Page 46 Operating Manual Leica EM FC7 01/10
5.10
Set of Cryotools
The Set of cryotools is used for handling frozen hydrated sections, Tokuyasu
sections as well as collecting polymer sections.
5.10.1 Unpacking
The set consists of 4 parts: the base (with a flip-over bridge for preloading /
releasing of grids), the spring loaded grid holder, the section press and the transfer
container.
5.10.2 Device function and operation
The base of the cryotools is mounted on the rotating knife holder with a locking
screw.
A pre cooled grid is clamped in the spring loaded grid holder (2). The grid holder is
shifted forward until the grid is close to the knife edge. Cryosectioning is carried out.
Once the ribbon of frozen hydrated sections is on the grid, the holder with the grid is
pulled back in south direction. The grid is then placed onto the flip -over bridge (3)
and sections are pressed with the section press (4). After flattening the sections on
the grid, the grid is transferred to a transfer container (5).
2
4
3
5
1
Summary of Contents for EM FC7
Page 1: ...Leica EM FC7 Operating Manual Version 162220032 01 2010...
Page 6: ...Page 5 Operating Manual Leica EM FC7 01 10...
Page 21: ...Page 20 Operating Manual Leica EM FC7 01 10 Remove LN2 hose and take away foam packaging...
Page 38: ...Page 37 Operating Manual Leica EM FC7 01 10 MENU User or Sample profile 1 2...
