Page 4 Operating Manual Leica EM FC7 01/10
Table of contents
1.
Safety Introduction
................................................................................................................................... 6
1.1
Symbols and abbreviations .................................................................................................. 10
1.2
Abbreviations ....................................................................................................................... 10
2.
Identification
............................................................................................................................................ 11
2.1
Product ................................................................................................................................. 11
2.2
Manufactured by .................................................................................................................. 11
3.
Introduction
.............................................................................................................................................. 12
3.1
Overview .............................................................................................................................. 12
3.2
Instrument Function ............................................................................................................. 13
3.3
Advanced touch screen control unit..................................................................................... 14
3.4
Basic touch screen control unit ............................................................................................ 15
4.
Installation and Setup
............................................................................................................................ 16
4.1
Packing list ........................................................................................................................... 16
4.2
Preparation of the ultramicrotome UC7 or UC6 ................................................................... 18
4.3
Unpacking the cryochamber EM FC7 .................................................................................. 19
4.4
Mounting the EM FC7 onto the Ultracut UC7 ...................................................................... 22
4.5
Connecting the Cryochamber .............................................................................................. 24
4.6
Connecting the LN
2
hose of the pump ................................................................................. 25
4.7
Connecting the antistatic device EM CRION with discharge mode ..................................... 26
4.8
Connecting the EM CRION with discharge and charge mode............................................. 26
4.9
Installing the Micromanipulator ............................................................................................ 28
4.10
Repacking to prevent damage during transportation........................................................... 29
4.11
Storage location for the instructions..................................................................................... 29
5.
Operation
.................................................................................................................................................. 30
5.1
Inserting the cryo specimen holder ...................................................................................... 30
5.2
Inserting knives into the knife holder.................................................................................... 30
5.3
Inserting the knife holder...................................................................................................... 31
5.4
Inserting the specimen ......................................................................................................... 32
5.5
Using the antistatic device EM Crion with discharge mode ................................................. 33
5.6
Using the EM Crion with discharge and charge mode......................................................... 34
5.7
Advanced touch screen control unit to operate the EM FC7 ............................................... 35
5.8
Basic touch screen control unit to operate the EM FC7 ...................................................... 39
5.9
Operating the micromanipulator........................................................................................... 41
5.10
Set of Cryotools.................................................................................................................... 46
5.10.1
Unpacking.................................................................................................................... 46
5.10.2
Device function and operation..................................................................................... 46
5.11
Refilling the Dewar ............................................................................................................... 51
5.12
Finishing work with the EM FC7 .......................................................................................... 52
5.13
Maintenance of the valves ................................................................................................... 52
6.
Technical specifications
....................................................................................................................... 53
Summary of Contents for EM FC7
Page 1: ...Leica EM FC7 Operating Manual Version 162220032 01 2010...
Page 6: ...Page 5 Operating Manual Leica EM FC7 01 10...
Page 21: ...Page 20 Operating Manual Leica EM FC7 01 10 Remove LN2 hose and take away foam packaging...
Page 38: ...Page 37 Operating Manual Leica EM FC7 01 10 MENU User or Sample profile 1 2...
