Labcyte Echo 520, User Manual, Page: 32 / 200

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Echo Liquid Handler User Manual
1.3 What types of liquid transfer are supported?
The Echo liquid handler supports a variety of simple to complex microplate
liquid transfer protocols. The bundled software program allows a user to
set up the following types of protocol transfers:
Liquid transfer can be tailored to the user’s specific requirements.
Variables that are user selectable or definable include the following:
 Source microplate type (from the list of qualified plates)
 User-definable destination microplate or surface
 Transfer type (for example, interleaved or quadrant 384-well to
96-well transfers)
 Transfer volume
 Number of copies (how many times the transfer protocol is executed)
 Transfer map specifying exactly which wells, or regions of wells, will
be transferred
 Predefined DMSO/water concentration maps
These fluid transfer attributes and choices are defined in Chapter 5.
Table 1.1 Liquid transfer types
Source (Src)
Microplate
Destination
(Dest)
Microplate
Transfer map type
(full or partial)
384-well 96-well
destacking
1
quadrant
(4 regions from 1 src plate
to 4 dest plates)
1 Destacking: transferring liquid from a single source (src) plate to several
destination (dest) plates
destacking interleaved
(2x2, or every other
dest well)
384-well 384-well standard (1 src plate to 1 dest plate)
384-well 1536-well
stacking
2
quadrant
(4 src plates to 4 regions
on 1 dest plate)
2 Stacking: transferring liquid from several source plates to a single destination
plate
stacking interleaved
(2x2, or every other
dest well)
384-well 3456-well
stacking quadrant
(9 src plates to 9 regions
on 1 dest plate) stacking interleaved
(3x3, or every third dest
well)
1536-well 96-well
destacking quadrant
(16 regions from 1 src plate
to 16 dest plates) destacking interleaved
(4x4, or every fourth
dest well)
1536-well 384-well
destacking quadrant
(4 regions from 1 src plate
to 4 dest plates) destacking interleaved
(2x2, or every other
dest well)
1536-well 1536-well standard (1 src plate to 1 dest plate)