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Home Screen to get back to the top menu. Here is how to set the four parameters listed in the
Configure System screen seen in Figure 3.5.
Flow Rate - This sets the rate at which eluant is pumped through the fractionation cartridge. To
change the flow rate, simply select it, then rotate the selector knob left or right until the flow rate
you desire appears on the screen. Push the selector knob to enter the value. Normally, a value of
1.4 should be selected.
Set Wavelength - This command sets the wavelength at which the UV/VIS detector will work.
The wavelength is in units of nanometers. To change the wavelength, select Set Wavelength,
rotate the selector knob left and right until the desired wavelength appears. Push the selector
knob to enter the value. The recommended value is 220 nm.
Calibrate pH - Select this option when you need to calibrate the pH sensor on the CHDF 2000.
You will need three buffer solutions, typically of pH 4.0, 7.0, and 10.0 to calibrate the pH sensor.
The sensor is calibrated by alternately immersing the sensor in the three buffers, then entering the
pH value of the buffer into the instrument. This is accomplished by first selecting Calibrate pH
from the Configure System menu. This action brings up a new screen as shown below in Figure
3.6.
Figure 3.6 The Calibrate pH screen
As stated in the screen, first highlight the pH that is going to be set by turning the selector knob.
Click the knob, then turn it to select the pH of the buffer to be used. Place the pH sensor in the
buffer, wait for the Prove mV reading to stabilize, then turn the selector knob to the right to
highlight either Accept Acid, Accept neut, or Accept base. Press the selector knob to enter the
pH value. Three buffers of different pH must be used to calibrate the sensor.
Prime Pumps - The pumps are primed by drawing eluant into the pump heads to get rid of air
bubbles. The process is described in a screen that can be accessed by selecting Prime Pumps.
The following screen appears.
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