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Leica BIOSYSTEMS HistoCore BIOCUT, Instructions For Use Manual

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Leica BIOSYSTEMS HistoCore BIOCUT Instructions For Use Manual Download Page 36

36

Version 1.7, Revision K 

 

Operation

5

5.2 

Inserting the two-in-one blade holder E

5.2.1  Setting up the blade holder base

Fig. 18 

 

1

2

3

4

5

1.  Release the clamping lever 

(

→

 "Fig. 18-

1

")

 by rotating it counterclockwise.

2.  Insert the blade holder base 

(

→

 "Fig. 18-

2

")

 using the notch 

(

→

 "Fig. 18-

3

")

 on the bottom into 

the T-piece 

(

→

 "Fig. 18-

5

")

 of the microtome base plate 

(

→

 "Fig. 18-

4

")

.

3.  The blade holder base 

(

→

 "Fig. 18-

2

")

 can be moved back and forth on the microtome base 

plate. This allows bringing the two-in-one blade holder E to optimal sectioning position in 
relation to the specimen.To secure the blade holder base, turn the clamping lever 

(

→

 "Fig. 

18-

1

")

 clockwise.

5.2.2  Inserting the two-in-one blade holder E

Fig. 19 

 

7

8

9

10

1.  Loosen the eccentric bolt 

(

→

 "Fig. 19-

9

")

 using a Hexagon key No. 4 

(

→

 "Fig. 19-

10

")

.

2.  Place the two-in-one blade holder E 

(

→

 "Fig. 19-

8

")

 with the underside groove onto the T-piece 

(

→

 "Fig. 19-

7

")

 of the knife holder base 

(

→

 "Fig. 18-

2

")

.

3.  To clamp, retighten the eccentric bolt 

(

→

 "Fig. 19-

9

")

.

Summary of Contents for HistoCore BIOCUT

Page 1: ...BIOCUT Rotary Microtome Instructions for Use English Order No 14 0517 80101 Revision K Always keep this manual with the instrument Read carefully before working with the instrument Version 1 7 Revision K 01 2022 ...

Page 2: ......

Page 3: ...al details of the present Instructions for Use are not to be considered warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and our customers Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice Only in this way is it possible to continuously improve the technology and...

Page 4: ... components 21 3 2 Instrument specifications 22 3 3 Technical Data 22 4 Setting up the instrument 25 4 1 Installation site requirements 25 4 2 Standard delivery packing list 25 4 3 Unpacking and installation 26 4 4 Assembling the handwheel 29 5 Operation 31 5 1 Operating elements and their functions 31 5 1 1 Section thickness setting 31 5 1 2 Coarse feed wheel 31 5 1 3 Specimen retraction 32 5 1 4...

Page 5: ...knife holder 54 6 3 1 Two in one blade holder E 54 6 3 2 Knife holder base without lateral displacement feature 56 6 3 3 Knife holder E with a water trough for low profile blades 56 6 3 4 Knife holder N 57 6 4 Section waste tray 59 6 5 Backlighting 59 6 6 Top tray 60 6 7 Universal microscope carrier 60 6 8 Magnifying lens LED illumination 63 6 9 Additional accessories 65 6 10 Ordering information ...

Page 6: ...6 Version 1 7 Revision K Table of Contents 9 Warranty and Service 86 9 1 Warranty 86 9 2 Service information 86 9 3 Decommissioning and disposal 86 10 Decontamination Confirmation 87 ...

Page 7: ...if not avoided could result in damage to the machine or something in its vicinity Tip Gives you tips to simplify your workflow Item numbers for numbering illustrations Fig 7 1 Numbers in red refer to item numbers in illustrations Manufacturer Order number Serial number Date of manufacture Observe the Instructions for Use Caution consult the instructions for use for cautionary information In vitro ...

Page 8: ...es the correct upright position of the package Indicates the temperature range permitted for transporting the package Minimum 29 C Maximum 50 C Indicates the temperature range permitted for storing the package Minimum 5 C Maximum 50 C Indicates the humidity range permitted for storing and transporting the package minimum 10 r H maximum 85 r H In the Shockwatch system the shock dot shows impacts or...

