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Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019

Introduction ...................................................................................................................... 7

Intended Use

...................................................................................................................................7

Image Viewing Restrictions for Primary Diagnosis

....................................................................................7

Supported Image Types

......................................................................................................................7

Aperio AT2 DX Viewing Stations and Monitors

........................................................................................8

Aperio AT2 DX System Information

.......................................................................................................8

Aperio ImageScope DX Overview and Quick Reference .............................................. 9

Open a Case in Aperio ImageScope DX

..................................................................................................9

Aperio ImageScope DX Window Overview

.............................................................................................9

Aperio ImageScope DX Quick Reference

..............................................................................................10

The Slide Tray

................................................................................................................................13

Work with eSlides in the Slide Tray

.................................................................................................13

View Different Areas of the eSlide

.....................................................................................................15

Change the Viewing Zoom Level

........................................................................................................16

View Detailed Data for an eSlide

.......................................................................................................16

Image Rendering

............................................................................................................................16

Keyboard Shortcuts

.........................................................................................................................17

Close Aperio ImageScope DX

............................................................................................................18

Basic eSlide Viewing ..................................................................................................... 19

Before You Begin

............................................................................................................................19

Open Case eSlides in Aperio ImageScope DX

........................................................................................19

Close a Case in Aperio ImageScope DX

...............................................................................................20

eSlide Quality Control

......................................................................................................................20

Basic eSlide Viewing

.......................................................................................................................20

View an eSlide

...........................................................................................................................21

View and Annotate eSlides............................................................................................ 23

Annotate eSlides

............................................................................................................................23

Draw Annotations

.......................................................................................................................23

Delete an Annotation

...................................................................................................................25

View eSlides With Annotations

..................................................................................................25

Contents

Summary of Contents for Aperio ImageScope DX

Page 1: ...Aperio ImageScope DX User s Guide MAN 0352 Revision A 20 May 2019...

Page 2: ...ution Federal U S law restricts this device to sale by or on the order of an appropriately licensed healthcare practitioner This manual is not a substitute for the detailed operator training provided...

Page 3: ...ghai PRC PC 200025 CHINA Tel 86 4008208932 Fax 86 21 6384 1389 Email service cn leica microsystems com Remote Care email tac cn leica microsystems com Danmark Tel 0080052700527 toll free In country Te...

Page 4: ...LLC Pinsky lane 3 letter A Saint Petersburg 197101 THE RUSSIAN FEDERATION Tel 8 800 555 49 40 toll free In country Tel 7 812 320 49 49 Email main bioline ru Sweden Tel 0080052700527 toll free In count...

Page 5: ...o ImageScope DX Quick Reference 10 The Slide Tray 13 Work with eSlides in the Slide Tray 13 View Different Areas of the eSlide 15 Change the Viewing Zoom Level 16 View Detailed Data for an eSlide 16 I...

Page 6: ...29 Track Pan and Zoom Navigation With the Track Map 29 Clear the Track Map 29 Open Multiple eSlides 30 Synchronize Navigation of Multiple eSlides 31 Viewing a Z Stack eSlide 31 Viewing and Navigating...

Page 7: ...pe DX review application and display The Aperio AT2 DX System is for creation and viewing of digital images of scanned glass slides that would otherwise be appropriate for manual visualization by conv...

Page 8: ...alibrated monitors that you receive as part of the Aperio AT2 DX System are supported for primary diagnosis As part of monthly maintenance we recommend that you do a visual quality check of your monit...

Page 9: ...ImageScope DX You open a case in Aperio ImageScope DX from your organization s medical device data system MDDS For information and instructions on using your MDDS contact your site administrator Aperi...

Page 10: ...3 Menu The menu provides access to commands tool visibility and options 4 Active eSlide The active eSlide is the eSlide open in the Viewer window If multiple eSlides are open in the Viewer window the...

