Leica Aperio ImageScope DX, User Manual

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Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019 20
Chapter 3: Basic eSlide Viewing
2. With the case opens in Aperio ImageScope DX, you can do the following:
Ì `
Perform Quality Control on the eSlides scanned for the case. See ”eSlide Quality Control” on page 20 .
Ì `
View the eSlide and change the zoom level (pan and zoom). See “Basic eSlide Viewing” on page 20.
Ì `
View one or more eSlides using advanced viewing techniques. See “Chapter 4: View and Annotate
eSlides” on page 23.
Ì `
Draw annotations on the eSlide to indicate areas of interest. See “Annotate eSlides” on page 23.
Close a Case in Aperio ImageScope DX
To close the eSlides for a case without closing Aperio ImageScope DX, go to the
File
menu, and select
Close Case
.
Note that Aperio ImageScope DX closes a previously opened case when you open a new case.
eSlide Quality Control
The scanning technician should perform quality control on the glass slides and eSlides during the scanning process. The
pathologist must also perform quality control on the eSlides.
To assess the quality of the slide preparation, follow the instructions for the specific staining protocol.
To perform quality control on the eSlides, follow these steps:
1.
Open the eSlides in Aperio ImageScope DX.
2.
Look at the entire image, including the perimeter of the eSlide, to confirm that the entire tissue sample was
scanned.
3.
Adjust the magnification using the Zoom tool. Click the thumbnail in the ImageScope window and pan the image.
4.
All eSlides should be in focus and free of visible image stripes.
5.
Rescan any eSlides that failed to scan or failed the image quality review.
If an eSlide has poor quality in any of the above areas, you may need to request a new slide or a re-scan of the slide.
For more details about preparing glass slides for scanning, see the Aperio AT2 DX User’s Guide .
Basic eSlide Viewing
Use the Aperio ImageScope DX navigation tools to move around the eSlide and to view areas of the eSlide at different zoom
levels. You do not need to focus the eSlide. Optimum focus is determined when the slide is scanned.
The steps below describe one method of reviewing eSlides:
1.
Adjust the zoom to
4x
and pan through the eSlide to look for areas of interest.
2.
When you find an area of interest, adjust the zoom to
20x
or
40x
to assess the area.
3.
Return to a lower zoom level and continue panning through the eSlide to look for more areas of interest.
The next section provides detailed steps for this viewing method.