Page 9: ...It is intended for sectioning soft and hard human specimens as long as they are suitable for being cut manually The HistoCore BIOCUT is designed for in vitro diagnostic applications Warning Any use other than the intended use Serious injury of persons and or damage to specimen Follow the intended use described in the Instructions for Use Any other use of the instrument will be considered as improp...

Page 10: ...he operator s country Warning Protective devices or safety accessories provided by manufacturer have been removed or modified Serious injury of persons and or property damage including specimen damage Never remove or modify any protective devices of the instrument or any accessories Only service personnel qualified by Leica Biosystems may repair the instrument and access the instrument s internal ...

Page 11: ...the lifting points described in the Instructions for Use and Unpacking Instructions base plate at the front and under the instrument on the back Never lift the instrument by the handwheel grip coarse feed wheel object head or the knob for setting the section thickness Always remove the section waste tray before transporting the instrument Warning Careless moving of the instrument Serious injury of...

Page 12: ...t no oil is spilled If oil has been spilled clean it up immediately thoroughly and completely Warning Paraffin waste falls on the floor and is not cleaned up Serious injury of persons for example by slipping and stumbling into the knife blade Always clean up paraffin waste before it spreads becomes slippery and becomes a hazard Wear appropriate shoes Caution Accessories components loosening damage...

Page 13: ...sure that all protective devices and safety accessories are in place and fulfill their purpose properly Warning Personal protective equipment is not used Injury of persons When working with microtomes personal safety precautions must always be taken It is mandatory to wear work safety shoes safety gloves a mask and safety goggles Warning Knives or blades are handled and or disposed in an inappropr...

Page 14: ...and a minimum thickness of more than 0 246mm The recommended compatible blade is listed in Optional Accessories P 48 6 Optional Accessories Warning The blade is not installed completely parallel to the upper edge of the pressure plate due to excess paraffin uncleaned inserts etc When using the lateral movement function failure to install the blade parallel to the pressure plate may result in poor ...

Page 15: ...Operator can get cut and injured which can lead to serious consequences Always use cut resistant safety gloves when handling the specimen within the microtome Lock the handwheel and cover the knife edge with the safety guard prior to any manipulation of the specimen clamp and prior to changing the specimen Warning Specimen is oriented in retraction phase Specimen damage due to re orienting the spe...

Page 16: ...ient sections results Make sure that all prerequisites for appropriate sectioning are met Refer to the troubleshooting section of these Instructions for Use for further details If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who do have that knowledge for example application experts of Leica Biosystems Warning Wheel turn directi...

Page 17: ...ever is moved beyond this point it is possible that the handwheel is no longer braked Warning Fingers put between the specimen and the knife blade after the handwheel is released when doing fast manual trimming Operator can get cut and injured due to the turning of the handwheel when it is unlocked Do not put fingers between the specimen and the knife blade during trimming and sectioning Caution A...

Page 18: ...iquids do not enter the interior of the instrument during operation and maintenance Warning Wiping the knife in the wrong direction during cleaning Serious injury of persons Always wipe the knife from the back of the knife to the cutting edge Warning During cleaning knife blade holder remove the parts from the drying chamber 65 C Scalding hazard Wear heat insulating gloves when removing the parts ...

Page 19: ...re working with the instrument always assure that all protective devices and safety accessories are in place and fulfill their purpose properly 2 3 1 Locking the handwheel Fig 1 3 1 2 4 5 There are two ways of locking the handwheel Fig 1 1 Using the handwheel brake lever Fig 1 3 on the right side of the microtome base plate the handwheel can be braked in any position 1 To brake rotate the handwhee...

Page 20: ...older is equipped with a tightly mounted safety guard Fig 2 1 Fig 3 1 This makes it possible to cover completely the cutting edge in every knife or blade position Fig 2 1 2 2 Knife holder N The safety guard Fig 2 1 of the knife holder N can be easily positioned via two handles Fig 2 2 To cover the knife edge push both cover strips of the safety guard to the center Fig 3 1 2 3 Two in one blade hold...

Page 21: ... tray 8 Handwheel brake lever 2 Object head with orientation and UCC 9 Clamping lever of the knife blade holder base 3 Anti static waste tray 10 Coarse feed wheel 4 Window for displaying the section thickness 11 Trimming lever 5 Handwheel locking mechanism 12 Microtome base 6 Handwheel 13 Two in one blade holder E 7 Adjusting knob for setting the section thickness 14 Blade holder base ...