Page 11: ...Draw a freehand annotation Ruler Measurement Create a measurement annotation of an area of interest Rectangle Draw a rectangle or hold down the Shift key to draw a square Ellipse Draw an oval or hold...

Page 12: ...erent Areas of the eSlide on page 15 9 Thumbnail Navigation box Represents the area currently shown in the Viewer window Drag the box around the Thumbnail to view the corresponding area of the eSlide...

Page 13: ...gs on your viewing station monitor is incorrect or if someone attempts to calibrate the monitor with a third party software Your eSlides will not display unless the monitor and viewing station are con...

Page 14: ...the eSlide you want to open A pushpin in the circle indicates the eSlide is open and pinned in the Viewer window To close the pinned eSlide click the circle again You can open up to four eSlides at on...

Page 15: ...Click the Slide Tray arrow in the upper right of the Slide Tray To view the Slide Tray click the arrow again View Different Areas of the eSlide The following describes different ways to move around t...

Page 16: ...le click anywhere on the eSlide to go to the maximum zoom level highest resolution Double click again to return to the previous zoom level Use the Zoom Slider Click the Zoom buttons Fit 4x 10x and so...

Page 17: ...4 File Close Case None Closes the image currently open in ImageScope Alt F4 File Exit Closes ImageScope DX Ctrl M Image Manual Synchronization Activates Manual Synchronization Ctrl P Tools Navigation...

Page 18: ...uare Shift Arrow key None None Moves image left right up and down one screen width at a time Note Pan and Zoom must be active to move the image with the Arrow keys Shift Ctrl None None Press while dra...

Page 19: ...You can open one case at a time in Aperio ImageScope DX When you open a case from your MDDS Aperio ImageScope DX automatically closes any previously opened case before it opens your next case It takes...

Page 20: ...To perform quality control on the eSlides follow these steps 1 Open the eSlides in Aperio ImageScope DX 2 Look at the entire image including the perimeter of the eSlide to confirm that the entire tiss...

Page 21: ...lide Viewing View an eSlide To view an eSlide using the method described previously follow these steps 1 With the eSlide open in Aperio ImageScope DX set the zoom to 4X by clicking 4x on the Zoom Slid...

Page 22: ...oom in closer The maximum zoom available depends on the magnification of the original scanned image The greatest number on the Zoom Slider is the scan magnification In this example the slide was scann...

Page 23: ...ars and select Annotation When you use an annotation tool it stays active until you click another tool to use After drawing an annotation you need to click the Pan tool to move to other areas of the e...

Page 24: ...at 20x may appear in millimeters at lower zoom level Draw a rectangle or square 1 Click Rectangle tool 2 Drag your mouse pointer to draw a rectangle To draw a square press and hold the Shift key as y...

Page 25: ...or of new annotations Annotations drawn previously remain in their original color To change the color of annotations 1 Click the Annotation Color Picker tool 2 When the Color window opens click the co...

Page 26: ...tting the fixed size for annotations see Annotation Options on page 36 Draw an Annotation with a Fixed Aspect Ratio You can draw an annotation that uses the same aspect ratio width and height proporti...

Page 27: ...like this Complete an annotation Edit an annotation Change eSlide Views This section describes how to perform some common navigation tasks Rotate the eSlide To rotate the eSlide from 0 to 360 drag th...

Page 28: ...tated with the eSlide If you draw new annotations on the rotated eSlide they are drawn relative to your current view In the example below the annotation on the right was drawn before the eSlide was ro...

Page 29: ...file location and name and then click Save to save the image You can open the saved TIFF file in Aperio ImageScope DX however note that the pan and zoom features are not available when viewing TIFF f...

Page 30: ...these steps to open and view multiple eSlides in the Aperio ImageScope DX Viewer window 1 Click the circle next to each eSlide you want to open Selected images appear in the Slide Tray with push pin...

