LI-COR LI-600, Instruction Manual

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LI-600 Porometer/Fluorometer Instruction Manual
9-16
Type Pre-flash F Flash F
Calculated
Yield
Dark-adapted Fo Fm Fv/Fm
Light-adapted Fs Fm’ Φ PSII
Table 9-1 . Dark and light adapted measurement para-
meters.
Steady-state fluorescence
Steady-state fluorescence logged is the final value
received prior to the onset of the flash. A zero offset is
applied to the de-modulated fluorescence that removes
small artifacts that arise from imperfect discrimination
or electronic noise. The offset is stored as a calibration
constant and can be re-set by the user. Steady-state fluor-
escence removes the zero offset following.
9-46
where v
F
is read from the ADC and Z
flr
is a factory coef-
ficient. The measurement frequency is set in the con-
figuration.
Rectangular flash
A rectangular flash delivers a user-selectable flash intens-
ity (µmol m
-2
s
-1
) for a user-selectable amount of time
(ms). The LI-600 converts that to a frequency during the
flash using the factory calibration value.
Multiphase flash
The LI-600 can perform multiphase flashes for con-
ditions where it is difficult to fully saturate with rect-
angular flashes (Loriaux et al, 2013). A multiphase flash
delivers a flash in three phases and requires inputs of the
length of each phase (P1_dur, P2_dur, P3_dur) in msec,