Page 22: ...ng with full hand wheel rotation Two independent hand wheel locking systems Operator adjustable force balancing system with spring force compensation offers two advantages 1 Flexibility to adapt the spring force to different weights of specimen clamps minimizing the risk of an object head dropping into the knife 2 No need for a heavy counter balance in the hand wheel Silent specimen retraction sys...

Page 23: ... off Trimming function with 2 settings 10 µm 30 µm Coarse feed wheel turn direction clockwise or counter clockwise User selectable Dimensions and weights Width including the handwheel and the coarse feed wheel 477 mm Depth including the section waste tray 620 mm Height without the top tray 295 mm Height with the top tray 303 mm Weight without accessories Approx 31 kg Volume of the section waste tr...

Page 24: ...cations 3 Operating environment Operating altitude Up to 2000 m above sea level Transport and storage environment Transport temperature range 29 C to 50 C Storage temperature range 5 C to 50 C Transport and storage relative humidity 10 to max 85 non condensing ...

Page 25: ... fixture for specimen clamp 14 0502 37717 1 Quick clamping system 14 0502 37718 1 Universal cassette clamp 14 0502 37999 1 Blade holder base 14 0502 55546 1 2 in 1 Blade holder E 14 0502 54497 HistoCore BIOCUT basic instrument includes the following delivery list Quantity Part description Order No HistoCore BIOCUT basic instrument 14 0517 56235 1 Handwheel assembly 14 0501 38181 1 Anti static wast...

Page 26: ...Blade holder base and Blade holder 14050255546 Blade holder base 14050254497 2 in 1 blade holder E Knife holder base and knife holders 14050237962 Knife holder base 14050237993 Knife holder N 14050238961 Knife holder E w trough low profile Additional optional accessories and knives blades can be found in Chapter 6 P 48 6 Optional Accessories Tip The accessories ordered are included in a separate b...

Page 27: ...strument and working surface Warning Instrument not positioned safely with all 4 feet on an appropriate laboratory table Serious injury of persons and or property damage Put the instrument only on a stable vibration free laboratory table with horizontal flat table top Ground needs to be vibration free as far as possible Always make sure that all 4 feet are positioned completely on the laboratory t...

Page 28: ...e instrument 4 Fig 6 1 2 Remove the packing strap and the adhesive tape Fig 6 1 Remove the carton lid Fig 6 2 Fig 7 3 4 4 4 Take out the accessory carton optional accessories Fig 7 3 and the cartons Fig 7 4 for the standard scope of delivery ...

Page 29: ...it out of the formed cushion Fig 8 9 The instrument illustration is provided as an example only Fig 9 10 11 12 Place the instrument on a stable laboratory table The two sliding elements Fig 9 10 located on the rear of the base plate make it easier to move the instrument on the table To move the instrument hold it by the front of the base plate Fig 9 11 lift it up gently and slide it on its slides ...

Page 30: ...nting the handwheel The instrument cannot be used and it may cause the delay of diagnosis Before assembling the handwheel check the feather key and make sure that it is in the handwheel shaft 2 Place the handwheel Fig 10 5 on the handwheel shaft Fig 10 1 as shown 3 Tighten the screw Fig 10 6 located in the center hole of the handwheel with a Hexagon key No 4 Fig 10 7 4 Remove the cover foil from t...

Page 31: ...he coarse feed wheel Fig 12 1 can be set as clockwise or counterclockwise to advance the object head The adjusting slot Fig 12 2 is located at the rear plate of the instrument Use the flat screw driver supplied with the delivery to turn the slot into horizontal position clockwise Fig 12 3 or vertical position counterclockwise Fig 12 4 The coarse motion serves for a fast horizontal forwards movemen...

Page 32: ...he retraction Off turn the slot into the horizontal position OFF For turning the retraction On turn the slot into the vertical position ON Before switching the specimen retraction On and Off run the object head to the upper end position by turning the handwheel 5 1 4 Mechanical trimming function Fig 14 1 2 The instrument is fitted with a mechanical trimming function The trimming lever has 3 notchi...

