Leica EM FC7, Operating Manual

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Page 12 Operating Manual Leica EM FC7 01/10
3. Introduction
3.1 Overview
The Leica EM FC7 is the low temperature sectioning system for the Leica Ultracut
UC7 (and UC6) ultramicrotome. It is used for semi- and ultrathin cryosectioning of
biological and industrial samples. This includes Frozen Hydrated Sectioning (FHS)
of vitrified specimens, cryoprotected biological samples (Tokuyasu technique) and
soft industrial specimens such as rubbers and polymers.
The Leica EM FC7 has been specifically designed for maximum ergonomics and
ease of use. It has an integrated chamber illumination, allows to use an integrated
antistatic device and provides a wide temperature range from –15 °C to – 185 °C for
cryosectioning. All controls are via the control unit (CU) of the ultramicrotome to
save workspace on the table and provide maximum comfort and ergonomics for the
operator.
The EM FC7 system consists of
· Cryochamber with integrated LED illumination
· Dewar vessel on mobile trolley
· Liquid nitrogen pump
· Power supply
· Connecting cables, LN
2
hose
· Accessories box containing 2 rotating knife holders, 1 standard specimen
holder, specimen carriers, special keys, tools and forceps
The „complete working outfit“contains in addition to the items mentioned above:
· Flat specimen holder
· CRION antistatic device, remote controlled
This allows controlling the ioniser from either the “Advanced touch screen
controller“or the “7” touch screen controller“of the Ultracut UC7.
Recommended accessories are
· Micromanipulator
· Filling System for LN
2
Dewar