Leica EM FC7, Operating Manual

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Page 13 Operating Manual Leica EM FC7 01/10
Leica EM FC7 mounted on Ultracut UC7 with Advanced touch screen control unit
3.2 Instrument Function
Operation of the EM FC7 is performed via the control Unit of the Ultracut UC7.
Before quickly cooling down for trimming and sectioning, the cryochamber has to be
unpacked, mounted on the EM UC7 and connected to the Ultracut UC7 CU. As the
cryochamber is mounted on the chassis of the ultramicrotome it is isolated from
external vibrations. The contact free through-the-wall specimen arm provides
optimum temperature and mechanical stability.
After connection of the LN
2
hose to the LN
2
filled Dewar via the automatic pumping
system, the pump ensures a stable low temperature environment around the knife-
specimen area by pumping LN
2
into a tank in the EM FC7 wall. This LN
2
is then
heated and the evaporating gas is used to cool down chamber, specimen and knife.
Temperature stability of specimen, knife and gas in the chamber are individually
controlled by regulators close to knife and specimen. The desired temperature can
be set, changed and stored by the operator via the control unit while the actual
temperatures are displayed on the CU.
After finishing cryosectioning, a bake-out function allows the cryochamber to heat
up to +110 °C before returning to room temperature (RT) automatically.