to the particle radius As the particle size increases it will become increasingly unable to sample the slowest moving eluant streamlines near the capillary wall As a consequence of this ...

Page 28: ...measured by CHDF will be an approximate spherical diameter Distributions in size will still be absolutely determined but no specific information will be given on the aspect ratio of the particles 2 7 SAMPLE PREPARATION The CHDF 2000 requires 1 milliliter of sample at 0 1 5 volume The sample must be aqueous based The sample can be prepared in either of two ways dilution of a concentrate or the addi...

Page 29: ...0 concentrate GR 500 should be diluted with de ionized water and the final solution should be filtered under vacuum through either a 0 1 or 0 2 µm pore size filter The purpose of this filtration under vacuum is to remove any particulate matter and also to de gas the GR 500 Care should always be taken when handling the GR 500 to avoid contamination The eluant should be used within 3 days of prepara...

Page 30: ... Hudson St Northborough MA 01532 Ph 508 393 0155 Fax 508 393 5476 www matec com e mail colloid matec com Chapter 3 Setting Up the CHDF 2000 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 31: ...o prevent injury apply power to the CHDF 2000 only after you have completed all installation tasks properly When you first power on the CHDF 2000 perform the following procedures Check power on diagnostics Check the Control panel display 3 1 1 Powering On the System To apply power to the system 1 Insert the power cord into the power outlet of an appropriate power source 2 Move the power switch to ...

Page 32: ...of program memory erasable programmable read only memory RAM Test Tests the validity of programmed method data saved in RAM random access memory Cartridge Heater Determines if an optional cartridge heater is installed Calibrate UV Performs calibration and check of the absorbance detector optics This test takes 2 to 3 minutes to complete If your CHDF 2000 fails any of the power on diagnostics refer...

Page 33: ...the Power On Diagnostics screen see Figure 3 2 press the selector knob to choose Continue The Home screen appears Figure 3 3 Figure 3 3 The Home Screen You are now ready to set up the CHDF 2000 for normal operation Preparing the System for Use From the Home screen see Figure 3 3 choose Prepare system The Prepare System screen appears as shown below Artisan Technology Group Quality Instrumentation ...

Page 34: ...es on the screen and pressing the selector knob performs the highlighted action When any of these four options are selected a Configure System screen appears as shown in Figure 3 5 Figure 3 5 The Configure System Screen Although there are 4 choices in this menu only one of them can be accessed at a time For example if you selected Flow Rate from the menu in Figure 3 4 when the menu in Figure 3 5 a...

Page 35: ...HDF 2000 You will need three buffer solutions typically of pH 4 0 7 0 and 10 0 to calibrate the pH sensor The sensor is calibrated by alternately immersing the sensor in the three buffers then entering the pH value of the buffer into the instrument This is accomplished by first selecting Calibrate pH from the Configure System menu This action brings up a new screen as shown below in Figure 3 6 Fig...

Page 36: ...o START remove the syringe from the priming valve wait until the pump stops then close the valve Please realize that this screen only gives instructions accessing this screen by itself will not prime the pumps 3 2 Reviewing and Editing Operating Parameters The CHDF 2000 is configured with default values for 14 operating parameters that control specific system functions Review all operating paramet...

Page 37: ...librate the conductivity sensor that is onboard the CHDF 2000 When this command is selected the display changes to Cell K Rotate the selector knob to the correct value then push the knob to enter the value The selected value should be in the range 0 8 to 2 0 Run Time This number sets the length of the X axis on the display when data is viewed during a CHDF run The default value is 15 minutes When ...

Page 38: ...ump Standby Sets how long the pump will run before it automatically shuts off Time is measured in units of minutes and a setting of 0 will cause the pump to run indefinitely The parameter is best used in conjunction with an autosampler For example it can be used to shut down the pump after the instrument has concluded an overnight run However the Pump Standby time can expire before all samples are...

Page 39: ...his parameter rotate the selector knob until the current value of the eluant temperature appears Then push the knob to save the changes Auto Zero Turns the autozeroing ability of the instrument on or off When on an autozero of the detector baseline takes place each time an injection occurs The autozero sets the detector baseline to the mAU value defined in AU Zero Offset Once selected this paramet...

