Labcyte Echo 520, User Manual, Page: 85 / 200

Manual Operation
4-3
Echo initialization
The Echo liquid handler performs a system initialization that includes the
following actions:

All three stages move to their home position:

Source stage moves front to rear on its Y axis.

Destination stage moves left to right on its X axis, front to rear on
its Y axis, up and down on its Z axis, and partially rotates on its
Theta axis.

Acoustic transducer stage moves left to right on its X axis, front to
rear on its Y axis, and up and down on its Z axis.
Note: The LCD screen reports each step of the homing sequence as it
occurs. Homing synchronizes the mechanical stages with the
controlling software.

Process door opens.

Destination stage extends, then retracts.

Process door closes.

Upper anti-static bar moves to its uppermost position, then both anti-
static bars turn off.
 Plate dryer nozzle moves to the down position.
 Coupler fluid nozzle moves to the down position.
 Power lamp turns on (green).
 Warning lamp turns off (yellow to clear).
 Fault lamp turns off (red to clear).
 Coupler fluid pump turns on.
 Power to the RF amplifier turns on.
Echo liquid handler warm-up
The Echo liquid handler requires a warm-up period after the power is
turned on. The RF amplifier and the acoustic transducer must reach a stable
operating temperature. If the instrument has been off for an extended
period of time, a 60-minute warm-up period is recommended to allow all
components to reach proper operating temperatures.
Notes: Echo liquid handler warm-up time depends on the starting
temperature of its internal components. Always allow sufficient time for
the Echo liquid handler to reach thermal stability. Fluid survey or
transfer precision and accuracy will be affected by using the Echo liquid
handler before it is warmed up.