Page 33: ...ps available as optional accessories can be used Fig 15 1 2 3 4 4 The specimen orientation allows for simple position correction of the specimen surface when the specimen is clamped into place The directional specimen holder fixture may be exchanged for a non directional fixture optional accessory Display of the zero position For better display of the zero position the orientation has two red indi...

Page 34: ...rns 8 are possible in every direction The accuracy is approximately 0 5 For ease of estimation there is a white mark on the handle and a click stop that is noticeable during turning 4 To lock the current orientation turn the eccentric lever Fig 15 1 counterclockwise Tip When the large standard specimen clamp 50 x 55 mm or the super cassette clamp is used the specimen orientation of 8 in north sout...

Page 35: ...rform a fine adjustment of the force balance immediately Fig 17 1 The force balance is adjusted using the screw Fig 17 1 which can be accessed by removing the section waste tray on the bottom of the base plate of the microtome Use the Hexagon key No 5 with handle for the adjustment If the object head moves downwards turn the screw Fig 17 1 approx 1 2 turn clockwise each time If the object head mov...

Page 36: ...base Fig 18 2 can be moved back and forth on the microtome base plate This allows bringing the two in one blade holder E to optimal sectioning position in relation to the specimen To secure the blade holder base turn the clamping lever Fig 18 1 clockwise 5 2 2 Inserting the two in one blade holder E Fig 19 7 8 9 10 1 Loosen the eccentric bolt Fig 19 9 using a Hexagon key No 4 Fig 19 10 2 Place the...

Page 37: ... Loosen the screw Fig 20 3 using a Hexagon key No 4 Fig 20 6 until the two in one blade holder E Fig 20 2 can be moved 2 Move the two in one blade holder E until the index mark of the desired clearance angle coincides with the reference line on the blade holder base Enlarged detail Fig 20 shows a clearance angle setting of 5 Tips The recommended clearance angle setting for the two in one blade hol...

Page 38: ... as far as it will go Tip Since all specimen clamps available as accessories are equipped with the same kind of guide on the back they are inserted in the same way described here using the example of the cassette clamp 5 5 Clamping the specimen Warning The operator grabs into the knife or blade due to inappropriate working procedure Serious injury of persons when putting the specimen in place if k...

Page 39: ...ll the knife blade in the center of the knife blade holder Knives Blades must not exceed the edge of the knife blade holder Warning Too thick or thin blade used in the two in one blade holder Sample impaired Use a blade with a maximum thickness of less than 0 322mm and a minimum thickness of more than 0 246mm The recommended compatible blade is listed in Optional Accessories P 48 6 Optional Access...

Page 40: ...Fig 25 1 When using low profile blades the insert Fig 25 1 for low profile blades must first be placed in the two in one blade holder E and make sure that the bottom edge of the insert fits closely to the groove of the blade holder Two magnets are attached to the rear side of the insert Fig 24 1 They point away from the operator towards the rear pressure plate after the insert has been inserted wi...

Page 41: ...lade dispenser Fig 28 Fig 28 Warning Knives or blades are handled and or disposed in an inappropriate manner Serious injury of persons due to contact with extremely sharp knives and or blades Work especially thoroughly and carefully when handling knives and or blades Always wear dedicated clothes including cut resistant gloves when handling knives and or blades Dispose knives and blades always in ...

Page 42: ...heel is released when doing fast manual trimming Operator can get cut and injured due to the turning of the handwheel when it is unlocked Do not put fingers between the specimen and the knife blade during trimming and sectioning 1 Move the specimen to the rear end position by turning the coarse feed wheel 2 Push the knife blade holder on the knife blade holder base almost until it is just before t...

Page 43: ...mask and safety goggles Warning Working with the instrument and brittle specimen without appropriate protective clothes Serious injury of persons by splinters while cutting brittle specimen Always wear appropriate protective clothes including safety goggles and work especially carefully when cutting brittle specimen Warning Insufficient frame conditions for sectioning Specimen damage or poor secti...

Page 44: ...tion that contains acid ammonium hydroxide that contains alkali etc Accessories can malfunction Avoid corrosive strongly acidic alkaline reagent or solvent dripping onto the instrument surface or accessories If such reagent or solvent has dripped onto the instrument surface or accessories wipe off the residue and dry the accessories sufficiently as soon as possible If such reagent or solvent is us...

