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When each of these commands is selected, the display changes so that a number may be
entered. The parameters are described and set as follows:
Set Flow Rate - Sets the flow rate at which eluant is pumped through the fractionation
cartridge in units of ml/min. When selected, the display changes to “Rate = “. Turn the
selector knob to the desired value, then push the knob to enter the value. A typical flow rate is
1.4 ml/min. This is the same parameter as was set in the Configure System screen seen in
Figure 3.5.
Set Wavelength - This command sets the wavelength at which the UV/VIS detector will work.
The wavelength is in units of nanometers. To change the wavelength, select Set Wavelength.
The display changes to “Run Wavelength = “. Rotate the selector knob left and right until the
desired wavelength appears. Push the selector knob to enter the value. A typical wavelength is
220 nm.
Set Cell K - This parameter is used to calibrate the conductivity sensor that is onboard the
CHDF 2000. When this command is selected, the display changes to “Cell K:”. Rotate the
selector knob to the correct value, then push the knob to enter the value. The selected value
should be in the range 0.8 to 2.0.
Run Time - This number sets the length of the X axis on the display when data is viewed
during a CHDF run. The default value is 15 minutes. When this command is selected the
display changes to “Run Time:”. Rotate the selector knob to the desired value, then push the
knob to enter the value.
Heater - This parameter is used to set the temperature of the fractionation cartridge. The
default is 35
°
C. When this parameter is selected the display changes to “Cartridge Temp: “.
Rotate the selector knob to the desired value, then push the knob to enter the value.
NOTE: Changing the cartridge temperature requires the measurement of a new
calibration curve.
Next Screen - Displays the second Configure System screen, seen in Figure 3.9 below.
The second Configure System screen is displayed by selecting Next Screen in the first Configure
system screen. The second Configure System screen is shown below in Figure 3.9.
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