Page 34 Operating Manual Leica EM FC7 01/10
5.6
Using the EM Crion with discharge and charge mode
EM Crion with discharge and charge mode is operated by footswitches (except
setting the intensity).
For trimming:
For perfect ribbons of sections a perfect trimmed sample is mandatory.
It is recommended to use the discharge mode for trimming, because it eliminates
the sticking of the chips on the specimen and on the blade.
Set the intensity for trimming at the full voltage = all 10 indication marks are
illuminated.
Press the discharge footswitch to enable the antistatic performance or the electrode.
For sectioning:
Set the intensity to 5 indication marks. If the sections tend to stick on the knife edge,
increase the intensity step by step until the sections begin to float in a nice ribbon
over the knife edge. If the sections begin to lift up, decrease the intensity step by
step.
Press the discharge footswitch to enable the antistatic performance or the electrode.
For placing the section ribbon on the grid:
The ribbon is attached to the grid by pressing the charge footswitch. Thus, the
specimen adheres to the grid without the need of a section press.
The electrode will lose its power if its metal tip becomes covered with ice or
debris. If this occurs, just clean the ionizer tip with a fine brush with the
device switched off.
The electrode tip carries high voltage and must not be touched or allowed to
touch any parts of the cryo ultramicrotome while operation.
Switch off the antistatic device prior to any manipulation in the chamber.
Never heat the chamber with the electrode inside.
Summary of Contents for EM FC7
Page 1: ...Leica EM FC7 Operating Manual Version 162220032 01 2010...
Page 6: ...Page 5 Operating Manual Leica EM FC7 01 10...
Page 21: ...Page 20 Operating Manual Leica EM FC7 01 10 Remove LN2 hose and take away foam packaging...
Page 38: ...Page 37 Operating Manual Leica EM FC7 01 10 MENU User or Sample profile 1 2...