Page 45: ...ion of knife blade or specimen clamp prior to changing the specimen and during all work breaks Warning Uploading or unloading the specimen on the microtome without appropriate clothes and in an inappropriate manner Operator can get cut and injured which can lead to serious consequences Always use cut resistant safety gloves when handling the specimen within the microtome Lock the handwheel and cov...

Page 46: ...nd never try to catch a falling knife Warning Paraffin waste falls on the floor and is not cleaned up Serious injury of persons for example by slipping and stumbling into the knife blade Always clean up paraffin waste before it spreads becomes slippery and becomes a hazard Wear appropriate shoes 1 Move the specimen to the upper end position by turning the handwheel and engage the handwheel locking...

Page 47: ...47 HistoCore BIOCUT Setting up the instrument 5 ...

Page 48: ...r specimen clamps Before assembling the fixture for specimen clamps activate the handwheel locking mechanism 6 1 1 Rigid fixture for specimen clamps Tips Remove the rubber ring Fig 29 5 only after attaching the object head Fig 29 2 4 5 1 3 Screw the rigid fixture for specimen clamps Fig 29 4 onto the object head Fig 29 3 Remove the screw Fig 29 1 place the specimen holder fixture Fig 29 4 onto the...

Page 49: ...as shown and screw them in using a Hexagon key No 3 Insert the spring Fig 30 3 and pin Fig 30 4 with the flatter side into the thrust piece Fig 30 2 Completely screw in the thrust piece with a flat tip screwdriver Completely screw in the setscrews Fig 30 5 Fig 30 6 6 1 3 Fine directional fixture for specimen clamps Fig 31 1 3 4 2 Before the fine directional fixture for specimen clamps can be mount...

Page 50: ...ional fixture for specimen clamps with zero point indicators or the directional fixture for specimen clamps Screw the 4 screws Fig 32 2 into bore Fig 32 1 with a Hexagon key size 2 5 and tighten them 6 2 Specimen clamps and holders Tips All specimen clamps available as accessories can be integrated into the fine directional or directional or non directional specimen holder fixtures For how to inst...

Page 51: ... thickness compressed folded or stripped sections Do not continue sectioning if you recognize insufficient sections results Make sure that all prerequisites for appropriate sectioning are met Refer to the troubleshooting section of these Instructions for Use for further details If you do not have sufficient knowledge in regards of troubleshooting insufficient sectioning results consult persons who...

Page 52: ...lded on make sure that the broken edge left by removing the lid does not prevent the specimen from being securely clamped if necessary the specimen must be clamped horizontally Caution When using thin walled cassettes the cassettes become deformed or insecurely clamped into place or other problems because of the clamping system Damage to specimen Delayed diagnosis Be careful when using thin walled...

Page 53: ...ws Fig 35 2 with a Hexagon key No 3 Next insert the screw Fig 35 1 from the side and briefly tighten it with a Hexagon key No 4 Insert the super cassette clamp from the side on the left into the dovetail guide of the rigid fixture for specimen clamps and tighten screw Fig 35 1 Caution When using super cassette clamp the orientation is not set to position 0 when directional fixture for specimen cla...

Page 54: ... Fig 37 1 2 3 4 5 6 7 8 9 1 Blade ejector 6 Safety guard 2 Clamping top 7 Bolt 3 Clamping lever left 8 Clamping lever right 4 Segment arc 9 Pressure plate 5 Blade holder base The two in one blade holder optimized for use with Leica Biosystems disposable blades low profile blades L x H x W 80 0 05 mm x 8 0 0 1 mm x 0 254 0 008 mm high profile blades L x H x W 80 0 05 mm x 14 0 0 15 mm x 0 317 0 005...

Page 55: ...p rotate the lever Fig 38 1 clockwise Warning Too thick or thin blade used in the two in one blade holder Sample impaired Use a blade with a maximum thickness of less than 0 322mm and a minimum thickness of more than 0 246mm The recommended compatible blade is listed in Optional Accessories P 48 6 Optional Accessories Warning The blade is not installed completely parallel to the upper edge of the ...

