LI-COR LI-600, Instruction Manual

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LI-600 Porometer/Fluorometer Instruction Manual
3-2
Techniques
The LI-600 can measure the top or the bottom of a leaf.
The following techniques help ensure the quality and
accuracy of your measurements.
l
Maintain the original orientation of the leaf. Do
not alter the angle or positioning of the leaf you are
measuring.
l
Maintain the light conditions of the leaf. Do not
alter the light on or shade the leaf you are meas-
uring. If, while taking a measurement, you shade the
leaf you plan to measure next, unshade the leaf and
wait 60 seconds before measuring it.
l
Match the quantum sensor's light conditions to
those of the leaf.
If the leaf you measure is in the
light, ensure that the quantum sensor is also in the
light. If the leaf you measure is in shade, ensure that
the quantum sensor is also in the shade.
l
Avoid measuring a leaf with water on it. If the
leaf you plan to measure has water or condensation
on it, gently and thoroughly dry the top and bottom
of the leaf.
l
Avoid clamping onto the veins of the leaf. The
uneven surface may prevent the aperture clamp from
properly sealing. If in auto-mode, the instrument
may not take the measurement. If in manual mode,
the logged measurement may be inaccurate.
l
Fully fill the aperture with the leaf. To get reli-
able stomatal conductance readings, the chamber
must be completely covered and sealed. When using
an LI-600 equipped with a fluorometer, fluorescence
readings can be taken without filling the aperture.