Page 38 Operating Manual Leica EM FC7 01/10
1: Temperature Unit. After pushing the menu button on the main screen the
temperature unit can be selected in the Edit User menu.
2: FC Mode. The FC7 chamber is equipped to toggle between three different
modes. Push the menu button on the main screen and select the Edit or Add New
sample menu.
x
Standard mode is preferable used for regular sectioning e.g. Tokuyasu or polymer
sectioning at dry conditions.
x
High Gas Flow is preferable used to increase GN
2
gas flow below a temperature of -
140°C in order to reduce ice contaminations.
x
Wet Sectioning mode is used for polymer sectioning at wet conditions using DMSO
or similar liquids in order to set the temperature of knife at a maximum of -40°C and
the temperature of the sample minimum of -170°C. To achieve the minimum
temperature of the sample the specimen arm must be moved to its lowest position
during the cooling process. Thus, parts of the cooling element of the sample holder
are placed into LN
2
which facilitates cooling down the sample at a minimum of -
170°. The temperature will be kept during the sectioning process.
Summary of Contents for EM FC7
Page 1: ...Leica EM FC7 Operating Manual Version 162220032 01 2010...
Page 6: ...Page 5 Operating Manual Leica EM FC7 01 10...
Page 21: ...Page 20 Operating Manual Leica EM FC7 01 10 Remove LN2 hose and take away foam packaging...
Page 38: ...Page 37 Operating Manual Leica EM FC7 01 10 MENU User or Sample profile 1 2...
