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Page 9 Operating Manual Leica EM FC7 01/10

HAZARD WARNING

LIQUID NITROGEN, LN

Suffocation

Any vessel containing LN

is a potential hazard

One litre LN

produces 700 litres N

gas

- N

gas is odourless and tasteless

Oxygen levels can quickly drop in confined spaces due to displacement of

oxygen

- by

when using or dispensing large volumes of LN

This can cause immediate fainting and unconsciousness

Always use LN

in well-ventilated areas

Treat it with respect!

Storage

For reasons mentioned above do not store full LN

Dewars in confined

spaces

Burns

- LN

boils at -196°C. It is extremely cold and can cause serious burns. Please

read the safety instructions provided with all Leica products for the correct

handling of liquid nitrogen!

Summary of Contents for EM FC7

Page 1: ...Leica EM FC7 Operating Manual Version 162220032 01 2010...

Page 2: ...ring techniques used to provide our customers with excellent products Any copyrights of this document are retained by Leica Mikrosysteme GmbH Vienna Any reproduction of text and illustrations or any p...

Page 3: ...nual Leica EM FC7 Serial Number Date of purchase For the instrument serial number please refer to the name type label on the back of the instrument Please read this instruction manual carefully before...

Page 4: ...ed for future reference Texts schedules and tables may not be copied reproduced or divulged to third parties without our express consent It should be noted that this Technical Documentation does not c...

Page 5: ...4 8 Connecting the EM CRION with discharge and charge mode 26 4 9 Installing the Micromanipulator 28 4 10 Repacking to prevent damage during transportation 29 4 11 Storage location for the instructio...

Page 6: ...Page 5 Operating Manual Leica EM FC7 01 10...

Page 7: ...ays in a low oxygen environment then death may occur If breathing stops apply artificial respiration at once and notify doctor and ambulance immediately For the reasons given above never put LN2 Dewar...

Page 8: ...asses b with side protection which are open at the top and at the bottom Only use boots if you have loose not narrow trousers coming outside the boots d and completely covering the gap Wear only open...

Page 9: ...iling point 183 C When the exchange surfaces are extensive enough oxygen from the air will be taken up in exchange for nitrogen LN2 with high liquid oxygen content has a faintly bluish colour Concentr...

Page 10: ...to displacement of oxygen by N2 when using or dispensing large volumes of LN2 This can cause immediate fainting and unconsciousness Always use LN2 in well ventilated areas Treat it with respect Stora...

Page 11: ...stem if it is ignored Note This symbol indicates further information relating to a previous explanation which does not have a safety critical function However it is important to observe this informati...

Page 12: ...EM FC7 01 10 2 Identification 2 1 Product Leica EM FC7 Cryo chamber 2 2 Manufactured by Leica Mikrosysteme GmbH Hernalser Hauptstrasse 219 A 1170 Vienna Tel 43 1 488 99 0 Fax 43 1 488 99 350 Internet...

Page 13: ...re range from 15 C to 185 C for cryosectioning All controls are via the control unit CU of the ultramicrotome to save workspace on the table and provide maximum comfort and ergonomics for the operator...

Page 14: ...chanical stability After connection of the LN2 hose to the LN2 filled Dewar via the automatic pumping system the pump ensures a stable low temperature environment around the knife specimen area by pum...

Page 15: ...Leica EM FC7 01 10 3 3 Advanced touch screen control unit After connecting and switching on the EM FC7 and the EM CRION to Advanced touch screen control unit of the Ultramicrotome UC7 the touch scree...

Page 16: ...ca EM FC7 01 10 3 4 Basic touch screen control unit After connecting and switching on the EM FC7 and the EM CRION to the 7 touch sensitive control unit of the Ultracut UC7 the main screen of the contr...

Page 17: ...er The case for accessories contains Specimen carriers 50 pcs with concentric rings Specimen carriers 50 pcs with slot Allen key 3 mm Cryotool with M4 thread Collet specimen clamp Rotating knife holde...

Page 18: ...ng Manual Leica EM FC7 01 10 Optionally available accessories Micromanipulator for frozen hydrated sectioning Set of cryotools Dewar Refilling System Cryo Mesacut Mirror Trimming Tool Different Flat S...

Page 19: ...ter removal of armrest knife support and segment arc Activate the FC Mount Function of the Ultracut by pushing the FC Mount button of the controller With the FC Mount Function of the Ultracut UC6 or U...

Page 20: ...Page 19 Operating Manual Leica EM FC7 01 10 4 3 Unpacking the cryochamber EM FC7 Open box and lift out EM FC7 cryochamber and LN2 hose...

Page 21: ...Page 20 Operating Manual Leica EM FC7 01 10 Remove LN2 hose and take away foam packaging...

Page 22: ...Before mounting the EM FC7 on the Ultracut remove the transport locking screws on rear side and bottom knife plate of the cryochamber Keep the transport locking screws in the case for accessories for...

Page 23: ...of the cryochamber should be on the right side of the line Slide on the chassis of the Ultracut UC7 until the second indentation line Turn the hand wheel of the Ultracut UC7 to move the specimen arm...

Page 24: ...irmly connected by gently pulling in the south direction To increase the clamping remove lever a by unscrewing slot screw Reposition the lever an eighth counter clockwise on the clamping shaft and loc...

Page 25: ...instrument is connected to it according to the local regulations Unplug the instrument before installing or changing fuses In case a Leica EM UC7 instrument table is available place the power supply...

Page 26: ...rting the connection open the safety shutter a The clean Dewar vessel has to be filled with LN2 according to the safety precautions Remove the yellow protective cup from the lower end of the pump Slow...