Page 56: ...ackward as appropriate Secure the clamping mechanism by rotating the lever Fig 39 1 clockwise 6 3 3 Knife holder E with a water trough for low profile blades Fig 40 3 1 2 The knife holder E with a water trough Fig 40 is only for low profile blades The safety guard on knife holder E consists of a red foldaway handle Fig 40 1 To cover the cutting edge fold the safety guard handle Fig 40 1 clockwise ...

Page 57: ... using the glass slide 6 3 4 Knife holder N The knife holder N is appropriate for standard steel knives profile c and d up to 16 cm long The integrated height adjustment feature allows you to also use knives that have been resharpened numerous times Knife holder N Fig 42 For holding conventional knives up to 16 cm in length Fig 42 1 2 3 4 5 6 Mounting the knife support bar Push the safety guard Fi...

Page 58: ...knife Fig 43 1 can be inserted whereas the facet of profile d knife Fig 43 2 should face the operator Putting the knife wrongly will damage the specimen and the knife Knife height adjustment When adjusting the knife height the knife edge should be positioned as exactly as possible in the actual center of rotation of the knife holder The lay on edge Fig 42 6 of the rear clamping chucks serves as a ...

Page 59: ...ti static section waste tray offers ease of cleaning due to the anti static surface Push the section waste tray Fig 44 1 from the front to the microtome base plate Fig 44 2 until it is held in place by the two magnets Fig 44 3 on the front of the microtome base plate 6 5 Backlighting Tips The backlighting cannot be used with the blade holder base of the two in one blade holder E To use with backli...

Page 60: ... or the back lighting illumination is attached The instrument may malfunction leading to delayed diagnosis The orientation must be in position 0 and the cover for backlighting illumination must be detached NEVER use the super cassette clamp with backlighting illumination The force balance system has to be adjusted when using the super cassette clamp 6 6 Top tray Fig 46 1 The top tray is mounted on...

Page 61: ...48 4 Support plate large for BIOCUT MULTICUT and AUTOCUT Fig 48 5 Support plate small for NANOCUT R Fig 48 6 Hexagon key No 3 Fig 48 7 4 countersunk screws to install support plate Fig 48 8 Hexagon key size 8 Fig 49 Assembling the universal microscope carrier Attach baseplate Select the large Fig 48 5 or small Fig 48 4 support plate depending on the microtome to be used Attach the support plate to...

Page 62: ...olumn Fig 51 2 onto the baseplate from above and tighten with the Hexagon key No 8 Warning The universal microscope carrier tips Serious injury of persons After installing the vertical column immediately place the microtome onto the baseplate so that the front feet of the microtome are positioned in the shallow recesses Fig 51 19 Tips The lock washer must be positioned between the baseplate and ve...

Page 63: ...ember Fig 52 14 and tighten Tips For more information on connecting and using the microscope magnifying lens or cold light source please see the appropriate Instructions for Use 6 8 Magnifying lens LED illumination Tips The magnifying lens provides a 2x magnification and can be used with all HistoCore series rotary microtomes Fig 53 2 1 4 3 Open the screw Fig 53 3 on the horizontal arm of the micr...

Page 64: ...green lights Fig 54 8 on the high power spot controller Turn the control wheel Fig 54 9 to adjust the brightness of the spot lights Fig 54 5 1 6 7 2 3 4 8 9 Adjust the position of the magnifying lens to the specimen being processed The magnifying lens can be swiveled completely to the side if necessary Warning Magnifying lens is not covered when not working with it Serious injury of persons and or...

Page 65: ...ngth Height adjustment of the knife Separate clearance angle adjustment Movable safety guard Order No 14 0502 37993 Knife holder E with trough Fig 57 For low profile disposable blades dimensions L x H x W 80 0 05 mm x 8 0 0 1 mm x 0 254 0 008 mm silver with a water trough Quick clamp system with ability to shift the clamping top laterally 3 click stop positions make it easier to utilize the entire...

Page 66: ...54 0 008 mm and the blade dimensions for high profile blades L x H x W 80 0 05 mm x 14 0 0 15 mm x 0 317 0 005 mm For HistoCore BIOCUT Ability to shift the clamping top laterally 3 click stop positions make it easier to utilize the entire blade length The pressure plate is replaceable Color coded foldaway safety guard With blade ejector Order No 14 0502 54497 Disposable blades low profile Leica 81...