Page 40: ...and number appears next to this Rotate the selector knob to the desired value then push the knob to save the settings A value of 5 is recommended Next Screen In this instance selecting Next screen takes you back to the first Configure System screen Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 41: ... Hudson St Northborough MA 01532 Ph 508 393 0155 Fax 508 393 5476 www matec com e mail colloid matec com Chapter 4 Operating the CHDF 2000 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 42: ...Prime the pump to remove air bubbles from the eluant supply tubes or to purge eluant from the system before you use new eluant To prime a pump 1 From the Home screen choose Prepare system The Prepare System screen appears as shown in Figure 4 1 Figure 4 1 The Prepare System Screen 2 Choose Prime pump The Prepare System Prime Pump screen appears Figure 4 2 Artisan Technology Group Quality Instrumen...

Page 43: ...ringe screen step 1 Attach the syringe to the priming valve Figure 4 3 Inserting Syringe in Pump Priming Valve 4 Open valve screen step 2 With the syringe attached to the priming valve open the valve by turning the top of the valve to the left see Figure 4 3 5 Draw eluant screen step 3 Carefully draw eluant into the syringe until Air bubbles disappear from the eluant inlet tubing to the pump The s...

Page 44: ...ording to the time set for the Priming Pump Time system parameter Refer to Chapter 3 for more information 4 2 Analyzing Samples This section includes procedures for the following tasks Performing standard sample analysis Using standby mode when you are not analyzing a sample 4 2 1 Performing Standard Manual Sample Analysis Sample can be injected manually as described here or using an autosampler T...

Page 45: ...ate the handle of the manual injector valve to the Inject position to inject the sample and start the selected method The Analyze Monitor Samples screen appears Figure 4 5 Remove the sample syringe 6 Rinse the injection valve with GR 500 eluant Repeat the above steps to inject the marker solution The recommended marker injection time is 60 seconds 7 During the fractionation the results will be dis...

Page 46: ...ure 4 6 The Standby Display Using automatic pump standby mode In automatic pump standby mode the pump or pump shut off after a user specified time interval at the end of a run Note The pump standby time must be either equal to or shorter than the lamp standby time You set the time interval by defining the value of the Pump Standby Delay operating parameter Refer to Chapter 3 Reviewing and Editing ...

Page 47: ...8 393 5476 www matec com e mail colloid matec com Exiting from Standby Mode To exit from Standby mode choose Home screen The lamp turns on the detector recalibrates and the Home screen reappears Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 48: ...on St Northborough MA 01532 Ph 508 393 0155 Fax 508 393 5476 www matec com e mail colloid matec com Chapter 5 Troubleshooting the CHDF 2000 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 49: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 50: ...e system pressure exceeds the high pressure limit for at least two seconds You configure the high pressure limit using the Pressure Limit Set operating parameter refer to Chapter 3 Reviewing and Editing Operating Parameters Figure 5 1 High Pressure Limit Exceeded Error Message Recommended response High pressure sometimes occurs if you are priming the pump with a cartridge installed To prevent high...

Page 51: ...tting 2 to Fitting 1 then connect the tube from Fitting 1 to Fitting 2 Disconnect Fitting 3 Connect the tube on Fitting 4 to Fitting 3 and Fitting 3 to Fitting 4 Run the pump for 15 minutes Reconnect all fittings as they are normally and try running the pump again If the high pressure message does not re appear your backflushing was successful 4 Snip off end of the glass capillary tube in the bott...

Page 52: ... knob to clear the Low Pressure Limit Exceeded error message 5 1 3 Power Lost The Power Lost error message Figure 5 3 appears if the CHDF 2000 temporarily loses power during sample analysis Figure 5 3 Power Lost Error Message Recommended response This message occurs when the CHDF 2000 temporarily loses power due to a power outage or inadvertent powering off of the system during sample analysis Bef...

Page 53: ...ion Press the selector knob to clear the Detector Calibration Failure error message 5 2 Quick Troubleshooting Checklists Use the tables in this section to quickly identify corrective actions for common CHDF problems This section covers the following topics General troubleshooting Fractionation troubleshooting 5 2 1 General Troubleshooting Use Table 5 1 to quickly identify corrective actions for ge...