Page 27: ...the electrode connect cable to earth in the case the PE ground through the mains cord is not properly functioning remote control cable connect the other end to the rear side of the controller power c...

Page 28: ...hing on off with the button on the screen of the controller it can be switched on off if a footswitch is connected on the controller and Bind Motor to EM Crion is enabled see EM UC7 manual As soon the...

Page 29: ...he cryochamber by unscrewing the screws and exchange it with the supporting plate of the micromanipulator supporting plate pair of forceps end stop grid box holder and two grid boxes Allen key screw i...

Page 30: ...f it is not the system may be damaged If the system needs to be moved again disassemble and pack it away by following the instructions listed in Unpacking the cryochamber 4 3 in the reverse order 4 11...

Page 31: ...ition initially subsequent reading of the next position turned 90 for trimming will be easy 5 2 Inserting knives into the knife holder Set the desired clearance angle typically 6 Insert the desired kn...

Page 32: ...d arrow that corresponds to the hole in the knife holder red arrow Insert the rotating knife holder containing knives into the chamber The rotating knife holder is now in the loading position It can o...

Page 33: ...cate knife edge Lock the specimen pin in the cryo specimen holder Turn the small black lever in front clockwise while holding the cryo specimen holder in position with the small black lever in the bac...

Page 34: ...ks If the sections tend to stick on the knife edge increase the intensity step by step until the sections begin to float in a nice ribbon over the knife edge If the sections begin to lift up decrease...

Page 35: ...e edge increase the intensity step by step until the sections begin to float in a nice ribbon over the knife edge If the sections begin to lift up decrease the intensity step by step Press the dischar...

Page 36: ...Page 35 Operating Manual Leica EM FC7 01 10 5 7 Advanced touch screen control unit to operate the EM FC7 1 2 3 4 5 6 9 8...

Page 37: ...Touch to activate storing mode followed by one of the four memory fields 5 The set temperatures of specimen knife and gas are now stored in the desired memory field 5 Memory fields Touch to activate...

Page 38: ...Page 37 Operating Manual Leica EM FC7 01 10 MENU User or Sample profile 1 2...

Page 39: ...low is preferable used to increase GN2 gas flow below a temperature of 140 C in order to reduce ice contaminations x Wet Sectioning mode is used for polymer sectioning at wet conditions using DMSO or...

Page 40: ...Page 39 Operating Manual Leica EM FC7 01 10 5 8 Basic touch screen control unit to operate the EM FC7 Menu 7 8 6 1 2 3 4 5...

Page 41: ...y buttons Press to activate the stored value The reading for the actual temperature shows the set value for approx 4 seconds To store a new value set temperature 3 and press one of the memory buttons...

Page 42: ...prevent possible influence of the grid on the ionizer The Leica EM CRION is used in discharge mode in order to reduce electrostatic charging while sectioning When it is time to place the section ribbo...

Page 43: ...its holder of the micromanipulator and slide it towards the end stop position With the micrometer gauges set the grid slightly below the knife edge with the z movement E W position of the sample and...

Page 44: ...y e g at the end of the Z movement assembly Avoid turning the micrometer gauges once the forceps is retracted The preset position would be changed thus the knife edge can be damaged if repositioning o...

Page 45: ...W position of the grid and repeat as above described If you want to place the grid in the grid box move the grid box holder aside with tweezers and retract the forceps holding the grid Turn the force...

Page 46: ...release the grid To use a new grid take out the forceps and warm it up as long as the forceps is dry To speed up the process a second forceps is recommended Or another possibility is to load the seco...

Page 47: ...on and operation The base of the cryotools is mounted on the rotating knife holder with a locking screw A pre cooled grid is clamped in the spring loaded grid holder 2 The grid holder is shifted forwa...

Page 48: ...nual Leica EM FC7 01 10 Insert cryo knife into knife holder Mount base of cryotools on rotating knife holder and lock Insert grid holder and transfer container into base of cryotools Flip section pres...

Page 49: ...ll black lever or a pair of forceps Move gird holder towards the grid and clamp it by releasing the clamping jaw Pull back grid holder towards you and flip bridge to the right using a pair of forceps...

Page 50: ...he left Push forward grid holder until the grid is placed over the bridge Close section press by using a pre cooled pair of forceps Flatten sections under the press by pushing on the small indentation...

Page 51: ...on the small indentation at the rear end with a small black lever or a pair of forceps and retract grid holder Transfer flattened grid into the transfer container The transfer container can be remove...

Page 52: ...of water and humid air into the valves Use the yellow protective cap immediately to close the lower end of the pump Before inserting the pump again dry pump and take off yellow protective cap For ease...

Page 53: ...ntistatic electrode x take out diamond knives x unlock torque limited screw holding the cryo specimen holder This will prevent mechanical damage of specimen holder and locking screw due to heat expans...

Page 54: ...ng speed 0 05 100 mm s wheel controlled Section thickness 0 15000nm wheel controlled Return speeds 10 30 50mm s Coarse knife movements N S 10 mm stepping motor Step control 0 1 15 m steps E W movement...

Page 55: ...CA EM FC7 mod le LEICA EM FC7 Type Typenbezeichnung type EM FC7 706030 656002 to which this declaration relates is in conformity with the following standards auf das sich diese Erkl rung bezieht mit d...

Page 56: ...auptstrasse 219 1170 Vienna Austria Tel 43 1 48899 Fax 43 1 48899350 LEICA and the Leica logos are registered trademarks of Leica IR GmbH Order no of the edition Operating Manual Leica EM FC7 Version...

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