Page 67: ...38926 10 packages of 50 pcs 14 0358 38383 Knife 16 cm profile c steel Knife 16 cm profile d steel Fig 62 Knife 16 cm long profile c Note Knife case 14 0213 11140 included Order No 14 0216 07100 Knife 16 cm long profile d Note Knife case 14 0213 11140 included Order No 14 0216 07132 Knife case small Fig 63 Plastic variable for 1 or 2 knives of 10 to 16 cm length Order No 14 0213 11140 ...

Page 68: ...9 x 28 8 x 6 2 mm at maximum Order No 14 0502 37999 Standard specimen clamp Fig 65 50 x 55 mm with adapter silver Order No 14 0502 38005 Standard specimen clamp Fig 66 40 x 40 mm with adapter silver Order No 14 0502 37998 Specimen holder fixture directional Fig 67 Silver including installation hardware Note Quick clamping system 14 0502 37718 has to be ordered separately Order No 14 0502 38949 ...

Page 69: ...ixture fine directional Fig 69 Silver with 2 zero point indicators XY orientation 8 per direction click stops every 2 Note Quick clamping system 14 0502 37718 must be ordered separately Order No 14 0502 37717 Quick clamping system Fig 70 For specimen holder for use with the fine directional fixture for specimen clamps with zero point indicators 14 0502 37717 or the directional fixture for specimen...

Page 70: ...ximum Uniform microtome sections through electric cooling of the universal cassette clamp using the Leica RM CoolClamp Energy saving cooling through the heat dissipation system The patented force balance system of the HistoCore rotary microtomes enables the specimen clamps to be changed quickly and reliably without modifying the handwheel Antistatic material makes cleaning easier Can be retrofitte...

Page 71: ... Magnifier includes an adapter for holding the optional LED illumination Order No 14 0502 42790 Universal microscope carrier Fig 74 Universal assembly Order No 14 0502 40580 Backlighting Fig 75 Note For HistoCore BIOCUT and MULTICUT only together with the External power supply unit Backlighting 14 0500 31244 Backlighting cannot be used in connection with a Super Mega cassette clamp 14 0502 38967 O...

Page 72: ...r rotary microtome series HistoCore BIOCUT and MULTICUT The following adapters are included UK Europe USA Japan Australia Order No 14 0500 31244 Top tray Fig 77 For HistoCore rotary microtome series Order No 14 0517 56261 Section waste tray Fig 78 For HistoCore rotary microtome series Anti static section waste tray Order No 14 0517 56237 ...

Page 73: ...de holder base 14 0502 55546 2 in 1 blade holder E 14 0502 54497 Leica 819 disposable blades low profile 50 pack 80 0 05 mm x 8 0 0 1 mm x 0 254 0 008 mm L x H x W 14 0358 38925 Leica 819 disposable blades low profile 50 pack 14 0358 38382 Leica 818 disposable blades high profile 50 pack 80 0 05 mm x 14 0 0 15 mm x 0 317 0 005 mm L x H x W 14 0358 38926 Leica 818 disposable blades high profile 50 ...

Page 74: ...n only function with LED 1000 control unit Order No 14 6000 04825 14 6000 04826 LED 1000 control unit This module can only function with LED 1000 Hi power spots 2 arm Order No 14 6000 04826 14 6000 04825 Backlighting The backlighting can only be used with Knife holder base Order No 14 0502 37962 14 0502 38719 External power supply unit Backlighting 14 0500 31244 Top tray 14 0517 56261 Anti static ...

Page 75: ...ed properly Reclamp the blade knife holders or orientation Check that the cassette is securely clamped in the universal cassette clamp If the universal cassette clamp is soiled by paraffin clean the universal cassette clamp P 80 8 1 Cleaning the instrument When using cassettes whose lid is molded on check that the broken edge allows the cassette to be clamped securely if necessary remove burrs or ...

Page 76: ...ing problems may arise If while trying to clamp the cassette you realize that it is not securely clamped into place an alternative tensioning clamp must be used Laterally displace the knife blade holder or insert a new knife blade Insert a new pressure plate or use a new two in one blade holder E Readjust the pressure plate Systematically try several clearance angle adjustments until the optimum a...