Page 54: ... 5 2 to quickly identify corrective actions for fractionation system problems Table 5 2 Fractionation Problems Quick Troubleshooting Checklist Symptom Possible Cause Corrective Action Refer to Erratic retention times Air bubble in pump head Degas all eluants re prime pump Section 4 1 Malfunctioning pump check valves Clean or replace pump check valves Section 6 1 3 Leak at tube fitting Check that a...

Page 55: ...omposition Low column temperature Increase column temperature Increased sample retention times marker retention times correct Eluant conductivity too high Prepare new eluant check conductivity Section 2 8 No peaks detected Plugged capillary Change eluant backflush cartridge snip off end of capillary or replace top cartridge Sections 5 1 and 6 6 Pump shutdown Start pump Chapter 4 Reduced retention ...

Page 56: ...rease value of Lamp Standby Delay parameter Section 3 2 Incorrect wavelength for eluant Change eluant or wavelength UV absorbance specifications of eluant supplier Baseline drift slow Eluant contaminated Use fresh eluant Decreased UV lamp energy Check lamp energy Section 5 3 Ambient temperature fluctuations Prepare the system to proper equilibration UV detector flow cell leaking Check flow cell ti...

Page 57: ...es to Inject and the inject signal is displayed in volts When the selector knob is pressed a second time the display changes to Load and the load signal is displayed in volts Test Output Volts This voltage is used to run a strip chart recorder or other recording device It is not important when a computer is used to run the CHDF 2000 When selected the display changes to DAC Value and a voltage is d...

Page 58: ...following sections Test START IN Signal Diagnostic The Test START IN Signal diagnostic provides two functions Tests the START IN signal on the signal connector module Tests the manual injector valve Testing the START IN signal To test the START IN signal on the signal connector module 1 Place the manual injector valve in the Load position 2 Choose Test START IN Sig LOAD should appear in the displa...

Page 59: ...signals 6 Press the selector knob to return to the Run Diagnostics screen Test Keypad Diagnostic Use the Test Keypad diagnostic to test the keypad control keys To test the keypad control keys 1 Choose Test Keypad The display shows a line of characters each of which corresponds to one of the controls keys on the control panel keypad 2 Test the control keys by pressing each key and verifying that th...

Page 60: ...igured as a constant of the instrument optics at the time of the Autozero function K is held constant throughout a given separation while the values of REF and SMP are continuously monitored to compute the value of AU 2 Rotate the selector knob to vary L in 1 nm increments while you monitor the changes in REF SMP and AU values 3 Press the selector knob to return to the Run Diagnostics screen AU Ab...

Page 61: ...Hudson St Northborough MA 01532 Ph 508 393 0155 Fax 508 393 5476 www matec com e mail colloid matec com Chapter 6 Maintaining the CHDF 2000 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com ...

Page 62: ...eluant pump Figure 6 1 Pump Components This section includes the following pump maintenance procedures Replacing the plunger seals Cleaning and replacing the plunger Cleaning and replacing the check valves Replacing the in line filter Handling plugged cartridges Tee Connector Left Pump Head Assembly Eluant Inlet Tee Outlet check valve assembly Piston Indicator Rod Right pump head assembly Inlet Ch...

Page 63: ...n the priming valve 3 Place the eluant reservoir below the level of the eluant inlet tube Remove the inlet line from the inlet tee of the pump and allow the eluant to drain back into the reservoir 4 Set the Priming Flow Rate parameter to 0 5 mL min refer to Section 3 3 Reviewing and Editing Operating Parameters 5 Use the Prime Pump option to turn on the pump refer to Section 4 2 Priming the Pumps ...

Page 64: ...lease the piston indicator rod to have it rest on the piston inside the pump This prevents damage to the rod during maintenance 11 Slide the pump head assembly into position over the plunger and replace the two pump head mounting bolts Tighten the bolts alternately If you are replacing plunger seals in both pump heads repeat steps 4 through 11 for the other pump head 12 Use the Prime Pump function...

Page 65: ...Allen wrench Snap ring pliers Plunger insertion tool Fine abrasive such as toothpaste Accessing the Plunger To remove the plunger from the pump head 1 Remove the pump head as described in Section 6 1 1 Replacing the Plunger Seals 2 Remove the four plunger support screws with the 9 64 inch Allen wrench Carefully slide the head support assembly and the indicator rod off the pump and set the head sup...

