Page 41 Operating Manual Leica EM FC7 01/10
5.9 Operating
the
micromanipulator
The micromanipulator in conjunction with the EM CRION with discharge and charge
mode is indented to use for section collection of frozen hydrated sections as well as
Tokuyasu sections. The micromanipulator allows the grid to be exactly positioned
close to the knife edge using the micrometer gauges. Once these positions are
defined, fast retraction of the grid can be performed manually prior to sectioning to
prevent possible influence of the grid on the ionizer. The Leica EM CRION is used
in discharge mode in order to reduce electrostatic charging while sectioning.
When it is time to place the section ribbon on the grid, the user can quickly return to
the pre-set grid position. The ribbon is then attached to the grid using the charge
mode of the LEICA EM CRION, which is operated by a footswitch. Thus, the
specimen adheres to the grid without the need of a section press.
As a grid box can be placed near the knife, the micromanipulator can be used to
easily place the grid into its storage position.
Place the grid-box holder with its magnet
onto the knife holder.
Insert the grid box in the holder.
Summary of Contents for EM FC7
Page 1: ...Leica EM FC7 Operating Manual Version 162220032 01 2010...
Page 6: ...Page 5 Operating Manual Leica EM FC7 01 10...
Page 21: ...Page 20 Operating Manual Leica EM FC7 01 10 Remove LN2 hose and take away foam packaging...
Page 38: ...Page 37 Operating Manual Leica EM FC7 01 10 MENU User or Sample profile 1 2...