Page 77: ...t clamping of specimen and or knife holder Retraction is switched off during sectioning of resin plastic embedded specimens Turn the handwheel at a slower speed Systematically decrease the clearance angle until the optimum adjustment is obtained Check all screw and clamp connections on the specimen holder system and the knife holder If necessary tighten the levers and screws Switch on retraction H...

Page 78: ...otate the clamping lever counterclockwise Fig 81 2 3 Pull out the bolt Fig 81 3 Fig 82 3 4 Remove the damaged pressure plate Fig 82 4 Tips Make sure that the insert of the clamping top is installed as shown Fig 82 5 in case it falls out If it is installed wrongly the pressure plate cannot be clamped 5 Install a new pressure plate 6 Insert the bolt Fig 82 3 7 Rotate clamping lever clockwise Fig 82 ...

Page 79: ... 2 5 mm Hexagon key at the setscrew Fig 83 1 so that the lever initially slips when the lever is activated Continue turning the setscrew Fig 83 1 a little bit approx 1 4 turn to the left or right then check that the lever no longer slips but also does not jam heavily 7 4 The setting of the clamping system of the clamp mount on the base plate Fig 84 1 2 3 4 5 6 To set the clamping distance from the...

Page 80: ... knife case Never place a knife anywhere with the cutting edge facing upwards and never try to catch a falling knife Warning Knife blade holder falling off the instrument Serious injury of persons and or property damage If the knife blade holder is not fixed for example during installation or cleaning pay special attention that the knife blade holder is not falling off Whenever possible fix the kn...

Page 81: ...al cassette clamp UCC and other accessories if necessary Before each cleaning carry out the following preparatory steps Raise the specimen clamp to the upper end position and activate the handwheel lock Remove the blade from the blade holder and insert it in the receptacle at the bottom of the dispenser or remove the knife from the knife holder and put it back in the knife case Remove knife holder...

Page 82: ...5 7 and take the pressure plate off 3 Remove the clamping top To do so rotate the clamping lever Fig 85 2 of the lateral displacement counterclockwise and pull it out sideways push the clamping top Fig 85 3 until it can be removed from the segment arc Fig 85 5 4 Loosen the eccentric bolt using Hexagon key No 4 and remove the segment arc from the blade holder base 5 Clean all parts of the two in on...

Page 83: ...te is parallel to and level with the rear edge of the clamping top Fig 85 3 Tips If the insert of the clamping top is installed wrongly the pressure plate cannot be clamped Universal cassette clamp Fig 86 1 2 3 Detach cassette clamp Fig 86 1 for a thorough cleaning removing all paraffin residues For cleaning do not use xylene Use xylene substitutes or paraffin removers The cassette clamp Fig 86 1 ...

Page 84: ...I Category II Number of sections per day 8 000 sections per day 8 000 sections per day Workload hours per day 5 hours per day 5 hours per day Sectioning speed Predominantly high sectioning speed Slow to medium sectioning speed Specimen material Working with soft and hard specimens Predominantly soft specimen material Maintenance Every 12 months Every 24 months 8 3 Lubricating the instrument Once a...

Page 85: ...lade holder E Fig 88 1 2 Clamping lever Fig 88 1 for the lateral displacement Clamping lever Fig 88 2 for the blade Universal cassette clamp Fig 89 1 2 Lubricate the spiral springs Fig 89 1 of the tension lever Fig 89 2 and move it back and forth several times ...

Page 86: ...nty terms of your Leica Biosystems sales organization or the organization from which you have purchased the contractual product shall apply exclusively 9 2 Service information If you are in need of technical customer support or spare parts please contact your Leica Biosystems representative or the Leica Biosystems dealer where you purchased the instrument Please provide the following information M...

Page 87: ... LeicaBiosystems com within the product menu This template has to be used for gathering all required data When returning a product a copy of the filled and signed confirmation has to be enclosed or passed on to the service technician The responsibility for products that are sent back without this confirmation or with an incomplete confirmation lies with the sender Returned goods that are considere...

Page 88: ...88 Version 1 7 Revision K Notes ...

Page 89: ......

Page 90: ... LeicaBiosystems com Version 1 7 Revision K 01 2022 Leica Biosystems Nussloch GmbH Heidelberger Strasse 17 19 D 69226 Nussloch Germany Tel 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems com ...

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