Page 66: ...he seal Cleaning the pump plunger periodically can prolong the life of the seal Clean the plunger with a fine pumice or clear abrasive toothpaste Rinse the plunger thoroughly with water or methanol to remove all traces of the abrasive The parts of the plunger assembly are shown in Figure 6 6 Figure 6 6 Disassembled Plunger Inspecting the Plunger After you clean the plunger inspect it for damage by...

Page 67: ...e head support assembly with the indicator rod in the upper right corner Alternately tighten the four screws 5 Wet the seal and plunger with eluant 6 Gently slide the pump head onto the plunger and alternately tighten the two mounting bolts Check the head alignment visually by observing the gap between the pump head and the pump head support Make sure the indicator rod moves freely If the head is ...

Page 68: ...ine cleaning To clean the check valves while they are installed in the system 1 Set the following operating parameter values refer to Section 3 3 Reviewing and Editing Operating Parameters Priming Flow Rate to 10 0 mL min Priming Pump Time to 15 min 2 Fill the pump eluant reservoir with at least 400 mL of cleaning eluant 3 Open the priming valve of the pump to be cleaned 4 Position a large beaker ...

Page 69: ...ir and prime the pump see Section 4 2 Priming the Pumps Check for leaks Replacing an Outlet Check Valve Seal and Gasket Required materials 5 16 inch open end wrench 1 2 inch open end wrench Dentist s pick Thick plastic pen cap or other blunt tool such as the plunger insertion tool To replace the outlet check valve seal and gasket 1 Use the 5 16 inch wrench to disconnect the outlet tubing 2 Use the...

Page 70: ...he instrument power and disconnect the power cord before you perform this procedure Required materials Two 5 8 inch open end wrenches One 5 16 inch wrench New filter insert from start up kit To replace the in line filter 1 Reduce the pump flow rate to 0 mL min 2 Hold the in line filter on the front of Pump A with a 5 8 inch wrench while you disconnect the two compression screws with a 5 16 inch wr...

Page 71: ...ection includes the following injector maintenance procedures Handle screw Handle Knob Set screws 2 Needle guide Pressure adjusting screw Body Thrust bearing Spring washers 4 Rotor pin Position sensing switch Seal pins 4 Needle port tube Bearing ring Isolation seal Rotor seal Stator ring Stator locating pin Stator face assembly Stator locating hole Stator Stator screws 3 Needle seal 60 stop ring T...

Page 72: ... slots on the adjusting screw 5 Use the knob as a wrench to tighten the pressure adjusting screw approximately 1 20th of a turn Use the 20 dial markings on the body and the painted spot on the screw to gauge how far to tighten the screw 6 If the new setting fails to accomplish leak free operation repeat step 5 only once Avoid excessive tightening which increases rotor seal wear 7 Finish by retight...

Page 73: ... one half turn 2 Note the original position of the two red dots 3 Orient the rotor seal as shown in Figure 6 11 with the rotor seal slots facing the stator Figure 6 11 Rotor Seal Orientation Viewed from Stator 4 Replace the stator ring so that the pin in the 60 stop ring enters the mating hole in the stator ring 5 Install the stator face assembly on the stator The three pins on the stator face ass...

Page 74: ...e of the correct type and gauge to load the injector Checking the Needle Seal To check for a proper seal around the needle 1 Turn off the pump using the Prime Pumps option 2 Fill the syringe with water 3 Place the injector in the Load position and slowly discharge the water in the syringe into the injector 4 Note the low resistance to syringe discharge 5 Fill the syringe with water and repeat step...

Page 75: ...emove it 5 Using a wire with a small right angle bend formed in the end carefully remove the Teflon seal ring from the valve body Do not scratch the sealing surface around the seal 6 Pull the plastic tip from the stem using tweezers or small pliers 7 By hand insert a new plastic tip into the stem assembly blunt end first Seat the tip firmly with light pressure against a clean hard surface 8 Insert...

Page 76: ...om application to application If the lamp no longer provides an adequate signal to noise ratio for your application replace it It is not unusual for lamps to show a 30 to 50 percent decrease in intensity before the noise increases by a factor of two Lamp Timer The detector lamp has a life of approximately 1000 hours One of the leads attached to the lamp includes a 1000 hour timer to indicate lamp ...