Page 31: ...he Image toolbar and click the Manual Synchronization tool 4 Pan and zoom through the active eSlide Viewing a Z Stack eSlide The Aperio AT2 DX scanner can create multiple digital images of slide tissu...

Page 32: ...e eSlides The Focus slider shown below only appears on z stack eSlides You use this tool to view different z stack layers Viewing Different Z Stack Layers Drag the Focus slider up and down to view dif...

Page 33: ...rmation for each z stack layer Drawing and Viewing Annotations on a Z Stack eSlide Annotations are stored on the z stack layer on which you draw them Annotations are visible from all z stack layers To...

Page 34: ...To navigate through annotations on a z stack eSlide use the Next and Previous buttons As you navigate to each annotation the system adjusts the eSlide view to the corresponding z stack layer You must...

Page 35: ...ontains instructions for performing a visual quality check on your monitor View the Aperio ImageScope DX Options To view or set Aperio ImageScope DX options go to the Tools menu and select the option...

Page 36: ...re See Scrolling in the table on page 16 y y Pan Speed specifies how fast the image moves when you manually drag the image Select the Reverse panning direction pathologist mode check box to pan in rev...

Page 37: ...s made in this eSlide Minimum Resolution Specifies the minimum resolution of a view that is mapped This defines the lowest resolution at which a region of the image is viewed Minimum Magnification Spe...

Page 38: ...special test image Familiarizing yourself with the test image when you first use the system may help you more readily identify any changes in the image in the future Follow these steps to evaluate the...

Page 39: ...3 3 2 2 4 Check for the following items on the test image Test Graphic Area What to Check 1 Vertical gradient bars outlined in Blue Changes in intensity are smooth and uniform over the entire range of...

Page 40: ...rayscale square patch inside the black square is clearly visible at normal viewing distance 30 60 cm The small 5 grayscale square patch inside the white square is clearly visible at normal viewing dis...

Page 41: ...or general use instructions see the documentation and tutorials provided with your SpaceMouse Pro mouse 1 2 3 4 5 6 7 9 8 10 11 12 1 View at 2x 20x image or 4x 40x image 6 Place an observation pin ann...

Page 42: ...AT2 DX Viewing Station open Aperio ImageScope DX 2 Connect the SpaceMouse Pro device to your Aperio AT2 DX Viewing Station 3 On the SpaceMouse Pro device press the Menu key to open the properties pane...

Page 43: ...use Pro is now ready to use with Aperio ImageScope DX Rotate the eSlide with the SpaceMouse Pro Device To rotate the eSlide 1 Press the Rotate button on your SpaceMouse Pro device to turn on the rotat...

Page 44: ...24 Aperio AT2 DX Viewing Station viewing test image 38 area of interest view 22 axes show or hide 12 B best focused layer z stack 31 C camera tool See Snapshot tool case view eSlides 13 change eSlide...

Page 45: ...e DX close 18 example screen 9 indicator 9 12 overview 9 Image toolbar 9 10 See also Image tools Image tools eSlide information view 10 Snapshot 10 Synchronize 10 31 image types supported 7 Informatio...

Page 46: ...Scale Bar description 12 example 9 show or hide 12 scan magnification 16 22 Scanning Technician 20 scan slides See Aperio AT2 DX User s Guide screen expand 12 screen shot See Snapshot tool Scroll fea...

Page 47: ...ar 9 12 See also View tools View tools eSlide Label 12 28 Full screen 12 Rotation Dial 12 27 scalebar axes grid 12 Thumbnail 12 15 Zoom Slider 12 W window make full screen 12 Z zoom and pan multiple e...

Page 48: ...Aperio ImageScope DX User s Guide Revision A Leica Biosystems Imaging Inc 2019 48 Index...

Page 49: ...waste disposal Caution The lightning flash with arrowhead symbol within an equilateral triangle is intended to alert you to the presence of uninsulated dangerous voltage within the product s enclosur...

Page 50: ...www LeicaBiosystems com Aperio...

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