Page 77: ... cover the UV lamp automatically turns off Before removing the lamp set the wavelength to 230 nm If the lamp is bad or fails to light and this wavelength setting is not possible remove the lamp and replace it as follows Caution The lamp housing becomes extremely hot during operation To prevent burns to the skin allow the lamp to cool for one hour before removing 1 Turn off the unit power and allow...

Page 78: ... see Figure 6 14 5 Pull the lamp assembly out of the lamp housing Figure 6 15 Figure 6 15 Removing the Lamp 6 From the top of the lamp assembly remove the center adjustment screw along with the star washer and the hex nut Place the lamp assembly on a flat surface with the bulb positioned upward Figure 6 16 Lamp connector Center adjustment screw Allen head screw Lamp timer Artisan Technology Group ...

Page 79: ...stalling the New Lamp Unpack the lamp from its packing material The new lamp may vary slightly from the lamp illustrated in Figure 6 16 Note Do not touch the glass bulb on the new lamp Dirt or fingerprints on the bulb affect detector operation If the lamp needs cleaning gently clean the bulb with ethanol and lens tissue Do not use any abrasive tissue or excessive pressure To install the new lamp 1...

Page 80: ...r adjustment screw with the hex nut and star washer in the center opening of the adjustment plate In preparation for maximizing the lamp energy turn the screw until the lamp assembly moves about 3 4 mm away from the adjustment plate 5 Place the lamp assembly onto the lamp housing Make sure the lamp leads slide freely into the housing slot 6 Secure the lamp assembly to the housing and tighten the t...

Page 81: ...the result pass fail of the Calibrate UV power on diagnostic 3 Choose Run diagnostics The Run Diagnostics screen appears see Figure 5 5 4 Choose Show Energy AU λ λ λ λ The current wavelength λ setting the detected absorbance AU and the current lamp sample SMP and reference energy REF values appear see Figure 5 6 If the result of the Calibrate UV power on diagnostic was Pass go to step 10 If the re...

Page 82: ...art this procedure from step 1 10 Rotate the selector knob to set the wavelength AU to 656 nm Caution To prevent burn injury do not touch the lamp assembly directly 11 Observe the displayed REF value while you turn the center adjustment screw see Figure 6 14 to maximize the REF value Be sure the displayed value is stable before continuing 12 If the screws on top of the lamp assembly see Figure 6 1...

Page 83: ...ics use a non metal screwdriver to adjust the Brightness and Contrast controls 3 Observe the control panel display while you use a non metal flat blade screwdriver to adjust the Brightness adjustment screw on the back of the front door Error Reference source not found Figure 6 19 Brightness and Contrast Adjusting Screws 5 Observe the control panel display while you use a non metal flat blade screw...

Page 84: ...the cartridge with solvent or backflush it to remove a plug If either of these techniques are not successful there are two techniques discussed here that will ultimately take care of the problem The first snipping off the end of the capillary assumes the plug is in the very end of the cartridge and that this section will be removed when snipped off The second method described here changing the fra...

Page 85: ...ed Re connect the four fittings Do not over tighten the fittings Tighten turn past finger tight 10 Run known samples or standards to see if the cartridge is working properly 6 6 2 Replacing the Fractionation Cartridge If the snipping procedure described in the above section does not solve your problem the fractionation cartridge on the CHDF 2000 will need to be replaced Once a new cartridge is pur...

Page 86: ...8 at this point will result in fractionation capillary breakage Unscrew the three screws holding the cartridge in place 4 Gently remove the cartridge from the instrument 5 Remove the plastic caps from the tube ends of the new cartridge if they are present Take the new cartridge and place it gently into the instrument 6 Replace the cartridge using the 3 screws provided 7 Reconnect the cartridge tub...

Page 87: ...l metal piece screwed into a larger metal piece The smaller metal piece contains the filter element Unscrew the smaller piece and remove it from the larger piece 4 Remove the fritted stainless steel filter element from the piece you just unscrewed 5 If you analyze polymer samples unplug the filter element by sonicating the element in acetone or tetrahydrofuran for at least 15 minutes If you analyz...

Page 88: ...l service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE INSPECTION Remotely inspect equipment before purchasing with our interactive website at www instraview com LOOKING FOR MORE INFORMATION Visit us on the web at www artisantg com for more information on ...

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